Differential accumulation of Arabidopsis thaliana Sbe2.1 and Sbe2.2 transcripts in response to light

Khoshnoodi, Jamshid; Larsson, Clas-Tomas; Larsson, Håkan; Rask, Lars

doi:10.1016/s0168-9452(98)00093-4

Cited by 11 publications

(6 citation statements)

References 33 publications

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“…However, when similar detached leaves were submerged in a 100 mM sucrose solution for 24 h in the dark, ApL3 expression was reduced in isi1 and isi2, was similar to the control line in isi3, and was almost undetectable in isi4 (visible only after prolonged exposure). This behaviour was not restricted to the ApL3 gene, but was also observed for another sucrose-inducible starch biosynthetic gene, Sbe2.2, encoding a starch-branching enzyme (Khoshnoodi et al, 1998). A possible explanation for these results is that the petiole-fed leaves have high water evaporation rates and could be subject to dehydration stress (although they were not severely wilted), which would lead to higher ABA levels.…”

Section: Sugar Induction Of Starch Biosynthetic Genes Is Enhanced By Abamentioning

confidence: 54%

Impaired sucrose‐induction mutants reveal the modulation of sugar‐induced starch biosynthetic gene expression by abscisic acid signalling

Rook¹,

Corke²,

Card³

et al. 2001

The Plant Journal

375

351

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SummaryPlants both produce and utilize carbohydrates and have developed mechanisms to regulate their sugar status and co-ordinate carbohydrate partitioning. High sugar levels result in a feedback inhibition of photosynthesis and an induction of storage processes. We used a genetic approach to isolate components of the signalling pathway regulating the induction of starch biosynthesis. The regulatory sequences of the sugar inducible ADP-glucose pyrophosphorylase subunit ApL3 were fused to a negative selection marker. Of the four impaired sucrose induction (isi) mutants described here, two (isi1 and isi2) were speci®c to this screen. The other two mutants (isi3 and isi4) showed additional phenotypes associated with sugarsensing screens that select for seedling establishment on high-sugar media. The isi3 and isi4 mutants were found to be involved in the abscisic acid signalling pathway. isi3 is allelic to abscisic acid insensitive4 (abi4), a gene encoding an Apetala2-type transcription factor; isi4 was found to be allelic to glucose insensitive1 (gin1) previously reported to reveal cross-talk between ethylene and glucose signalling. Here we present an alternative interpretation of gin1 as an allele of the ABA-de®cient mutant aba2. Expression analysis showed that ABA is unable to induce ApL3 gene expression by itself, but greatly enhances ApL3 induction by sugar. Our data suggest a major role for ABA in relation to sugar-signalling pathways, in that it enhances the ability of tissues to respond to subsequent sugar signals.

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Section: Sugar Induction Of Starch Biosynthetic Genes Is Enhanced By Abamentioning

confidence: 54%

Impaired sucrose‐induction mutants reveal the modulation of sugar‐induced starch biosynthetic gene expression by abscisic acid signalling

Rook¹,

Corke²,

Card³

et al. 2001

The Plant Journal

375

351

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“…Similar elements are also present in the promoter region of the SBE2.1 gene of Arabidopsis encoding a type II starchbranching enzyme and that has been recently shown to be light regulated (Khoshnoodi et al, 1998). The presence of these elements in the GBSSI promoter region suggests that fluctuations on the GBSSI mRNA abundance observed in leaves could be mediated by a circadian regulation of the gene transcription.…”

Section: Discussionmentioning

confidence: 83%

Expression of the Granule-Bound Starch Synthase I (Waxy) Gene from Snapdragon Is Developmentally and Circadian Clock Regulated1

et al. 1999

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The granule-bound starch synthase I (GBSSI or waxy) enzyme catalyzes one of the enzymatic steps of starch synthesis. This enzyme is responsible for the synthesis of amylose and is also involved in building the final structure of amylopectin. Little is known about expression of GBSSI genes in tissues other than storage organs, such as seeds, endosperm, and tuber. We have isolated a gene encoding the GBSSI from snapdragon (Antirrhinum majus). This gene is present as a single copy in the snapdragon genome. There is a precise spatial and developmental regulation of its expression in flowers. GBSSI expression was observed in all floral whorls at early developmental stages, but it was restricted to carpel before anthesis. These results give new insights into the role of starch in later reproductive events such as seed filling. In leaves the mRNA level of GBSSI is regulated by an endogenous circadian clock, indicating that the transition from day to night may be accompanied by abolition of expression of starch synthesis genes. This mechanism does not operate in sink tissues such as roots when grown in the dark.

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“…It has been reported that the expression of starch synthesis genes, such as starch synthase in potato ; ADP-glucose pyrophosphorylase in potato (Müller-Röber et al, 1990), sweet potato (Bae and Liu, 1997), Arabidopsis (Rook et al, 2001), and tomato (Li et al, 2002); SBE in potato (Kossman et al, 1991), maize (Kim and Guiltinan, 1999), and Arabidopsis (Khoshnoodi et al, 1998); and isoamylase in barley , is sugar inducible. In contrast to the situation in bacteria, yeast, and mammals, in which sugar signaling cascades have been studied extensively, the sugar signaling transduction pathways in plants are poorly understood (Rolland et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

A Novel WRKY Transcription Factor, SUSIBA2, Participates in Sugar Signaling in Barley by Binding to the Sugar-Responsive Elements of theiso1Promoter [W]

et al. 2003

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SURE (sugar responsive) is a cis element in plant sugar signaling. The SURE element was reported first for potato, in which it confers sugar responsiveness to the patatin promoter. A SURE binding transcription factor has not been isolated. We have isolated a transcription factor cDNA from barley and purified the corresponding protein. The transcription factor, SUSIBA2 (sugar signaling in barley), belongs to the WRKY proteins and was shown to bind to SURE and W-box elements but not to the SP8a element in the iso1 promoter. Nuclear localization of SUSIBA2 was demonstrated in a transient assay system with a SUSIBA2:green fluorescent protein fusion protein. Exploiting the novel transcription factor oligodeoxynucleotide decoy strategy with transformed barley endosperm provided experimental evidence for the importance of the SURE elements in iso1 transcription. Antibodies against SUSIBA2 were produced, and the expression pattern for susiba2 was determined at the RNA and protein levels. It was found that susiba2 is expressed in endosperm but not in leaves. Transcription of susiba2 is sugar inducible, and ectopic susiba2 expression was obtained in sugar-treated leaves. Likewise, binding to SURE elements was observed for nuclear extracts from sugar-treated but not from control barley leaves. The temporal expression of susiba2 in barley endosperm followed that of iso1 and endogenous sucrose levels, with a peak at ‫ف‬ 12 days after pollination. Our data indicate that SUSIBA2 binds to the SURE elements in the barley iso1 promoter as an activator. Furthermore, they show that SUSIBA2 is a regulatory transcription factor in starch synthesis and demonstrate the involvement of a WRKY protein in carbohydrate anabolism. Orthologs to SUSIBA2 were isolated from rice and wheat endosperm.

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Differential accumulation of Arabidopsis thaliana Sbe2.1 and Sbe2.2 transcripts in response to light

Cited by 11 publications

References 33 publications

Impaired sucrose‐induction mutants reveal the modulation of sugar‐induced starch biosynthetic gene expression by abscisic acid signalling

Impaired sucrose‐induction mutants reveal the modulation of sugar‐induced starch biosynthetic gene expression by abscisic acid signalling

Expression of the Granule-Bound Starch Synthase I (Waxy) Gene from Snapdragon Is Developmentally and Circadian Clock Regulated1

A Novel WRKY Transcription Factor, SUSIBA2, Participates in Sugar Signaling in Barley by Binding to the Sugar-Responsive Elements of theiso1Promoter [W]

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