Differential activity of bone marrow hematopoietic stem cell subpopulations for EPC development and ischemic neovascularization

Kwon, Sang‐Mo; Lee, Yun-Kyung; Yokoyama, Ayumi; Jung, Sun Young; Masuda, Haruchika; Kawamoto, Atsuhiko; Lee, You Mie; Asahara, Takayuki

doi:10.1016/j.yjmcc.2011.04.007

Cited by 49 publications

(52 citation statements)

References 24 publications

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“…In EPC-CFA of cultured cells in all Combs, as previously indicated in mouse or human EPC-CFA [32][33][34][35][36][37], two types of EPC colonies were morphologically detected: PEPC-CFU, formed of small, round cells, and DEPC-CFU, formed of large, spindle-like cells (supplemental online Fig. 1A).…”

Section: Optimization Of Growth Factor/cytokine Comb For Qqcmentioning

confidence: 60%

“…Considering the necessity of creating a defined assay, a novel adhesive clonogenic assay for the quantitative and qualitative analysis of EPCs based on differentiation hierarchy has been developed recently [32][33][34][35][36][37]. The new EPC colony-forming assay (EPC-CFA) enabled the distinction and the definition of two different types of EPC-colony-forming units (EPC-CFUs), that is, primitive and definitive EPC-CFUs, composed of small and large cells, respectively.…”

Section: Protocols and Manufacturing For Cell-based Therapiesmentioning

confidence: 99%

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Development of Serum-Free Quality and Quantity Control Culture of Colony-Forming Endothelial Progenitor Cell for Vasculogenesis

Masuda

Iwasaki

Kawamoto

et al. 2012

Stem Cells Translational Medicine

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Quantitative and qualitative impairment of endothelial progenitor cells (EPCs) limits the efficacy of autologous cell therapy in patients with cardiovascular diseases. Here, we developed a serum-free quality and quantity control culture system for colony-forming EPCs to enhance their regenerative potential. A culture with serum-free medium containing stem cell factor, thrombopoietin, vascular endothelial growth factor, interleukin-6, and Flt-3 ligand was determined as optimal quality and quantity culture ( MEDICINE 2012;1:160 -171

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Section: Optimization Of Growth Factor/cytokine Comb For Qqcmentioning

confidence: 60%

Section: Protocols and Manufacturing For Cell-based Therapiesmentioning

confidence: 99%

Development of Serum-Free Quality and Quantity Control Culture of Colony-Forming Endothelial Progenitor Cell for Vasculogenesis

Masuda

Iwasaki

Kawamoto

et al. 2012

Stem Cells Translational Medicine

Self Cite

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“…Previous studies have suggested that stem cell samples used to treat myocardial infarction promote neo-vascularization, and that this may be responsible for the regenerative effects of these treatments [21,22,31,32]. To determine more conclusively whether HSC are responsible for this revascularization following bone marrow cell treatment, we analyzed the density of capillaries in the peri-infarct zone of infarcted hearts one month after treatment (Figure 5(a)).…”

Section: Analysis Of the Relative Contribution Of Hsc To Facilitationmentioning

confidence: 99%

Hematopoietic stem cells are a critical sub-population of whole bone marrow in the treatment of myocardial infarction

Davy¹,

Walker²,

Wong³

et al. 2013

SCD

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Recent studies suggest that whole bone marrow (WBM) derived stem cells may facilitate recovery following myocardial infarction. However, the sub-population of WBM responsible for recovery remains uncertain. By adjusting the abundance of CD34 + LinNeg cells in human bone marrow we examined the relative significance of hematopoietic stem cells (HSC) in the recovery of cardiac function in a murine model of induced myocardial infarction. Enrichment of HSC by ~100-fold in WBM transplanted into mice significantly increased recovery of heart function and reduced scar size compared to transplantation of WBM depleted in HSC by ~10-fold (P < 0.05, and P < 0.01 respectively). Peri-infarct capillary density was significantly increased in recipients of HSC-enriched samples (P < 0.01) or WBM samples (P < 0.01) compared to controls. These results strongly suggest a critical role for HSC in the effective treatment of myocardial infarction with human bone marrow, and imply that enrichment of HSC may markedly benefit the clinical application of WBM treatments.

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“…These results indicated that the adhesion of EPCs and their migration and in vitro tubule-like formation abilities were impaired potentially because of the influence of apo (a). Previous studies had shown that EPCs could repair injured vessels in ischemic tissues and promote the proliferation of new vessels (Kwon et al, 2011). To elucidate the mechanisms by which apo (a) impaired the angiogenesis of EPCs in vivo, each mouse received an intravenous injection with 3 · 10 5 (100 mL) normal EPCs labeled with molecular probe CM-Dil.…”

Section: Apo (A) Impaired Epcs Tubule-like Formation Homing and Angimentioning

confidence: 99%

Apolipoprotein (a) Impairs Endothelial Progenitor Cell-Mediated Angiogenesis

Ren

Zhang

et al. 2013

DNA and Cell Biology

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Improvement of blood flow and promotion of angiogenesis are important therapeutic measures for the treatment of ischemic peripheral vascular diseases. Since apolipoprotein (a) (apo (a)) is a glycoprotein with repetitive kringle domains exhibiting 75% to 98% structural homology with plasminogen (Plg), apo (a) may also have a negative effect on endothelial progenitor cell (EPC)-induced angiogenesis through Plg-like inhibitory effects on EPC proliferation, adhesion, migration, and angiogenesis. To evaluate the effect of apo (a) on EPCs-induced angiogenesis, EPCs were isolated from the bone marrow of apo (a) transgenic mice, wild-type litter mates, and normal mice. These cells were cultured without or with apo (a) before transplantation. Hindlimb ischemia models were surgically induced in mice, which then received an intravenous injection of 3 · 10 5 EPCs. At 3, 7, and 14 days post EPC transplantation, the adhesion, migration abilities, and capillary density in calf muscles were assessed. Results indicate that apo (a) significantly reduced the adhesion and migration abilities of EPCs. Furthermore, the tubule-like formation of EPCs on Matrigel gels was damaged. In vivo experiments showed the homing of EPCs to ischemic peripheral vascular, and the number of capillary vessels decreased significantly in apo(a) transgenic mice. This study demonstrated that apo (a) could attenuate the adhesion, migration, and homing abilities of EPCs and could impair the angiogenesis ability of EPCs.

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Differential activity of bone marrow hematopoietic stem cell subpopulations for EPC development and ischemic neovascularization

Cited by 49 publications

References 24 publications

Development of Serum-Free Quality and Quantity Control Culture of Colony-Forming Endothelial Progenitor Cell for Vasculogenesis

Development of Serum-Free Quality and Quantity Control Culture of Colony-Forming Endothelial Progenitor Cell for Vasculogenesis

Hematopoietic stem cells are a critical sub-population of whole bone marrow in the treatment of myocardial infarction

Apolipoprotein (a) Impairs Endothelial Progenitor Cell-Mediated Angiogenesis

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