Different Types of Non-Starch Polysaccharides Alter the Growth, Intestinal Flora and Serum Metabolite Profile of Grass Carp, Ctenopharyngodon idella

Liu, Yu; Fu, Xinlangji; Zhou, Hang; Fan, Jiongting; Huang, Huajing; Deng, Junming; Tan, Beiping

doi:10.3390/metabo12101003

Cited by 4 publications

(2 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Insoluble ASP was superior to water-soluble WSP and CMP in increasing the richness and diversity of intestinal microorganisms. It has also been found that insoluble dietary fiber (IDF) is better than soluble dietary fiber (SDF) in alleviating colitis in mice by regulating gut microbiota [33] , and the addition of dietary insoluble polysaccharides can establish a health microbial community, which is conducive to intestinal health [34] . In this study, ASP and its modified product CMP showed stronger effects of promoting the proliferation of probiotics and inhibiting the growth of harmful bacteria than that of WSP.…”

Section: Discussionmentioning

confidence: 99%

Adsorption, in vitro digestion and human gut microbiota regulation characteristics of three Poria cocos polysaccharides

Zhang,

Zheng,

Zheng

et al. 2024

Food Science and Human Wellness

View full text Add to dashboard Cite

Poria cocos (PC) is a famous traditional Chinese medicine (TCM) and a widely used healthcare ingredient, which has antiobesity, enhancing immunity and improving sleep effects. Traditionally, only water-soluble poria polysaccharide (WSP) is extracted and applied for clinical application, while insoluble polysaccharide (alkali-soluble poria polysaccharide, ASP) is discarded as herb residue. However, the whole PC has also been historically utilized as functional herbal food. Considering the benefi cial role of dietary fi ber and the traditional use of PC, ASP may also contribute substantially to the therapy function of PC. Compared to WSP, little attention has been paid to ASP and ASP modifi ed product carboxymethyl poria polysaccharide (CMP) which has been used as an antitumor adjuvant drug. In this study, the oil, cholesterol, metal ions and polyphenols adsorption ability, in vitro simulated digestive and the gut microbiota fermentation characteristics of WSP, ASP and CMP were studied to evaluate the functional values of three P. cocos polysaccharides (PCPs). The results showed that all three PCPs had good adsorption capacity on cholesterol, polyphenols and metal ions (Cd 2+ /Zn 2+ /Mg 2+ ), among which ASP showed the highest capacity than WSP and CMP. The adsorption capacity of all three PCPs on heavy metal ions (Cd 2+ /Zn 2+ ) was stronger than that of non-heavy metal ions (Mg 2+ ); The in vitro digestibility of all three PCPs was very low, but WSP was slightly higher than ASP and CMP; Moreover, the indigestible residue of all three PCPs could improve the richness and diversity of gut microbiota, among which ASP had the greatest infl uence. In general, ASP and CMP could signifi cantly promote the proliferation of some probiotics and inhibit the growth of some harmful bacteria. The gut microbiota diversity of CMP was reduced, but the richness of probiotics, especially Parabacteroides distasonis was significantly enhanced compared with the ASP group, and the growth of harmful bacteria Klebsiella pneumoniae was inhibited after CMP treatment. The short-chain fatty acids (SCFAs) analysis results showed that all three PCPs could signifi cantly promote the production of acetic acid, propionic acid and the total acid content compared with blank control group, and SCFAs producing activity was positively correlated with the proliferative capacity of probiotics. Taken together, the good adsorption characteristics and gut microbiota regulatory activity of ASP may lay foundation for its lipid-lowering and immune-improving function. Additionally, the probiotic effect of CMP and ASP indicated that except for only use the water extract of PC in clinic, CMP and ASP also can be used in healthcare to take full advantage of this valuable medicine.

show abstract

Section: Discussionmentioning

confidence: 99%

Adsorption, in vitro digestion and human gut microbiota regulation characteristics of three Poria cocos polysaccharides

Zhang,

Zheng,

Zheng

et al. 2024

Food Science and Human Wellness

View full text Add to dashboard Cite

show abstract

“…Bacteria DNA was extracted by a commercial kit using the SDS method (Magen, Guangzhou, China), following a quality and purity test. Subsequently, the DNA samples were amplified and purified according to the formulation of Liu et al [22], followed by library construction and sequencing of the cDNA. Sequencing work was carried out by Hiseq2500 PE250 platform (Illumina, San Diego, CA, USA), and sequencing data analysis work was carried out by Novogene Co., Ltd. (Beijing, China).…”

Section: Microbiome Analysismentioning

confidence: 99%

Feeding Rainbow Trout with Different Types of Non-Starch Polysaccharides: Impacts on Serum Metabolome and Gut Microbiota

et al. 2022

Self Cite

View full text Add to dashboard Cite

A 70-day feeding trial investigated the effects of dietary inclusion of different types of non-starch polysaccharides (NSPs) on gut microbiota and serum metabolome of rainbow trout. Four practical feeds (42% crude protein, 17% crude lipid) were prepared with 8% insoluble NSP (INSP, cellulose), 16.8% soluble NSP (SNSP, composed of 1.12% β-glucan, 1.28% mannan, 4.8% arabinoxylan, and 9.6% pectin), 24.8% NSPs (8% INSP + 16.8% SNSP), or no NSPs inclusion, respectively. Dietary NSPs inclusion had no significant influence on the Shannon, Simpson, ACE, and Chao1 indices of gut microbiota but induced a significant increase in the abundance of Pseudomonas aeruginosa and Photobacterium kishitanii, and a decrease in Firmicutes and Alistipes finegoldii. Besides, dietary SNSP upregulated the carnitine synthesis metabolic pathway. Our data suggest that dietary NSPs are detrimental to gut microbiota homeostasis and the health of rainbow trout, and dietary SNSP exhibit a stronger ability to interfere with physiological metabolism of rainbow trout than INSP. Therefore, the physiological effects of dietary NSPs, especially SNSP, should be carefully considered when designing the commercial feed formulations of rainbow trout.

show abstract

Supplementing the Diet of Hybrid Grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) with Compound Acidifier Is a Good Way to Alleviate Poor Intestinal Growth Caused by High Levels of Cottonseed Protein Concentrate

Zhang,

Deng,

Liang

et al. 2024

Aquaculture Nutrition

View full text Add to dashboard Cite

This study investigated the effect of adding the compound acidifiers on the intestine health of hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) fed diet with higher cottonseed protein concentrate (CPC). To assess the benefits of compound acidifiers helping CPC to replace more fish meal (FM) for hybrid grouper, five isonitrogen and isolipid experimental diets were formulated. The five diets consisted of a control diet (FM, 50%) and four experimental diets (27.5% FM, 27.27% CPC), each of which contained compound acidifiers to varying degrees (FCA0, FCA0.05, FCA0.1, and FCA0.2). Each diet was fed to three replicates of each group for 8 weeks. In the FCA0.05 group, the results demonstrated a statistically significant increase in protein efficiency ratio, lipid efficiency ratio, and the activity of digestive enzymes in the intestine (P < 0.05). In the FCA0.05 group, there was a clearly enhanced expression of anti‐inflammatory cytokines in the intestines (P < 0.05). In the FCA0.1 group, there may be an increase in the expression of important genes involved in fatty acid metabolism in the intestinal (P < 0.05). Appropriate compound acidifiers decreased the relative abundance of Vibrio and other harmful bacteria in the intestine flora that were brought by high CPC. In conclusion, when the hybrid grouper was given a diet containing more CPC, the FCA0.05 group was able to promote intestinal development, enhance digestive enzyme activity and feed consumption, raise antioxidant enzyme activity and anti‐inflammatory factor expression, and improve intestinal health.

show abstract

Different Types of Non-Starch Polysaccharides Alter the Growth, Intestinal Flora and Serum Metabolite Profile of Grass Carp, Ctenopharyngodon idella

Cited by 4 publications

References 86 publications

Adsorption, in vitro digestion and human gut microbiota regulation characteristics of three Poria cocos polysaccharides

Adsorption, in vitro digestion and human gut microbiota regulation characteristics of three Poria cocos polysaccharides

Feeding Rainbow Trout with Different Types of Non-Starch Polysaccharides: Impacts on Serum Metabolome and Gut Microbiota

Supplementing the Diet of Hybrid Grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂) with Compound Acidifier Is a Good Way to Alleviate Poor Intestinal Growth Caused by High Levels of Cottonseed Protein Concentrate

Contact Info

Product

Resources

About