Differential Accumulation of sHSPs Isoforms during Desiccation of the Resurrection Plant Haberlea rhodopensis Friv. under Optimal and High Temperature

Mihailova, Gergana; Tchorbadjieva, M.; Rakleova, Goritsa; Georgieva, Katya

doi:10.3390/life13010238

Cited by 2 publications

(1 citation statement)

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“…They act as ATP-independent molecular chaperones, preventing the irreversible aggregation of denatured proteins [69]. Fourteen sHSP isoforms are detected in H. rhodopensis leaves under severe dehydration in response to high-temperature stress [70]. Transcripts encoding HSPs are abundantly expressed under unstressed conditions in vegetative tissues of H. rhodopensis, suggesting the readiness of the plant to combat stress [71].…”

Section: Discussionmentioning

confidence: 99%

Antioxidative Defense, Suppressed Nitric Oxide Accumulation, and Synthesis of Protective Proteins in Roots and Leaves Contribute to the Desiccation Tolerance of the Resurrection Plant Haberlea rhodopensis

Georgieva

Mihailova

Gigova

et al. 2023

Plants

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The desiccation tolerance of plants relies on defense mechanisms that enable the protection of macromolecules, biological structures, and metabolism. Although the defense of leaf tissues exposed to solar irradiation is challenging, mechanisms that protect the viability of the roots, yet largely unexplored, are equally important for survival. Although the photosynthetic apparatus in leaves contributes to the generation of oxidative stress under drought stress, we hypothesized that oxidative stress and thus antioxidative defense is also predominant in the roots. Thus, we aimed for a comparative analysis of the protective mechanisms in leaves and roots during the desiccation of Haberlea rhodopensis. Consequently, a high content of non-enzymatic antioxidants and high activity of antioxidant enzymes together with the activation of specific isoenzymes were found in both leaves and roots during the final stages of desiccation of H. rhodopensis. Among others, catalase and glutathione reductase activity showed a similar tendency of changes in roots and leaves, whereas, unlike that in the leaves, superoxide dismutase activity was enhanced under severe but not under medium desiccation in roots. Nitric oxide accumulation in the root tips was found to be sensitive to water restriction but suppressed under severe desiccation. In addition to the antioxidative defense, desiccation induced an enhanced abundance of dehydrins, ELIPs, and sHSP 17.7 in leaves, but this was significantly better in roots. In contrast to leaf cells, starch remained in the cells of the central cylinder of desiccated roots. Taken together, protective compounds and antioxidative defense mechanisms are equally important in protecting the roots to survive desiccation. Since drought-induced damage to the root system fundamentally affects the survival of plants, a better understanding of root desiccation tolerance mechanisms is essential to compensate for the challenges of prolonged dry periods.

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Section: Discussionmentioning

confidence: 99%