Different urea stoichiometries between the dissociation and denaturation of tobacco mosaic virus as probed by hydrostatic pressure

Santos, Jose L.R.; Aparício, Ricardo; Joekes, Inés; Silva, Jerson L.; Bispo, José Ailton Conceição; Bonafé, Carlos Francisco Sampaio

doi:10.1016/j.bpc.2008.02.010

Cited by 20 publications

(33 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pressure-induced denaturation has been widely applied in virology. Whereas the primary structure of proteins is unchanged at pressures as high as 10 kbar, dissociation is nevertheless quite frequently observed (31,32,50,51). In general, the effect of pressure on a physicochemical process at equilibrium is governed by the volume change of the process, DV (31,32).…”

Section: Discussionmentioning

confidence: 99%

“…Hence, the application of pressure will change the balance in favor of the state with the lowest overall volume. The magnitude of the changes in volume in the structure of a given protein will depend on specific molecular interactions (9,50,51). Because of its ability to reversibly dissociate oligomeric proteins, HHP has been increasingly used as an alternative method for viral inactivation in vaccine production.…”

Section: Discussionmentioning

confidence: 99%

“…At higher pressures, nonspecific aggregates of capsid proteins predominate over seemingly complete reassociated capsids. The formation of imperfect virus particles after a cycle of compression and decompression has also been demonstrated for human norovirus (49) and rotavirus (41,46) by electron microscopy, gel filtration, and spectroscopy (8,50,51,53). In this article, we describe the effects of pressure and chemical treatments on TMV by mapping the linear epitopes through spot synthesis and comparing them to results obtained with epitope prediction software.…”

mentioning

confidence: 99%

“…Observations of comparable effects on multi-subunit proteins and viruses have led to the idea of using hydrostatic pressure to suppress virus infectivity, while preserving or perhaps improving the immunogenic properties. In most cases, effective immunization against viruses requires presentation of the whole virus particle to the immune system (9,50,51). This requirement, coupled to the need to eliminate infectivity, greatly limits the possibilities of preparing appropriate vaccines.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Influence of High Hydrostatic Pressure on Epitope Mapping of Tobacco Mosaic Virus Coat Protein

Neto

Sampaio²,

Arns³

2014

Viral Immunology

View full text Add to dashboard Cite

In this study, we investigated the effect of high hydrostatic pressure (HHP) on tobacco mosaic virus (TMV), a model virus in immunology and one of the most studied viruses to date. Exposure to HHP significantly altered the recognition epitopes when compared to sera from mice immunized with native virus. These alterations were studied further by combining HHP with urea or low temperature and then inoculating the altered virions into Balb-C mice. The antibody titers and cross-reactivity of the resulting sera were determined by ELISA. The antigenicity of the viral particles was maintained, as assessed by using polyclonal antibodies against native virus. The antigenicity of canonical epitopes was maintained, although binding intensities varied among the treatments. The patterns of recognition determined by epitope mapping were cross checked with the prediction algorithms for the TMVcp amino acid sequence to infer which alterations had occurred. These findings suggest that different cleavage sites were exposed after the treatments and this was confirmed by epitope mapping using sera from mice immunized with virus previously exposed to HHP.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Influence of High Hydrostatic Pressure on Epitope Mapping of Tobacco Mosaic Virus Coat Protein

Neto

Sampaio²,

Arns³

2014

Viral Immunology

View full text Add to dashboard Cite

show abstract

“…Bonafe et al (1998) showed how the apparent free energy associated with denaturation by urea for TMV protein coat was very low, almost null. The apparent urea stoichiometry of dissociation and denaturation of further viral agents has been calculated (Santos et al, 2008).…”

Section: Action Mechanism Of Synthetic Productsmentioning

confidence: 99%

Chemical Outbreak for Tobacco Mosaic Virus Control

Luvisi¹,

Panattoni²,

Materazzi³

et al. 2017

IJAB

View full text Add to dashboard Cite

Tobacco mosaic virus (TMV) represents a paradigm in virology, and its control may open the way to effective treatment against phytoviruses. However, the use of chemicals to eliminate the virus from infected plants is very difficult. In this state of the art survey we include trials on a) natural compounds derived from organisms, b) synthetic compounds and c) plant or microorganism extracts, from 2006 to 2015. Plants have been the main source of natural products for anti-TMV tests in the last ten years, and Nicotiana tabacum was the main focus of research, particularly between 2014-2015. Since 2012, there has been a great increase in publications (+45%) and identified compounds (+241%). Between 2012-2015, an average of 31 papers were published and 140 compounds were tested each year, compared to 9 papers and 26 compounds in 2006-2011. Unfortunately, there is little information on the action mechanisms of newly discovered or modified compounds. Cross references to the basic structure of compounds is provided in this review. This chemical outbreak this massive interest in chemical solutions to TMV could be due to the increasing availability of instruments for the analysis of organic compounds. Alternatively Another explanation could be that the chemistry advances in synthesis, which have provided countless drugs with potential benefits for TMV control, have overwhelmed overloaded the plant pathology screening needed to discriminate between compounds and to provide useful agrochemicals for farmers.

show abstract

Thermodynamic and kinetic modeling of the protection of mammalian cells against ethanol‐induced damage by extracts of Jatropha curcas (Euphorbiaceae)

Cardoso

Esteves

Bonafé

et al. 2019

Engineering Reports

View full text Add to dashboard Cite

The medicinal plant Jatropha curcas exerts beneficial effects against ethanol toxicity in humans. In this work, we investigated the protective effect of three extracts (ethanol [Et], vegetal glycerin [VG], and propylene glycol [PG]) of J. curcas leafs against Et‐induced damage in human peripheral blood mononuclear cells. The cytotoxicity of the extracts alone or in combination with each other in mononuclear cells was also assessed. A thermodynamic and kinetic analysis of the protection assumed that the cells were in a metastable equilibrium of growth and death, and the mass action of the extracts was used to determine the optimal concentration for each extract. The PG and VG extracts were cytotoxic, with an efficiency index of protection (η) of approximately −20% and −41%, respectively. By contrast, η values of ∼220% were observed for Et extracts (0.625% vol/vol) and combined extracts (Et + PG) at concentrations of (0.375 + 0.250)% and (0.5 + 0.5)%, respectively. These results identify J. curcas as a promising source of bioactive compounds and show that appropriate modeling can provide a powerful system for determining the optimal conditions for drug action.

show abstract

Different urea stoichiometries between the dissociation and denaturation of tobacco mosaic virus as probed by hydrostatic pressure

Cited by 20 publications

References 42 publications

Influence of High Hydrostatic Pressure on Epitope Mapping of Tobacco Mosaic Virus Coat Protein

Influence of High Hydrostatic Pressure on Epitope Mapping of Tobacco Mosaic Virus Coat Protein

Chemical Outbreak for Tobacco Mosaic Virus Control

Thermodynamic and kinetic modeling of the protection of mammalian cells against ethanol‐induced damage by extracts of Jatropha curcas (Euphorbiaceae)

Contact Info

Product

Resources

About