Different Types and Intense Classification of 2D Materials

Garg, Mayank; Vishwakarma, Neelam; Sharma, Amit L.; Singh, Suman

doi:10.1007/978-981-16-3322-5_2

Cited by 3 publications

(2 citation statements)

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“…To test the efficacy of the fluorescent probe (Ab@fGQDs) in both real samples, serum was spiked with six different hTERT-Ag concentrations (3,7,30,70,150 and 300 ng ml −1 ) and the PL was measured. Surprisingly, increasing hTERT-Ag concentration resulted in a recovery in fluorescence intensity.…”

Section: Real Sample Analysismentioning

confidence: 99%

“…The emergence of carbon-based fluorescent nanomaterials has recently boosted bioanalytical and biosensing methodologies. Among these materials, carbon dots (CDs) [24] and graphene quantum dots (GQDs) are two well-known novel fluorescent materials that have surprised the research fraternity due to their broad applications [25][26][27][28] GQDs, in particular, have sparked interest in theranostics and bioanalytical applications due to their exceptional photophysical properties, surface tunable features and biocompatibility [25,29,30].…”

Section: Introductionmentioning

confidence: 99%

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Biofabricated functionalized graphene quantum dots (fGQDs): unraveling its fluorescence sensing mechanism of human telomerase reverse transcriptase (hTERT) antigen and in vitro bioimaging application

Tade¹,

Patil²

2022

Biomed. Mater.

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Lung cancer (LC) is a deadly malignancy that is posing a serious threat to human health. Therefore, early detection of LC biomarkers is the key to reducing LC-related fatalities. Herein, we present the first fluorescent-based selective detection of LC biomarker human telomerase reverse transcriptase (hTERT) using polyethyleneimine (PEI) functionalized graphene quantum dots (fGQDs). One-pot in situ synthesis of amine-functionalized GQDs was accomplished by hydrothermal carbonization of biowaste-derived cellulose and PEI. Synthesized fGQDs were characterized by various analytical techniques. Synthesized fGQDs not only exhibited enhanced fluorescence life-time but also excellent stability in the different solvents compared to bare GQDs. The surface activation of hTERT-Ab by carbodiimide chemistry (EDC-NHS) resulted in stacking interactions with fGQDs, involving adsorption-desorption as well as competitive mechanisms. The higher inherent affinity of hTERT-Ag (hTERT antigen) for hTERT-Ab (hTERT antibody) resulted in complex formation and recovery of fGQD fluorescence. As a result, this fluorescence sensing demonstrated a greater linear detection range (0.01 ng mL-1 to 100 µg mL-1) as well as a notable low detection limit (36.3 pg mL-1). Furthermore, the fabricated immunosensor (Ab@fGQDs) has excellent stability and performance in real samples, with an average recovery of 97.32%. The results of cytotoxicity and cellular bioimaging study in A549 cells show that fGQDs can be used for additional nanotherapeutics and biological applications.

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Section: Real Sample Analysismentioning

confidence: 99%