Differential accumulation of sulfur-rich and sulfur-poor wheat flour proteins is affected by temperature and mineral nutrition during grain development

DuPont, Frances M.; Hurkman, William J.; Vensel, William H.; Chan, R.; López, Rocío; Tanaka, Charlene K.; Altenbach, Susan B.

doi:10.1016/j.jcs.2006.04.003

Cited by 94 publications

(98 citation statements)

References 35 publications

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“…This increase was already reported in response to environmental factors, as high temperature (Majoul et al, 2003;Dupont et al, 2006aDupont et al, , 2006b). …”

Section: Resultssupporting

confidence: 75%

Analysis of gluten proteins composition during grain filling in two durum wheat cultivars submitted to two water regimes

et al. 2014

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Durum wheat (Triticum turgidum L. subsp. durum) is one of the major crops in the Mediterranean basin, where water stress often occurs during grain filling which represents a critical stage for the synthesis and accumulation of storage proteins (gliadins and glutenins). The aim of the study is to evaluate, by two-dimensional gel electrophoresis (2DE SDS-PAGE), the storage proteins composition of two durum wheat cultivars (Ciccio and Svevo) cultivated in a growth chamber under two different water regimes (control and water deficit). At milk stage and physiological maturity, gluten proteins have been extracted and separated by 2DE SDS-PAGE. The analysis of the gels was performed by the software ImageMaster 2D Platinum (Amersham). The results showed differences in protein expression within the different gel regions between water regimes and cultivars; under water deficit the rate of protein accumulation was faster for all the protein regions, either at milk and physiological stage. Protein accumulation within high molecular weight (H) region resulted faster in Ciccio than in Svevo mainly in the control treatment. In the low molecular weight region between 48 and 35 kDa (L 48-35), the cultivar Ciccio showed a higher protein expression than Svevo. Furthermore under water deficit a marked increase in H region volume and a decrease in the L 48-35 region was observed only for Svevo; instead in Ciccio no change was observed showing this cultivar a greater stability on changing water regime. Further studies by the use of mass spectrometry are necessary to identify specific peptides relative to drought stress during grain filling as well as to investigate the relationships with technological quality.

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“…This increase was already reported in response to environmental factors, as high temperature (Majoul et al, 2003;Dupont et al, 2006aDupont et al, , 2006b). …”

Section: Resultssupporting

confidence: 75%

Analysis of gluten proteins composition during grain filling in two durum wheat cultivars submitted to two water regimes

et al. 2014

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“…Drought Fertilizer Majoul et al, 2003Dupont et al, 2006a,b Yang et al, 2011Yang et al 2011Grove et al, 2009Dupont et al, 2006a,b Altenbach et al, 2011 a-gliadins þ Wan et al (2008) used 55k Affymetrix oligonucleotide arrays to evaluate the effects of high temperature (28/20 C), drought, and high temperature combined with drought on gene expression (Table 4). In their experiments, treatments were applied from 15 DPA until 28 DPA.…”

Section: High Temperaturementioning

confidence: 99%

New insights into the effects of high temperature, drought and post-anthesis fertilizer on wheat grain development

Altenbach

2012

Journal of Cereal Science

102

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“…These complex events, carried out over a period of a few days, clearly indicate the need for 'foldase' catalysts and chaperones. Further, the storage protein processes represent internal ER stress, and external environmental factors also affect storage protein quantity and accumulation (Dupont et al 2006), further pointing to the possible involvement of foldases and chaperones. In support of this, PDI has been found sequestered in wheat PBs (Grynberg et al 1977;Roden et al 1982), and noted to be upregulated in seed development, co-localizing with the storage proteins in the PBs (Shimoni et al 1995b).…”

Section: Introductionmentioning

confidence: 99%

In silico identification and analysis of the protein disulphide isomerases in wheat and rice

Dorse

Bhave

2012

Biologia

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The protein disulphide isomerases (PDI) typically catalyse the formation and isomerization of disulphide bonds during the folding of nascent proteins. Some PDI isoforms may also have chaperone roles under house-keeping and/or stress conditions. Human PDIs and PDI-like (PDIL) proteins show a diverse array of catalytic and chaperone roles. However, understanding of the diversity and roles of plant PDILs is limited. This work aimed to identify the PDIL superfamilies in the two main cereals, rice and wheat, to identify candidates with potential roles in seed storage protein deposition and stress response processes. Searches of the rice genomic and wheat transcript assembly databases, with the Arabidopsis PDILs as queries, led to the identification of twenty two genomic loci in rice, encoding up to thirty two putative coding sequences, as well as twenty expressed sequence tags in wheat. The gene structures in rice ranged from 3 to 15 exons, the exon lengths ranging from 22 to 1,054 base pairs (bp) and the intron lengths from 74 to 1345 bp. The wheat TAs ranged from 584-2,444 bp and many sequences appeared to be orthologous to some of the rice loci. The putative proteins of both plants exhibited the characteristic thioredoxin active-site motif WCXXC, but significant diversity in the lengths of putative proteins and the composition or positions of functional domains therein. The PDILs thus fell into five major groups: PDIL1 (7 in rice; 4 in wheat); PDIL2 (9 rice; 4 wheat); PDIL5 (7 rice; 10 wheat); QSOXL (2 rice; 1 wheat); APRL (7 rice; 1 wheat). The analysis of the gene and putative protein sequences, the functional domains of the latter, and comparisons to literature have led to identification of a number of sequences with potential enzymatic and/or chaperone roles. Several of these appear to be candidates for key roles in seed storage protein folding, plant development and stress response processes in these important crops.

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Differential accumulation of sulfur-rich and sulfur-poor wheat flour proteins is affected by temperature and mineral nutrition during grain development

Cited by 94 publications

References 35 publications

Analysis of gluten proteins composition during grain filling in two durum wheat cultivars submitted to two water regimes

Analysis of gluten proteins composition during grain filling in two durum wheat cultivars submitted to two water regimes

New insights into the effects of high temperature, drought and post-anthesis fertilizer on wheat grain development

In silico identification and analysis of the protein disulphide isomerases in wheat and rice

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