Different γ/δ T clones sustain GVM and GVH effects in multiple myeloma patients after non-myeloablative transplantation

Galimberti, Sara; Benedetti, Enrico; Morabito, Fortunato; Petrini, Iacopo; Battolla, Barbara; Papineschi, Federico; Fazzi, Rita; Ciabatti, Elena; Martino, Massimo; Cuzzola, Mariella; Console, Giuseppe; Iacopino, Pasquale; Petrini, Mario

doi:10.1016/j.leukres.2005.09.004

Cited by 15 publications

(15 citation statements)

References 19 publications

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“…Regarding treatment protocols, the temporary restriction of the γδ TCR gene repertoire after allogeneic HSC transplantation (Hirokawa et al. , 2000) as well as the monoclonality and the oligoclonality of Vδ gene families in relation to acute graft‐versus‐host diseases and the eradication of minimal residual disease (Galimberti et al. , 2006) were observed.…”

Section: Discussionmentioning

confidence: 99%

“…With cancer progression, this Vc2Vd2 T cell tumour surveillance gradually decreases because of immunologic escape of tumour cells and prolonged immunosuppression caused by the disease itself and common treatment regimens (Mariani et al, 2005;Kiladjian et al, 2008;Kunzmann et al, 2009). Regarding treatment protocols, the temporary restriction of the cd TCR gene repertoire after allogeneic HSC transplantation (Hirokawa et al, 2000) as well as the monoclonality and the oligoclonality of Vd gene families in relation to acute graft-versus-host diseases and the eradication of minimal residual disease (Galimberti et al, 2006) were observed.…”

Section: Discussionmentioning

confidence: 99%

“…However, in autologous conditions, the contribution of Vd2 T cells to minimal residual disease elimination is not well understood. On the contrary, after allogeneic transplantation Vd2 T-cell clones were found to have a major role in the achievement of minimal residual disease negativity in multiple myeloma (Galimberti et al, 2006). The patient group in our current study was used primarily to demonstrate the established methodology.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequent gel electrophoresis and Southern blot analysis enables to qualitatively evaluate the presence of individual gene families (Olive et al, 1992(Olive et al, , 1994Bucht et al, 1995). Exact PCR fragment sizing, termed CDR3 spectratyping, identifies the clonality of the analysed families (Shen et al, 1998;Hirokawa et al, 2000;Galimberti et al, 2006). To examine TCR diversity quantitatively, real-time PCR and oligonucleotide microarrays were established to analyse the relative expression of individual gene families in the ab T-cell population (Brewer & Ericson, 2005;Chen et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Relative expression of γδ T‐cell receptor gene families detected by RT–PCR and capillary electrophoresis

Tesařová

Koutná

Dvoráková

et al. 2012

Int J Immunogenetics

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Summary γδ T cells are intensively studied because their function in infection, allergy, autoimmune disease, cancer and post‐transplant period is not yet fully understood. PCR‐based techniques were established to study the γ variable (Vγ) and δ variable (Vδ) gene families. PCR product evaluation is routinely carried out by Southern blot analysis or the third complementarity‐determining region spectratyping, but a fast and simple assessment of Vγ and Vδ gene family expression is missing. The aim of our study was to test capillary electrophoresis as a potential method for evaluating the composition of the γδ T‐cell population. This report provides optimized PCR conditions for γδ T‐cell receptor amplification. Further, it describes the utilization of capillary electrophoresis in the Agilent 2100 Bioanalyzer to evaluate the relative expression of Vγ and Vδ gene families after their amplification. An application of the methodology to peripheral blood mononuclear cell samples from patients during haemato‐oncological treatment is shown. The described methodology is fast and simple to operate and is therefore suitable as a first screening of the γδ T‐cell population composition in tissues of interest.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Relative expression of γδ T‐cell receptor gene families detected by RT–PCR and capillary electrophoresis

Tesařová

Koutná

Dvoráková

et al. 2012

Int J Immunogenetics

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“…This increased effector activity was dependent on IPP accumulation and TCR-mediated recognition of tumor cells. Vc9Vd2 T cells have recently been included among the immune effector cells playing a major role in the achievement of MRD negativity after non-myeloablative allogeneic transplantation in MM [78]. In seven of the nine patients who became MRD negative, the appearance of a new predominant Vc9Vd2 T cell clone was concomitant with the disappearance of the tumor cell clone.…”

Section: Future Directionsmentioning

confidence: 99%

Vγ9Vδ2 T cell-based immunotherapy in hematological malignancies: from bench to bedside

Castella

Vitale

Coscia

et al. 2011

Cell. Mol. Life Sci.

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Many hematological malignancies consist of tumor cells that are spontaneously recognized and killed by Vγ9Vδ2 T cells. These tumor cells generate high amounts of intracellular phosphorylated metabolites mimicking the natural ligands and display a wide range of stress-induced self-ligands that are recognized by Vγ9Vδ2 T cells via TCR-dependent and TCR-independent mechanisms. The intrinsic features of Vγ9Vδ2 T cells and that of tumor cells of hematological origin constitute an ideal combination from which to develop Vγ9Vδ2 T cell-based immune interventions. In this review, we will discuss the rationale, preclinical and clinical data in favor of this therapeutic strategy and the future perspectives of its development.

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Current Awareness in Hematological Oncology

2006

Hematological Oncology

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of hematological oncology. Each bibliography is divided into 14 sections: 1 Books, Reviews & Symposia; 2 General; Leukemias: 3 Lymphoblastic; 4 Myeloid & Myelodysplastic Syndromes; 5 Chronic; 6 Others; Lymphomas: 7 Hodgkin's; 8 Non‐Hodgkin's; 9 Plasmacytomas/Multiple Myelomas; 10 Others; 11 Bone Marrow Transplantation; 12 Cytokines; 13 Diagnosis; 14 Cytogenetics. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted.

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Different γ/δ T clones sustain GVM and GVH effects in multiple myeloma patients after non-myeloablative transplantation

Cited by 15 publications

References 19 publications

Relative expression of γδ T‐cell receptor gene families detected by RT–PCR and capillary electrophoresis

Relative expression of γδ T‐cell receptor gene families detected by RT–PCR and capillary electrophoresis

Vγ9Vδ2 T cell-based immunotherapy in hematological malignancies: from bench to bedside

Current Awareness in Hematological Oncology

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