Differential Activation of Cyclin and Cyclin-Dependent Kinase Genes by Adenovirus E1A12S cDNA Product

Cloning and Characterization of a GC-Box Binding Protein, G10BP-1, Responsible for Repression of the Rat Fibronectin Gene

“…18,1998 GC BOX BINDING BASIC-ZIPPER PROTEIN 4777 acid sequence (42 kDa) is larger than that of G10BP (ϳ30 kDa) estimated by SDS-PAGE under nonreducing conditions in the absence of DTT (43). The molecular mass of G10BP was presumably underestimated under the nonreducing conditions, since it has two Cys residues at codons 42 and 67 and may form a secondary structure.…”

The adenovirus E1A domains required for induction of DNA rereplication in G2/M arrested cells coincide with those required for apoptosis

“…The domains required for repression of the cyclin D1 promoter activity and induction of DNA rereplication in G 2 /M arrested cells were also analysed to inquire the correlation between induction of apoptosis and S phase events that might be altered, since EId10 cells expressing E1A and Ids undergo apoptosis after accumulation in S phase. E1A and Ids more or less inhibit cyclin D1 expression (Ishii et al, 1993;Lasorella et al, 1996), and cyclin D1 bind to PCNA, an essential accessory factor to DNA polymerase d and e (Cox, 1997). The results indicate that induction of apoptosis by E1A requires not only the N-terminal and CR1 regions but also the CR2 region.…”

“…Expression levels of cyclins and PCNA in synchronized EId10A and EId10B cells during progression of S phase were analysed, since expression of cyclin D1 is more or less inhibited by E1A (Ishii et al, 1993) and Id2 (Lasorella et al, 1996) and cyclin D1 associates with PCNA by forming quaternary complex with cyclin-dependent kinase (cdk) and p21 (Xiong et al, 1992). The levels were similarly analysed in synchronized g12 and YId24 cells as controls.…”

“…g12 was established by introducing rat 3Y1 cells (Kimura et al, 1975) with pMA12SG and express E1A 12S in response to dexamethasone (dex) (Ishii et al, 1993). The EId10 cell line was established by introducing g12 cells with pMTV-Id-1H and pMTV-Id-2H and express E1A 12S , Id-1H and Id-2H in response to dex.…”

Enhancer and silencer binding proteins involved in the rat cdc2 promoter activation at the G1/S boundary

“…cdc2 associates with cyclin A in the S phase and with cyclin B in the G 2 phase, and is activated at the end of G 2 by the phosphorylation and dephosphorylation of key amino acid residues, triggering entry into the M phase (Draetta & Beach 1988;Pines & Hunter 1989). Although cdc2 seems to play no apparent role in the G 1 -S phase transition, its expression is induced greatly at the G 1 /S boundary (Ishii et al 1993;Pagano et al 1993;Tsai et al 1993). An analysis of the human cdc2 promoter showed that its activity is negatively regulated during G 1 , presumably by pRB family members, since the E2F binding site is essential for this negative regulation (Dalton 1992).…”