Different Suppressive Effects of Combined Cyclophosphamide-Antigen Treatment Compared with Exclusive Cyclophosphamide Treatment on Primary and Secondary Humoral Immune Reactivity

Herrlinger, J. D.; Müller-Ruchholtz, W

doi:10.1159/000232087

Cited by 8 publications

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cytotoxic immunosuppression deletes lymphocytes, especially while they actively proliferate following immunization [30]. Cyclophosphamide is active throughout the cell cycle, but particularly during the S-phase when it deletes proliferating cells by cross-linking their DNA.…”

Section: Immunization Combined With Cytotoxic Immunosuppressionmentioning

confidence: 99%

Immunologic rebound

Dau

1995

J of Clinical Apheresis

View full text Add to dashboard Cite

Evidence for accelerated specific antibody (Ab) rebound or overshoot after single or multiple therapeutic plasmapheresis (TP) is fragmentary but suggested by both clinical and experimental evidence. In vitro studies showing increased peripheral blood lymphocyte turnover and total immunoglobulin production after a series of TP without immunosuppression may signify a generalized immunostimulation through removal of regulatory molecules by TP. It is known that IgG class Ab can down regulate B cells by cross linkage of their Ag and Fc receptors. Cyclophosphamide and other cytotoxic immunosuppressive agents effectively delete proliferating lymphocytes. TP could particularly foster deletion of lymphocytes actively mediating autoimmunity, since they would be more readily stimulated to proliferation by removal of Ab or other inhibitory factors than the generally resting normal immune system. This is supported by a relatively greater reduction of autoantibody levels than total immunoglobulin after treatment with TP and cytotoxic immunosuppressives.

show abstract

Section: Immunization Combined With Cytotoxic Immunosuppressionmentioning

confidence: 99%

Immunologic rebound

Dau

1995

J of Clinical Apheresis

View full text Add to dashboard Cite

show abstract

Treatment‐free remission in severe systemic lupus erythematosus following synchronization of plasmapheresis with subsequent pulse cyclophosphamide

Euler

Schroeder²,

Harten³

et al. 1994

Arthritis & Rheumatism

134

View full text Add to dashboard Cite

Objective. To investigate the effect of an intensified treatment protocol synchronizing plasmapheresis with subsequent pulse cyclophosphamide for severe systemic lupus erythematosus (SLE).Methods. A protocol of plasmapheresis (3 X 60 mllkg) and subsequent high-dose pulse cyclophosphamide (36 mgkg) followed by 6 months of peroral immunosuppression was used to treat 14 patients with severe SLE.Results. Rapid improvement was achieved in all patients. Immunosuppressants, including corticosteroids, were withdrawn at month 6 in 12 patients. Eight patients continued without treatment for a mean observation period of 5.6 years (46-91 months).Conclusion. The results demonstrate that treatment-free clinical remission can be achieved in some patients with severe SLE.

show abstract

Evidence That X-Irradiation and 4-Hydroperoxycyclophosphamide Affects Different Lymphocytes That Respond to Specific Antigen In Vitro

Clark

LaSota

Borch

1985

Journal of Leukocyte Biology

View full text Add to dashboard Cite

The present study examined the effect of pulse treatment with the in vitro active synthetic derivative of cyclophosphamide (CY), 4-hydroperoxycyclophosphamide (4-HPCY), and exposure to X-irradiation on the in vitro Concanavalin A (ConA), lipopolysaccharide (LPS), and antigen-specific blastogenic responses of in vivo-primed lymph node cells. Primed lymph node cells from CY-pretreated, aggregated (A) human IgG-complete Freund's adjuvant (AHGG-CFA)-immunized mice were untreated, exposed to various doses of irradiation, or pulse treated with different concentrations of 4-HPCY before being cultured in medium alone or in medium containing HGG, ConA, or LPS. The results show that HGG-responding and LPS-responding cells exhibited similar dose-inactivation profiles following exposure to irradiation or pulse treatment with 4-HPCY. More than 75% of reactivity was eliminated by exposure to 100 rads or pulse treatment with 20 microM 4-HPCY. In contrast to preculture pulse treatment with 4-HPCY, however, when primed lymph node cells were cultured in medium containing 4-HPCY (culture treatment) LPS-responding cells were shown to be more sensitive to inactivation than HGG-responding cells. The data further show that the effect of low-dose irradiation and of culture treatment with 4-HPCY on the HGG-specific response of primed lymph node cells was additive, suggesting that these agents inactivate different cell subtypes that contribute to the HGG-specific response in vitro.

show abstract

Different Suppressive Effects of Combined Cyclophosphamide-Antigen Treatment Compared with Exclusive Cyclophosphamide Treatment on Primary and Secondary Humoral Immune Reactivity

Cited by 8 publications

References 16 publications

Immunologic rebound

Immunologic rebound

Treatment‐free remission in severe systemic lupus erythematosus following synchronization of plasmapheresis with subsequent pulse cyclophosphamide

Evidence That X-Irradiation and 4-Hydroperoxycyclophosphamide Affects Different Lymphocytes That Respond to Specific Antigen In Vitro

Contact Info

Product

Resources

About