Differential accumulation of S-adenosylmethionine synthetase transcripts in response to salt stress

Espartero, Joaquín; Pintor‐Toro, José Antonio; Pardo, José M.

doi:10.1007/bf00023239

Cited by 177 publications

(120 citation statements)

References 54 publications

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“…The amount of this enzyme significantly decreased in the sample treated with moderate osmotic stress. A similar effect was also reported in other proteomic (Yan et al 2005;Toorchi et al 2009;Mohammadi et al 2012b) and transcriptomic (Espartero et al 1994) studies. This synthetase is probably involved in the control of gene expression under osmotic stress conditions due to its ability to methylate nucleic acids and, therefore, may constitute an interesting target for genetic engineering of plant resistance to this stress (Mohammadi et al 2012b).…”

Section: Discussionsupporting

confidence: 89%

“…Decrease in HSP70 expression in the triticale roots suggests, therefore, some disturbances in protein metabolism, perhaps caused by weak plant adaptation to stressful conditions. S-adenosylmethionine synthetase is an enzyme essential for DNA, RNA and protein methylation, biosynthesis of cell wall components and, indirectly, for the synthesis of polyamines and ethylene (Espartero et al 1994;Grillo and Colombatto 2008). It was revealed that the increased synthesis of polyamines (whose production depends on this synthase) increases tolerance of some plants to the osmotic stress (Wi et al 2006).…”

Section: Discussionmentioning

confidence: 99%

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Changes in the root proteome of Triticosecale grains germinating under osmotic stress

2013

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Osmotic stress causes many adverse symptoms in plants, which include, for example, growth limitation and decrease or even absence of yield. Proteomic analyses of plant responses to stressors could lead to the introduction of crops with high resistance to osmotic stress. Such plants would be characterized by high yield even under unfavorable environmental conditions. In this article we describe changes in the protein profiles occurring in response to mild and moderate osmotic stress in triticale roots. Analysis of the protein profiles of these roots showed an increased abundance of 14 and a decreased abundance of 11 proteins under mild osmotic stress conditions while a moderate osmotic stress caused an increased abundance of 18 and a decreased abundance of 33 proteins. Twenty-five proteins, whose quantity altered under stress were identified using MALDI-TOF mass spectrometry. The identified proteins were classified into the categories of proteins associated with: defense mechanisms, metabolism, transcription, cell structure, protein synthesis, transport and signal transduction. The functions of identified proteins were discussed in relation to osmotic stress. Some of the identified proteins may be responsible for the adaptation of plants to adverse conditions.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

2013

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“…S-adenosyl-L-methionine serves as the major methyl-group donor, for example, in the synthesis of secondary products (Yang and Hoffman, 1984;Heby and Persson, 1990). Transcript 1465, which is identical to a second rice SAMS (Z29867), was not downregulated, indicating that SAMS isoforms are differentially regulated by salt stress, as has been seen for other species (Espartero et al, 1994;Schröder et al, 1997).…”

Section: The Time-specific Response Of Transcripts In Pokkalimentioning

confidence: 82%

Gene Expression Profiles during the Initial Phase of Salt Stress in Rice

et al. 2001

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Transcript regulation in response to high salinity was investigated for salt-tolerant rice (var Pokkali) with microarrays including 1728 cDNAs from libraries of salt-stressed roots. NaCl at 150 mM reduced photosynthesis to one tenth of the prestress value within minutes. Hybridizations of RNA to microarray slides probed for changes in transcripts from 15 min to 1 week after salt shock. Beginning 15 min after the shock, Pokkali showed upregulation of transcripts. Approximately 10% of the transcripts in Pokkali were significantly upregulated or downregulated within 1 hr of salt stress. The initial differences between control and stressed plants continued for hours but became less pronounced as the plants adapted over time. The interpretation of an adaptive process was supported by the similar analysis of salinity-sensitive rice (var IR29), in which the immediate response exhibited by Pokkali was delayed and later resulted in downregulation of transcription and death. The upregulated functions observed with Pokkali at different time points during stress adaptation changed over time. Increased protein synthesis and protein turnover were observed at early time points, followed by the induction of known stress-responsive transcripts within hours, and the induction of transcripts for defenserelated functions later. After 1 week, the nature of upregulated transcripts (e.g., aquaporins) indicated recovery.

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“…Elevated mRNA levels for SAM synthetase and ethylene biosynthetic enzymes were apparent within 0.5 to 2 h after the onset of ozone exposure ( Figure 5). When RNA isolated from ozone-exposed tomato leaves was hybridized to genespecific SAM synthetase probes (Espartero et al, 1994), elevated transcript levels were detected for the main leaf isoform, SAM3, at 2 h ( Figure 5a). Transcript levels returned to the levels of control plants after 5 h. Induction of the SAM3 gene after 2 h of ozone exposure (200 nl 1-1) was also observed in the tomato cultivar Pearson when exposed under identical conditions ( Figure 5c): Transcript levels of SAM1 were not affected by ozone, and those of SAM2 were slightly reduced in treated as well as control plants under the experimental conditions used (Figure 5a).…”

Section: Resultsmentioning

confidence: 99%

“…Ozone treatment induced expression of specific SAM and ethylene biosynthetic genes in the experimental plants. Increases at the mRNA level were specifically detected for only one out of three SAM synthetase genes, SAM3 (Espartero et aL, 1994), and one out of four ACC synthase genes, LE-ACS2 (Rottmann et aL, 1991;Yip et aL, 1992).…”

Section: Discussionmentioning

confidence: 97%

Ozone induction of ethylene emission in tomato plants: regulation by differential accumulation of transcripts for the biosynthetic enzymes

et al. 1997

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SummaryStress ethylene emission is positively correlated with ozone sensitivity in various plant species, indicating that ethylene may be involved in the control of ozone damage. This study shows that ozone exposure of tomato plants for 5 h at 85 nl 1-1 and above leads to leaf injury within 24 h. 1-aminocyclopropane-l-carboxylic acid (ACC) content and ACC synthase activity were accordingly elevated within 1-2 h. Pre-treatment of leaves with inhibitors of ACC synthase and ACC oxidase significantly inhibited the evolution of ethylene and reduced ozone-induced visible damage. Transcript levels for only one out of three SadenosyI-L-methionine (SAM) synthetase genes (SAM3), and one out of four ACC synthase genes (LE-ACS2) were induced by ozone (maximum at 2 h). Treatment with protein kinase (K-252a) and phosphatase inhibitors (calyculin A) revealed that ACC synthase activity was additionally regulated by protein phosphorylation/dephosphorylation. Transcripts of ACC oxidase (pTOM13 cDNA probe) displayed the fastest response of the parameters tested (maximum at 30 min), suggesting a regulatory role for ACC oxidase in ethylene formation of ozone-exposed plants. The results demonstrate a highly selective ozone response by ethylene biosynthetic genes which resembles that of plant-pathogen interactions.

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Differential accumulation of S-adenosylmethionine synthetase transcripts in response to salt stress

Cited by 177 publications

References 54 publications

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

Gene Expression Profiles during the Initial Phase of Salt Stress in Rice

Ozone induction of ethylene emission in tomato plants: regulation by differential accumulation of transcripts for the biosynthetic enzymes

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