Different thermotolerances in <i>in vitro</i>‐produced embryos derived from different maternal and paternal genetic backgrounds

Sakatani, Miki; Yamanaka, Kenichi; Balboula, Ahmed Z.; Takahashi, Masashi

doi:10.1111/asj.12875

Cited by 5 publications

(5 citation statements)

References 27 publications

(51 reference statements)

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“…It has been reported that any source of stress, including HS, has the potential to disrupt the process of interactions between the uterus and the embryo (Thatcher, Meyer, & Danet-Desnoyers, 1995), consequently compromising the establishment and maintenance of pregnancy. Although some studies have shown heat-shocked bovine embryos have the competence and establish pregnancy (Khatun et al, 2018;Sakatani, Yamanaka, Balboula, & Takahashi, 2017), it is evident HS influences embryo development. Also, an in vitro model of bovine embryo production has been used to mimic HS on lactating dairy cows during summer days (Nabenishi et al, 2011(Nabenishi et al, , 2012.…”

mentioning

confidence: 98%

Heat stress on oocyte or zygote compromises embryo development, impairs interferon tau production and increases reactive oxygen species and oxidative stress in bovine embryos produced in vitro

Amaral

Koch

Júnior

et al. 2020

Molecular Reproduction Devel

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Interferon tau (IFNT) is the cytokine responsible for the maternal recognition of pregnancy in ruminants and plays a role modulating embryo–maternal communication in the oviduct inducing a local response from immune cells. We aimed to investigate IFNT production, reactive oxygen species, and oxidative stress under the influence of heat stress (HS) during different stages of bovine in vitro embryo production. HS was established when the temperature was gradually raised from 38.5°C to 40.5°C in laboratory incubator, sustained for 6 hr, and decreased back to 38.5°C. To address the HS effects on IFNT production, reactive oxygen species, and oxidative stress, ovaries from a slaughterhouse were used according to treatments: control group (38.5°C); oocytes matured under HS; oocytes fertilized under HS; zygotes cultured in the first day under HS; and cells submitted to HS at oocyte maturation, fertilization, and the first day of zygote culture. The HS negatively affected cleavage and blastocyst rates, in all HS groups. On Day 7, all HS‐treated embryos showed decrease IFNT gene and protein expressions, whereas reactive oxygen species were increased in comparison to the control. In conclusion, the compromised early embryo development due to higher temperatures during in vitro oocyte maturation, fertilization, and/or zygote stage have diminished IFNT expression and increased reactive oxygen species in bovine.

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mentioning

confidence: 98%

Heat stress on oocyte or zygote compromises embryo development, impairs interferon tau production and increases reactive oxygen species and oxidative stress in bovine embryos produced in vitro

Amaral

Koch

Júnior

et al. 2020

Molecular Reproduction Devel

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“…The lower lipid peroxide end product (i.e., MDA) and the higher GSH levels could probably be interpreted by a direct and/or an indirect antioxidant action of melatonin. Especially, glutathione, a scavenger of H 2 O 2 , is the major antioxidant component for scavenging ROS at oocyte level [7] and exerts a crucial role in embryonic developmental competence at preimplantation stages. When glutathione is added in IVM culture medium, the in vitro tolerance of bovine embryos to heat stress at 42 • C is increased [40].…”

Section: Discussionmentioning

confidence: 99%

“…Almost all the reproductive processes, from the stage of follicular growth and oocyte maturation to parturition, as well as lactation and uterus involution, could be affected by heat stress. However, in farm animals the principal adverse effect of heat stress on reproduction is the low fertility rate, mainly due to high susceptibility of preovulatory follicles and of preimplantation stage embryos to high maternal temperature [6,7]. The early developmental stages from oocytes to cleavage-stage are thermosensitive, while advanced developmental stages of early embryos, such as morulae and blastocysts are thermotolerant.…”

Section: Introductionmentioning

confidence: 99%

“…The early developmental stages from oocytes to cleavage-stage are thermosensitive, while advanced developmental stages of early embryos, such as morulae and blastocysts are thermotolerant. During these developmental stages, thermosensitivity is related to increased oxidative stress levels [7]. Accumulating evidence indicate that ROS and antioxidant systems participate in reproductive processes, such as follicular growth, ovulation, fertilization, luteal steroidogenesis, implantation and developmental competence of embryo(s) [8].…”

Section: Introductionmentioning

confidence: 99%

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Redox Status and Hematological Variables in Melatonin-Treated Ewes during Early Pregnancy under Heat Stress

Bouroutzika

Theodosiadou

Barbagianni

et al. 2022

Veterinary Sciences

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The preovulatory follicles and preimplantation stage embryos are found to be rather sensitive to heat stress due to their low potential for scavenging reactive oxygen species (ROS). The aim of the present study was to assess the impact of melatonin administration on redox status and hematological variables during the preovulatory period and early stages of embryogenesis in heat-stressed ewes in vivo. Forty Karagouniko-breed ewes were divided in two groups, the melatonin (M, n = 20) group and control (C, n = 20) one. All animals were subjected to heat stress throughout the study, which lasted forty days (D0 to D40). In M group, melatonin implants were administered on D0. Then, oestrous synchronization was applied (D19-D33). On D34, six rams were introduced into the ewe flock for mating. Ultrasonographic examination was conducted on D73 for pregnancy diagnosis. The temperature humidity index (THI), the rectal temperature (RT), and the number of breaths per minute (BR) were evaluated twice daily. Redox biomarkers, namely total antioxidant capacity (TAC), reduced glutathione (GSH), and thiobarbituric acid reactive substances (TBARS), were assayed in blood samples collected on D0, D33, and D40. In addition, packed cell volume (PCV), white blood cells (WBCs), leukocyte differential count, and cortisol assessment were conducted in blood samples on D33 and D40. The results indicated improved fertility rate and mean number of lambs born per ewe due to improved redox status (p < 0.05) in ewes that received melatonin implants 34 days approximately before the onset of oestrus. The PCV decreased in both groups between the two time-points (p < 0.05). However, the NEU/LYMPH ratio decreased (p < 0.05) only in group M. The low cortisol levels and the decreased NEU/LYMPH ratio in both groups support the hypothesis that ewes of the indigenous Karagouniko breed may exhibit adaptation to environmental thermal stress. The administration of melatonin as an antioxidant regime may improve the reproductive competence of heat stressed ewes and may also enhance their ability to adapt at high ambient temperatures.

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“…During summers, a negative energy balance is caused by a decrease in feed intake. As a result, steroid metabolism, which controls sex hormone secretion, is adversely affected, causing ovarian dysfunction [ 12 , 14 , 15 , 16 ]. Breeding of cattle is mostly carried out by artificial insemination (AI) in Japan.…”

Section: Decline In Livestock Fertility and Global Warmingmentioning

confidence: 99%

[The role of reproductive biology in SDGs] Global warming and cattle reproduction: Will increase in cattle numbers progress to global warming?

Sakatani

2022

Journal of Reproduction and Development

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The livestock industry produces a large amount of greenhouse gases (GHG) that cause global warming. A high percentage of GHG emissions are derived from cattle and has been suggested to be a factor in global warming. With the global increase in the consumption of livestock products, the number of farm animals has increased. In addition, the reduction in productivity and reproductive capacity of cattle has resulted in accelerated GHG emissions. In a high-temperature environment, the pregnancy rate decreases, leading to an increase in animals that do not contribute to production.Consequently, GHG emission per unit product increases, thereby accelerating global warming. To reduce this environmental impact, it is important to improve the breeding efficiency of cattle by the use of reproductive technology and, thus, reduce the number of non-productive animals. Thus, reproductive biology plays a major role in mitigating global warming related to the livestock industry.

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Different thermotolerances in in vitro‐produced embryos derived from different maternal and paternal genetic backgrounds

Cited by 5 publications

References 27 publications

Heat stress on oocyte or zygote compromises embryo development, impairs interferon tau production and increases reactive oxygen species and oxidative stress in bovine embryos produced in vitro

Heat stress on oocyte or zygote compromises embryo development, impairs interferon tau production and increases reactive oxygen species and oxidative stress in bovine embryos produced in vitro

Redox Status and Hematological Variables in Melatonin-Treated Ewes during Early Pregnancy under Heat Stress

[The role of reproductive biology in SDGs] Global warming and cattle reproduction: Will increase in cattle numbers progress to global warming?

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