Different types of degradable vectors from low-molecular-weight polycation-functionalized poly(aspartic acid) for efficient gene delivery

Dou, Xuebo; Hu, Yang; Zhao, Nana; Xu, Fu‐Jian

doi:10.1016/j.biomaterials.2013.12.017

Cited by 40 publications

(49 citation statements)

References 35 publications

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“…Such responsiveness can be evaluated by agarose gel retardation electrophoresis. 32 As shown in Figure 3(k) and 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 15 those of their corresponding star CD-PAsp-DET/pDNA complexes at the same N/P ratio. These results corresponded to their different DNA-condensing ability (Figure 3).…”

Section: Self-assembly Between Pasp-ea-bla and Cd-pasp-det Supramolementioning

confidence: 97%

“…35 The detailed procedures were described earlier. 32 The stained cells were imaged using a Leica 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 10 Technologies GmbH. KG, Bad Wildbad, Germany).…”

Section: Self-assembly Between Pasp-ea-bla and Cd-pasp-det Supramolementioning

confidence: 99%

“…Details on the preparation and characterization of β-CD-NH 2 with four primary amine groups were described in our previous work. 32 to produce about 0.55 g of α-CD-NH 2 .…”

Section: Introductionmentioning

confidence: 99%

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Poly(aspartic acid)-based Degradable Assemblies for Highly Efficient Gene Delivery

Nie

Dou

et al. 2014

ACS Appl. Mater. Interfaces

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Due to its good properties such as low cytotoxicity, degradability, and biocompatibility, poly(aspartic acid) (PAsp) is a good candidate for the development of new drug delivery systems. In this work, a series of new PAsp-based degradable supramolecular assemblies were prepared for effective gene therapy via the host-guest interactions between the cyclodextrin (CD)-cored PAsp-based polycations and the pendant benzene group-containing PAsp backbones. Such supramolecular assemblies exhibited good degradability, enhanced pDNA condensation ability, and low cytotoxicity. More importantly, the gene transfection efficiencies of supramolecular assemblies were much higher than those of CD-cored PAsp-based counterparts at various N/P ratios. In addition, the effective antitumor ability of assemblies was demonstrated with a suicide gene therapy system. The present study would provide a new means to produce degradable supramolecular drug delivery systems.

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Section: Self-assembly Between Pasp-ea-bla and Cd-pasp-det Supramolementioning

confidence: 97%

Section: Self-assembly Between Pasp-ea-bla and Cd-pasp-det Supramolementioning

confidence: 99%

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Poly(aspartic acid)-based Degradable Assemblies for Highly Efficient Gene Delivery

Nie

Dou

et al. 2014

ACS Appl. Mater. Interfaces

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“…The cul-254 tures were maintained at 37°C in a humidified atmosphere con-255 taining 5% CO 2 for 7 days in vitro (DIV) prior to experimentation. 256 The amount of astrocytes, as determined by the content of [7]. The products of these reactions are 361 referred to in the text as GCD-GTMAC and GCD-EDA, respectively.…”

mentioning

confidence: 99%

Enhanced delivery of daidzein into fibroblasts and neuronal cells with cationic derivatives of gamma-cyclodextrin for the control of cellular glycosaminoglycans

Kamiński

Kujdowicz

Kajta

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

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“…10,11 Polyethylenimine, polyamidoamine, poly(L-lysine) and poly [2-(dimethylamino)ethyl methacrylate] have all drawn extensive attraction as non-viral gene delivery carriers. [12][13][14][15][16] This chapter only focuses on poly(L-lysine) and polyallylamine by summarizing the recent developments of their effects on the functions of stem cells and highlighting the challenges of usage of such cationic polymers for biomedical applications.…”

mentioning

confidence: 99%

Effects of Cationic Polymers on Cell Functions

Chen

2014

Cationic Polymers in Regenerative Medicine

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Cells are surrounded in vivo by a specific microenvironment that is composed of different neighboring cells and various types of cytokines, growth factors and extracellular matrices (ECMs) with diverse electrostatic properties. The microenvironment provides the necessary biological and physicochemical cues to control cell functions and metabolism. 1 The interaction between cells and the microenvironment has been well studied to elucidate the effect of the microenvironment on cell functions such as adhesion, proliferation and differentiation. 2,3 Cells interact with the microenvironment through not only cell-cell interactions and receptor-ligand interactions, but also electrostatic interactions because of the diversity of the electrostatic properties of the ECM. The chargeable ECM components affect cell adhesion and functions by direct mediation of cell-ECM interaction or through adjusting the diffusion and distribution of small molecules such as ions, cytokines and growth factors. 4,5 To mimic the microenvironment, chargeable polymers with different electrostatic properties have been used to study their effects on cell functions for various applications. For example, RSC Polymer Chemistry Series No. 13 Cationic Polymers in Regenerative Medicine Edited by Sangram K. Samal and Peter Dubruel

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Different types of degradable vectors from low-molecular-weight polycation-functionalized poly(aspartic acid) for efficient gene delivery

Cited by 40 publications

References 35 publications

Poly(aspartic acid)-based Degradable Assemblies for Highly Efficient Gene Delivery

Poly(aspartic acid)-based Degradable Assemblies for Highly Efficient Gene Delivery

Enhanced delivery of daidzein into fibroblasts and neuronal cells with cationic derivatives of gamma-cyclodextrin for the control of cellular glycosaminoglycans

Effects of Cationic Polymers on Cell Functions

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