Different toxic, fibrogenic and mutagenic effects of four commercial quartz flours in the rat lung

Seiler, Frank; Rehn, B.; Rehn, Steffeni; Bruch, Joachim

doi:10.1078/1438-4639-00275

Cited by 24 publications

(24 citation statements)

References 21 publications

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“…More recently, Fubini et al (2004) characterized four different quartz samples and could attribute physicochemical properties to the varying lung toxicity potential observed by Seiler et al (2004) in parallel rat studies. Two samples induced inflammation in vivo, whereas the other two were mostly inert.…”

Section: Discussionmentioning

confidence: 99%

Toxicity of a Quartz with Occluded Surfaces in a 90-Day Intratracheal Instillation Study in Rats

Creutzenberg

Hansen

Ernst

et al. 2008

Inhalation Toxicology

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Section: Discussionmentioning

confidence: 99%

Toxicity of a Quartz with Occluded Surfaces in a 90-Day Intratracheal Instillation Study in Rats

Creutzenberg

Hansen

Ernst

et al. 2008

Inhalation Toxicology

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“…Intratracheal instillation of α-quartz (Min-U-Sil 5) in rats induced apoptosis in both alveolar and granulomatous cells (Leigh et al, 1997). The ability of crystalline silica to induce pulmonary injury depends not only on length and amount of exposure but also from the type of quartz used (source, presence of metal impurity, particle size) and from the preparation modality (fresh or aged), which infl uences the biological activity (Donaldson and Borm 1998;Fubini et al, 2001;Schins et al, 2002;Seiler et al, 2004). The biological eff ects of crystalline silica are principally related to surface characteristics and reactivity, which determine the toxicological, oxidant and genotoxic properties of crystalline silica.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of cytotoxic concentration–time response in A549 cells exposed to respirable α‐quartz

Fanizza¹,

Fresegna

Maiello

et al. 2009

J of Applied Toxicology

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A causal pathway between quartz, silicosis and lung cancer has been postulated. The aim of our study was to assess cytotoxic effects induced in a human lung epithelial cell line (A549) by exposure to alpha-quartz. Cells were exposed to respirable alpha-quartz (SRM1878a, NIST) at 25, 50 or 100 microg ml(-1 )for 24 h and at 50 or 100 microg ml(-1) for 48 h. Cytotoxic effects were analyzed by scanning electron microscopy (SEM), apoptotic morphology analysis with Hoechst staining and lactate dehydrogenase (LDH) release assay. In cells exposed to alpha-quartz for 24 h, a concentration-dependent bleb development and in particular the localization of blebs at the cell edge at higher concentrations were observed. The blebbing phenomenon was more evident after 48 h of exposure to 50 or to 100 microg ml(-1) of alpha-quartz and large blebs were localized at the cell edge. At the same concentrations surface smoothing was also observed. Moreover the presence of holes and tears was detected at the highest concentration both at 24 and 48 h. Results of morphological analysis with Hoechst stain evidenced an increase concentration-time dependent of apoptotic cell percentage that was more marked after 48 h exposure to 100 microg ml(-1) and a prevalence of late apoptosis stage with the increase of exposure time and concentration. Cells exposed to 50 or 100 microg ml(-1) of alpha-quartz for 24 and 48 h produced a significant increase in LDH release. The concentration-time-dependent bleb induction evidenced by SEM correlates with the increase of apoptotic cells and LDH activity release, demonstrating the onset of cytotoxic effects in human lung cells exposed to alpha-quartz.

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“…4 In a recent multidisciplinary study [42][43][44][45] on industrial quartz dusts two were nearly inactive and two very active both in cellular toxicity and "in vivo" inflammogenicity. Inactivity was then mainly ascribed to their aluminium content, much higher in the two "less toxic" samples.…”

Section: Cytotoxicity Oxidative Stress and Inflammationmentioning

confidence: 99%

Carbon in Intimate Contact with Quartz Reduces the Biological Activity of Crystalline Silica Dusts

Ghiazza

Tomatis

Doublier

et al. 2012

Chem. Res. Toxicol.

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In order to evaluate the effect of carbonaceous materials on the pathogenic activity of quartz dusts, mixtures of carbon soot (1% and 10%) and quartz (Min-U-Sil ) have been prepared, then milled so to attain an intimate association of carbon and the quartz surface. Both cellular and cell-free tests show that carbon associated to quartz completely inhibits the typical free radical generation of quartz dusts (through Fenton activity and homolytic cleavage of a C-H bond) and suppresses the oxidative stress and inflammation induced by quartz alone on MH-S murine macrophage cells (lipid peroxidation, nitric oxide release, tumour necrosis factor-α synthesis). Cytotoxic response to quartz is also largely reduced. An extremely pure quartz milled with 10% of soot showed inactivating effects on the adverse reactions to quartz similar to Min-U-Sil quartz. None of these effects takes place when the same experiments are carried out with mechanically mixed samples, which suggests that carbon acts not just as a radical quencher, but because of its association to the quartz surface.4

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Different toxic, fibrogenic and mutagenic effects of four commercial quartz flours in the rat lung

Cited by 24 publications

References 21 publications

Toxicity of a Quartz with Occluded Surfaces in a 90-Day Intratracheal Instillation Study in Rats

Toxicity of a Quartz with Occluded Surfaces in a 90-Day Intratracheal Instillation Study in Rats

Evaluation of cytotoxic concentration–time response in A549 cells exposed to respirable α‐quartz

Carbon in Intimate Contact with Quartz Reduces the Biological Activity of Crystalline Silica Dusts

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