Differential accumulation of transcripts for four tomato 1-aminocyclopropane-1-carboxylate synthase homologs under various conditions.

Yip, Wing Ho; Moore, Thomas; Yang, Shang Fa

doi:10.1073/pnas.89.6.2475

Cited by 144 publications

(76 citation statements)

References 18 publications

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“…To provide experimental evidence to support our hypothesis, we cloned nine cDNA fragments, including five members of the ACC synthase gene family, two of the ACC oxidase family, and two of the ethylene receptor family. Among the seven previously cloned genes for ACC synthase (Rottmann et al, 1991;Yip et al, 1992;Lincoln et al, 1993;Spanu et al, 1993;Olson et al, 1995;Oetiker et al, 1997), fragments of LE-ACS1B and LE-ACS5 could not be amplified by RT-PCR used in this study, even by the use of specific primers. Although the transcription of these two genes has been demonstrated in tomato roots and suspension cultures (Yip et al, 1992;Spanu et al, 1993;Oetiker et al, 1997), there is no evidence demonstrating their expression in the fruit.…”

Section: Discussionmentioning

confidence: 82%

“…LE-ACS5 was not amplified in the present study, suggesting a tissue-specific expression of each ACC synthase gene family. The transcript of LE-ACS3 has been detected in fruits (Yip et al, 1992) and suspension cultures (Oetiker et al, 1997). Among the members of the LE-ACS gene family studied, LE-ACS1A, LE-ACS3, and LE-ACS6 genes were expressed in the preclimacteric fruit, suggesting that system 1 ethylene in tomato fruit may be mediated via these three genes.…”

Section: Discussionmentioning

confidence: 99%

“…These genes have been isolated and structurally characterized and are differentially expressed in various tissues at different stages of development and in response to internal or external stimuli such as ripening, senescence, wounding, and auxin (Fluhr and Mattoo, 1996). In tomato fruit a large body of evidence demonstrates that massive ethylene production is responsible for increases in LE-ACS2, LE-ACS4, and LE-ACO1 transcripts (Van Der Straeten et al, 1990;Olson et al, 1991;Rottmann et al, 1991;Yip et al, 1992;Lincoln et al, 1993;Barry et al, 1996). Expression of these genes in preclimacteric tomato fruit is rapidly induced and/or enhanced by treatment with ethylene (Maunders et al, 1987;Rottmann et al, 1991;Lincoln et al, 1993).…”

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confidence: 99%

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Differential Expression and Internal Feedback Regulation of 1-Aminocyclopropane-1-Carboxylate Synthase, 1-Aminocyclopropane-1-Carboxylate Oxidase, and Ethylene Receptor Genes in Tomato Fruit during Development and Ripening

et al. 1998

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We investigated the feedback regulation of ethylene biosynthesis in tomato (Lycopersicon esculentum) fruit with respect to the transition from system 1 to system 2 ethylene production. The abundance of LE-ACS2, LE-ACS4, and NR mRNAs increased in the ripening fruit concomitant with a burst in ethylene production. These increases in mRNAs with ripening were prevented to a large extent by treatment with 1-methylcyclopropene (MCP), an ethylene action inhibitor. Transcripts for the LE-ACS6 gene, which accumulated in preclimacteric fruit but not in untreated ripening fruit, did accumulate in ripening fruit treated with MCP. Treatment of young fruit with propylene prevented the accumulation of transcripts for this gene. LE-ACS1A, LE-ACS3, and TAE1 genes were expressed constitutively in the fruit throughout development and ripening irrespective of whether the fruit was treated with MCP or propylene. The transcripts for LE-ACO1 and LE-ACO4 genes already existed in preclimacteric fruit and increased greatly when ripening commenced. These increases in LE-ACO mRNA with ripening were also prevented by treatment with MCP. The results suggest that in tomato fruit the preclimacteric system 1 ethylene is possibly mediated via constitutively expressed LE-ACS1A and LE-ACS3 and negatively feedback-regulated LE-ACS6 genes with preexisting LE-ACO1 and LE-ACO4 mRNAs. At the onset of the climacteric stage, it shifts to system 2 ethylene, with a large accumulation of LE-ACS2, LE-ACS4, LE-ACO1, and LE-ACO4 mRNAs as a result of a positive feedback regulation. This transition from system 1 to system 2 ethylene production might be related to the accumulated level of NR mRNA.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Differential Expression and Internal Feedback Regulation of 1-Aminocyclopropane-1-Carboxylate Synthase, 1-Aminocyclopropane-1-Carboxylate Oxidase, and Ethylene Receptor Genes in Tomato Fruit during Development and Ripening

et al. 1998

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“…ACS gene analysis of higher plants indicated that the ACS enzyme is encoded by a highly divergent multigene family, the members of which are subject to differential regulation by more than one inducer (Felix et al, 1991;Olson et al, 1991;Rottmann et al, 1991;Kim et al, 1992;Liang et al, 1992;Yip et al, 1992;Abel et al, 1995). However, in tomato at least two of the six members of the ACS gene family are responsible for the burst of ethylene production during fruit ripening (Lincoln et al, 1993), whereas expression of three of them can be strongly induced by an elicitor (Oetiker et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

An Allele of the Ripening-Specific 1-Aminocyclopropane-1-Carboxylic Acid Synthase Gene (ACS1) in Apple Fruit with a Long Storage Life1

et al. 1999

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An allele of the 1-aminocyclopropane-1-carboxylic acid (ACC) synthase gene (Md-ACS1), the transcript and translated product of which have been identified in ripening apples (Malus domestica), was isolated from a genomic library of the apple cultivar, Golden Delicious. The predicted coding region of this allele (ACS1-2) showed that seven nucleotide substitutions in the corresponding region of ACS1-1 resulted in just one amino acid transition. A 162-bp sequence characterized as a short interspersed repetitive element retrotransposon was inserted in the 5 -flanking region of ACS1-2 corresponding to position ؊781 in ACS1-1. The XhoI site located near the 3 end of the predicted coding region of ACS1-2 was absent from the reverse transcriptase-polymerase chain reaction product, revealing that exclusive transcription from ACS1-1 occurs during ripening of cv Golden Delicious fruit. DNA gel-blot and polymerase chain reaction analyses of genomic DNAs showed clearly that apple cultivars were either heterozygous for ACS1-1 and ACS1-2 or homozygous for each type. RNA gel-blot analysis of the ACS1-2 homozygous Fuji apple, which produces little ethylene and has a long storage life, demonstrated that the level of transcription from ACS1-2 during the ripening stage was very low.

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“…Liang et al 1992;Yip et al 1992;Ten Have and Woltering 1997;Arteca and Arteca 1999). Although to a lesser extent, such variability in tissue-and stimulus-specificity of ACO (e.g.…”

Section: Discussionmentioning

confidence: 99%

An auxin-responsive 1-aminocyclopropane-1-carboxylate synthase is responsible for differential ethylene production in gravistimulated Antirrhinum majus L. flower stems

Woltering

Балк

Nijenhuis-deVries

et al. 2004

Planta

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The regulation of gravistimulation-induced ethylene production and its role in gravitropic bending was studied in Antirrhinum majus L. cut flower stems. Gravistimulation increased ethylene production in both lower and upper halves of the stems with much higher levels observed in the lower half. Expression patterns of three different 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS) genes, an ACC oxidase (ACO) and an ethylene receptor (ETR/ERS homolog) gene were studied in the bending zone of gravistimulated stems and in excised stem sections following treatment with different chemicals. One of the ACS genes (Am-ACS3) was abundantly expressed in the bending zone cortex at the lower side of the stems within 2 h of gravistimulation. Am-ACS3 was not expressed in vertical stems or in other parts of (gravistimulated) stems, leaves or flowers. Am-ACS3 was strongly induced by indole-3-acetic acid (IAA) but not responsive to ethylene. The Am-ACS3 expression pattern strongly suggests that Am-ACS3 is responsible for the observed differential ethylene production in gravistimulated stems; its responsiveness to IAA suggests that Am-ACS3 expression reflects changes in auxin signalling. Am-ACS1 also showed increased expression in gravistimulated and IAA-treated stems although to a much lesser extent than Am-ACS3. In contrast to Am-ACS3, Am-ACS1 was also expressed in non-bending regions of vertical and gravistimulated stems and in leaves, and Am-ACS1 expression was not confined to the lower side cortex but evenly distributed over the diameter of the stem. Am-ACO and Am-ETR/ERS expression was increased in both the lower and upper halves of gravistimulated stems. Expression of both Am-ACO and Am-ETR/ERS was responsive to ethylene, suggesting regulation by IAAdependent differential ethylene production. Am-ACO expression and in vivo ACO activity, in addition, were induced by IAA, independent of the IAA-induced ethylene. IAA-induced growth of vertical stem sections and bending of gravistimulated flowering stems were little affected by ethylene or 1-methylcyclopropene treatments, indicating that the differential ethylene production plays no pivotal role in the kinetics of gravitropic bending.

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Differential accumulation of transcripts for four tomato 1-aminocyclopropane-1-carboxylate synthase homologs under various conditions.

Cited by 144 publications

References 18 publications

Differential Expression and Internal Feedback Regulation of 1-Aminocyclopropane-1-Carboxylate Synthase, 1-Aminocyclopropane-1-Carboxylate Oxidase, and Ethylene Receptor Genes in Tomato Fruit during Development and Ripening

Differential Expression and Internal Feedback Regulation of 1-Aminocyclopropane-1-Carboxylate Synthase, 1-Aminocyclopropane-1-Carboxylate Oxidase, and Ethylene Receptor Genes in Tomato Fruit during Development and Ripening

An Allele of the Ripening-Specific 1-Aminocyclopropane-1-Carboxylic Acid Synthase Gene (ACS1) in Apple Fruit with a Long Storage Life1

An auxin-responsive 1-aminocyclopropane-1-carboxylate synthase is responsible for differential ethylene production in gravistimulated Antirrhinum majus L. flower stems

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