Differential accumulation of silymarin induced by exposure of Silybum marianum L. callus cultures to several spectres of monochromatic lights

Younas, Muhammad; Drouet, Samantha; Nadeem, Muhammad; Giglioli-Guivarc’h, Nathalie; Hano, Christophe; Abbasi, Bilal Haider

doi:10.1016/j.jphotobiol.2018.05.018

Cited by 44 publications

(38 citation statements)

References 72 publications

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“…M. Younas et al, [39] reported that the maximum Peroxide (POD) and Superoxide (SOD) activity was recorded our experiment shows strongly relation with them. In our experiment total phenolics and avonoids was increased when compared with the controlled white color light conditions.…”

Section: Resultssupporting

confidence: 67%

Interactive Effects of Spectral Lights and Salt Stress on Production of Secondary Metabolites and Growth Behavior in Economically Important Brassica Rapa

Khan¹,

Iqbal

Ahmad³

et al. 2020

Preprint

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BackgroundEnvironmental factors like, temperature, humidity, light intensity, the supply of water, minerals, and CO2 influence the growth of a plant and biomass accumulation and production of secondary metabolites. Plant cell culture technologies have been effective tools for both studying and producing plant secondary metabolites under in vitro conditions and for plant improvement. The main objective of the current study was to investigate the biomass production and accumulation of secondary metabolites in Brassica rapa in response to synergism of abiotic factors including salt and colored lights.MethodsBrassica rapa sterilized seed were germinated and exposed to different colored lights (white as a control, yellow, blue, green and red) in combination with different concentrations of salt. The length of shoot, root and biomass (fresh and dry) were monitored during the developmental stages. Total phenolics and flavonoids content of the samples were also investigated. Data was analyzed using ANOVA-single factorial.ResultsResults revealed that plants exposed to the synergism of salt stress and spectral lights have shown negative effects on the seed germination. Shoot length (75 mm) increased, while root length (55 mm) was inhibited in plants exposed to synergism of spectral lights and different concentrations of salt when compared with controlled conditions (48 mm for shoot and 100 mm for root). However, the fresh weight of the plants was increased at lower concentration of salt than controlled. Lowest concentration of salt in synergism with green light showed the maximum response. Total phenolic content increased, while total flavonoid decreases with increasing salt concentration. Maximum total phenolic and flavonoids content were recorded in dark incubation with a high and medium concentration of salt respectively.ConclusionBased on these results, it was concluded that synergism of low salt concentration and colored lights are effective but high concentrations of salt in combination with colored lights inhibited the growth of Brassica rapa.

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Section: Resultssupporting

confidence: 67%

Interactive Effects of Spectral Lights and Salt Stress on Production of Secondary Metabolites and Growth Behavior in Economically Important Brassica Rapa

Khan¹,

Iqbal

Ahmad³

et al. 2020

Preprint

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“…Plants in stress conditions produce huge amount of reactive oxygen species which in turn harm vital organic molecules, thus limiting plant growth and development . However, plants are blessed with a defense mechanism which neutralizes the reactive oxygen species mediated harmful effects via low molecular weight antioxidants substrates along with antioxidative enzyme system .…”

Section: Resultsmentioning

confidence: 99%

“…However, several strategies have been developed to enhance biosynthesis of these BAIs in plant cell cultures to make the production process cost efficient. Elicitation of BAIs with several biotic and abiotic compounds/products is a famous strategy to date .…”

Section: Introductionmentioning

confidence: 99%

Salicylic acid‐enhanced biosynthesis of pharmacologically important lignans and neo lignans in cell suspension culture of Linum ussitatsimum L

Nadeem

Ahmad

Zahir

et al. 2018

Engineering in Life Sciences

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Linum usitatsimum L. (flax) is a perennial herb with magnitude of medicinal and commercial applications. In the present study, we investigated the effects of salicylic acid (SA) on biosynthesis of lignans (secoisolariciresinol diglucoside (SDG) and lariciresinol diglucoside (LDG)) and neolignans (dehydrodiconiferyl alcohol glucoside (DCG) and guaiacylglycerol‐β‐coniferyl alcohol ether glucoside (GGCG)) in cell cultures of flax. Moderate concentration of SA (50 μM) enhanced biomass accumulation (10.98 g/L dry weight (DW)), total phenolic content (37.81 mg/g DW), and antioxidant potential (87.23%) to two‐fold than their respective controls after 72 h of exposure. However, higher levels of total flavonoid content (5.32 mg/g DW) were noted after 48 h of exposure to 50 μM of SA. HPLC analyses revealed that 50 μM SA, significantly enhanced biosynthesis of SDG (7.95 mg/g DW), LDG (7.52 mg/g DW), DCG (54.90 mg/g DW), and GGCG (16.78 mg/g DW), which was almost 2.7, 1.8, 3.88, and 3.98 fold higher than their respective controls after 72 h of exposure time, respectively. These results indicated that moderate concentrations of SA had significant effects on biosynthesis and productivity of lignans and neolignans in cell culture of L. usitatissimum.

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“…As compared to control (51.7 μg/g), maximum taxifolin (136 μg/g) was recorded for 1.0 mg/L melatonin treated cultures grown under continuous light. Younas et al [67] also reported maximum taxifolin accumulation in S. marianum calli grown under continuous white light. Since silymarin contents are usually synthesized and derived from taxifolin in S. marianum, it is safe to assume that low level of taxifolin could be due to its conversion into silymarin contents [68,69].…”

Section: Effect Of Light and Melatonin On Silymarinmentioning

confidence: 86%

Interactive Effects of Light and Melatonin on Biosynthesis of Silymarin and Anti-inflammatory Potential in Callus Cultures of Silybum marianum L.

Shah¹,

Ullah²,

Drouet³

et al. 2019

Preprint

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Silybum marianum L. is a well-known medicinal herb, primarily used in liver protection. Light strongly affects several physiological processes along with secondary metabolites biosynthesis in plants. Herein, S. marianum was exploited for in vitro potential under different light regimes in the presence of melatonin. The optimum callogenic response occurred in combination of 1.0 mg/L α-naphthalene acetic acid and 0.5 mg/L 6-Benzylaminopurine under photoperiod. Continuous light associated with melatonin treatment increased total flavonoid content (TFC), total phenolic content (TPC) and antioxidant potential, followed by photoperiod and dark treatments. The increased level of melatonin has a synergistic effect on biomass accumulation under continuous light and photoperiod, while adverse effect was observed under dark condition. More detailed phytochemical analysis showed maximum total silymarin content (11.92 mg/g DW) when placed under continuous light + 1.0 mg/L melatonin. Individually, the level of silybins (A and B), silydianin, isolsilychristin and silychristin was found highest under continuous light. Anti-inflammatory activities were also studied and highest percent inhibition was recorded against 15-LOX for cultures cultivated under continuous light (42.33%). The current study helps to better understand the influence of melatonin and different light regimes on silymarin production as well as antioxidant and anti-inflammatory activities in S. marianum callus extracts.

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Differential accumulation of silymarin induced by exposure of Silybum marianum L. callus cultures to several spectres of monochromatic lights

Cited by 44 publications

References 72 publications

Interactive Effects of Spectral Lights and Salt Stress on Production of Secondary Metabolites and Growth Behavior in Economically Important Brassica Rapa

Interactive Effects of Spectral Lights and Salt Stress on Production of Secondary Metabolites and Growth Behavior in Economically Important Brassica Rapa

Salicylic acid‐enhanced biosynthesis of pharmacologically important lignans and neo lignans in cell suspension culture of Linum ussitatsimum L

Interactive Effects of Light and Melatonin on Biosynthesis of Silymarin and Anti-inflammatory Potential in Callus Cultures of Silybum marianum L.

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