Different vehicles containing CaneCPI-5 reduce erosive dentin wear in situ

Pelá, Vinícius Taioqui; Gironda, Carlos Condarco; Taira, Éven Akemi; Brito, Leonardo; Pieretti, Joana C.; Seabra, Amedea B.; Cardoso, Cristiane de Almeida Baldini; Rodrigues, Marcela Charantola; Henrique-Silva, Flávio; Buzalaf, Marília Afonso Rabelo

doi:10.1007/s00784-023-05175-z

Cited by 1 publication

(2 citation statements)

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“…Ten volunteers (4 males and 6 females, age range [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] participated in this in vivo study. The volunteers met the following inclusion criteria: good health, both oral and general (could not present gingivitis, dental caries, periodontal disease, or any oral cavity changes that might alter oral fluids' composition), absence of restorations on the buccal surfaces of all upper and lower teeth, non-smokers, and not on medication that might decrease saliva flow.…”

Section: Ethical Aspects and Subjectsmentioning

confidence: 99%

“…Thus, future studies should focus on protocols involving prolonged erosive challenges combined with abrasion by toothbrushing. Solutions [19] and chitosan gels [34] containing CaneCPI-5 have been shown to be more effective than placebo and as effective as commercial stannous mouthwash or NaF gel in preventing prolonged erosion (citric acid) combined with abrasion in situ. Similar results were observed for StN15 for erosive challenges conducted with HCl (unpublished data).…”

Section: Hemoglobin Reduces Intrinsic Erosionmentioning

confidence: 99%

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Hemoglobin Protects Enamel against Intrinsic Enamel Erosive Demineralization

Carvalho,

Araújo,

Ventura

et al. 2024

Caries Res

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Introduction: This study investigated the changes in the acquired enamel pellicle (AEP) proteome when this integument is formed in vivo after treatment with sugarcane-derived cystatin (CaneCPI-5), hemoglobin (HB), and a statherin-derived peptide (StN15), or their combination and then exposed to an intrinsic acid challenge. The effectiveness of these treatments in preventing intrinsic erosion was also evaluated. Methods: Ten volunteers, after prophylaxis, in 5 crossover phases, rinsed with the following solutions (10mL, 1min): control (deionized water-H2O)-group 1, 0.1mg/mL CaneCPI-5- group 2, 1.0mg/mL HB- group 3, 1.88x10-5M StN15- group 4 or a blend of these- group 5. Following this, AEP formation occurred (2h) and an enamel biopsy (10µL, 0.01M HCl, pH 2.0, 10s) was conducted on one incisor. The biopsy acid was then analyzed for calcium (Arsenazo method). The vestibular surfaces of the other teeth were treated with the same acid. Acid-resistant proteins in the residual AEP were then collected and analyzed quantitatively via proteomics. Results: Compared to control, treatment with the proteins/peptide, mixed or isolated, markedly enhanced acid-resistant proteins in the AEP. Notable increases occurred in pyruvate kinase PKM (11-fold, CaneCPI-5), immunoglobulins and submaxillary gland androgen-regulated protein 3B (4-fold, StN15), Hb and lysozyme-C (2-fold, StN15). Additionally, a range of proteins not commonly identified in the AEP, but known to bind calcium or other proteins were in groups treated with the tested proteins/peptide either in isolation or as a mixture. The mean (SD, mM) calcium concentrations released from enamel were 3.67±1.48a, 3.11±0.72a, 1.94±0.57b, 2.37±0.90a and 2.38±0.45a for groups 1-5, respectively (RM-ANOVA/Tukey, p<0.05). Conclusions: Our findings demonstrate that all treatments, whether using a combination of proteins/peptides or in isolation, enhanced acid-resistant proteins in the AEP. However, only HB showed effectiveness in protecting against intrinsic erosive demineralization. These results pave the way for innovative preventive methods against intrinsic erosion, using "acquired pellicle engineering" techniques.

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