Differential accumulation of plastid transcripts encoding photosystem II components in the mesophyll and bundle-sheath cells of monocotyledonous NADP-malic enzyme-type C4 plants

Westhoff, Peter; Offermann-Steinhard, Karin; Hofer, Michaël; Eskins, Kenneth; Oswald, Angela; Streubel, Monika

doi:10.1007/bf00195340

Cited by 55 publications

(50 citation statements)

References 66 publications

(77 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the isolation of total RNA, the second and fourth visible leaves from the top of 10 plants each were harvested in the middle of the light period and immediately frozen in liquid nitrogen. Total RNA was isolated as described earlier (Westhoff et al, 1991). Poly(A + ) RNA was enriched by two consecutive rounds of oligo(dT) purification with the Oligotex mRNA Midi kit (Qiagen).…”

Section: Comparison To C 4 Photosynthesis In the Genus Cleome: Commonmentioning

confidence: 99%

Evolution of C4 Photosynthesis in the GenusFlaveria: How Many and Which Genes Does It Take to Make C4?

et al. 2011

Self Cite

View full text Add to dashboard Cite

Selective pressure exerted by a massive decline in atmospheric CO 2 levels 55 to 40 million years ago promoted the evolution of a novel, highly efficient mode of photosynthetic carbon assimilation known as C 4 photosynthesis. C 4 species have concurrently evolved multiple times in a broad range of plant families, and this multiple and parallel evolution of the complex C 4 trait indicates a common underlying evolutionary mechanism that might be elucidated by comparative analyses of related C 3 and C 4 species. Here, we use mRNA-Seq analysis of five species within the genus Flaveria, ranging from C 3 to C 3 -C 4 intermediate to C 4 species, to quantify the differences in the transcriptomes of closely related plant species with varying degrees of C 4 -associated characteristics. Single gene analysis defines the C 4 cycle enzymes and transporters more precisely and provides new candidates for yet unknown functions as well as identifies C 4 associated pathways. Molecular evidence for a photorespiratory CO 2 pump prior to the establishment of the C 4 cycle-based CO 2 pump is provided. Cluster analysis defines the upper limit of C 4 -related gene expression changes in mature leaves of Flaveria as 3582 alterations.

show abstract

Section: Comparison To C 4 Photosynthesis In the Genus Cleome: Commonmentioning

confidence: 99%

Evolution of C4 Photosynthesis in the GenusFlaveria: How Many and Which Genes Does It Take to Make C4?

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…All plant species were grown next to each other in the greenhouse, RNA was isolated as described (Westhoff et al, 1991), and construction of sequence libraries and sequencing followed Illumina protocols. Between 30 and 58 million reads could be obtained per species and experiment (J. Mallmann, U. Gowik, and P. Westhoff, unpublished data).…”

Section: Mapping and Quantification Of Readsmentioning

confidence: 99%

Evolution of C4 Photosynthesis in the Genus Flaveria: Establishment of a Photorespiratory CO2 Pump

et al. 2013

View full text Add to dashboard Cite

C 4 photosynthesis is nature's most efficient answer to the dual activity of ribulose-1,5-bisphosphate carboxylase/oxygenase and the resulting loss of CO 2 by photorespiration. Gly decarboxylase (GDC) is the key component of photorespiratory CO 2 release in plants and is active in all photosynthetic tissues of C 3 plants, but only in the bundle sheath cells of C 4 plants. The restriction of GDC to the bundle sheath is assumed to be an essential and early step in the evolution of C 4 photosynthesis, leading to a photorespiratory CO 2 concentrating mechanism. In this study, we analyzed how the P-protein of GDC (GLDP) became restricted to the bundle sheath during the transition from C 3 to C 4 photosynthesis in the genus Flaveria. We found that C 3 Flaveria species already contain a bundle sheath-expressed GLDP gene in addition to a ubiquitously expressed second gene, which became a pseudogene in C 4 Flaveria species. Analyses of C 3 -C 4 intermediate Flaveria species revealed that the photorespiratory CO 2 pump was not established in one single step, but gradually. The knowledge gained by this study sheds light on the early steps in C 4 evolution.

show abstract

“…RNA isolation and RNA gel blot analysis of total leaf RNA was performed as described previously (Westhoff et al, 1991;Meurer et al, 1996a). Hybridization probes were made by cloning either PCR fragments or restriction fragments, as described by Felder et al (2001).…”

Section: Rna Gel Blot Hybridization Analysismentioning

confidence: 99%

HCF152, an Arabidopsis RNA Binding Pentatricopeptide Repeat Protein Involved in the Processing of Chloroplast psbB-psbT-psbH-petB-petD RNAs

et al. 2003

View full text Add to dashboard Cite

The psbB-psbT-psbH-petB-petD operon of higher plant chloroplasts is a heterogeneously composed transcriptional unit that undergoes complex RNA processing events until the mature oligocistronic RNAs are formed. To identify the nucleusencoded factors required for the processing and expression of psbB-psbT-psbH-petB-petD transcripts, we performed mutational analysis using Arabidopsis. The allelic nuclear mutants hcf152-1 and hcf152-2 were identified that are affected specifically in the accumulation of the plastidial cytochrome b 6 f complex. In both mutants, reduced amounts of spliced petB RNAs (encoding the cytochrome b 6 subunit) were detected, thus explaining the observed protein deficiencies. Additionally, mutant hcf152-1 is affected in the accumulation of transcripts cleaved between the genes psbH and petB . As a result of a close T-DNA insertion, the HCF152 gene was cloned and its identity confirmed by complementation of homozygous mutant plants. HCF152 encodes a pentatricopeptide repeat (PPR) protein with 12 putative PPR motifs that is located inside the chloroplast. The protein shows a significant structural, but not primary, sequence similarity to the maize protein CRP1, which is involved in the processing and translation of the chloroplast petD and petA RNAs. In addition, we found that HCF152 is an RNA binding protein that binds certain areas of the petB transcript. The protein possibly exists in the chloroplast as a homodimer and is not associated with other proteins to form a high molecular mass complex.

show abstract

Differential accumulation of plastid transcripts encoding photosystem II components in the mesophyll and bundle-sheath cells of monocotyledonous NADP-malic enzyme-type C4 plants

Cited by 55 publications

References 66 publications

Evolution of C4 Photosynthesis in the GenusFlaveria: How Many and Which Genes Does It Take to Make C4?

Evolution of C4 Photosynthesis in the GenusFlaveria: How Many and Which Genes Does It Take to Make C4?

Evolution of C4 Photosynthesis in the Genus Flaveria: Establishment of a Photorespiratory CO2 Pump

HCF152, an Arabidopsis RNA Binding Pentatricopeptide Repeat Protein Involved in the Processing of Chloroplast psbB-psbT-psbH-petB-petD RNAs

Contact Info

Product

Resources

About