Different subcellular storage sites for decidual- and pituitary-derived prolactin: possible explanation for differences in regulation

Handwerger, Stuart; Wilson, Steven P.; Pm, Conn

doi:10.1016/0303-7207(84)90130-8

Cited by 21 publications

(2 citation statements)

References 16 publications

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“…Furthermore, most studies have not distin¬ guished between regulation of de novo synthesis and regulation of secretion of decidual prolactin. Unlike pituitary prolactin, decidual prolactin is not pack¬ aged into secretory granules (Handwerger et al 1984). Thus there may be a close link between the level of prolactin gene transcription and mRNA abundance controlling the rate of synthesis and the rate of prolactin secretion.…”

Section: Introductionmentioning

confidence: 98%

The relationship between decidualization and prolactin mRNA and production at different stages of human pregnancy

Wen¹,

Brooks²,

Glasier³

et al. 1995

Journal of Molecular Endocrinology

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Within the human utero-placental unit only decidualized stromal cells express mRNA for prolactin. However, it is not clear if the level of prolactin production is related to the number of decidualized cells or the capacity of individual decidual cells to synthesize prolactin, either or both of which parameters may change during pregnancy. In the present study, prolactin production at different stages of human pregnancy was examined using quantitative in situ hybridization to assess decidual prolactin mRNA abundance, immunocytochemistry to examine the prolactin content inside decidual cells and RIA to measure decidual prolactin output into amniotic fluid. Throughout pregnancy the proportion of stromal cells showing positive immunostaining and mRNA for prolactin increased. There was a parallel increase in decidual cell size which was correlated with an increase in prolactin gene expression and intensity of immunostaining for prolactin in individual decidual cells. These changes in decidual cells were consistent with the changes in the concentration of prolactin in amniotic fluid. These results suggest that there is a close link between the level of prolactin gene expression and production of prolactin by individual decidual cells, which in turn is directly related to the process of decidualization that continues throughout human pregnancy.

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Section: Introductionmentioning

confidence: 98%

The relationship between decidualization and prolactin mRNA and production at different stages of human pregnancy

Wen¹,

Brooks²,

Glasier³

et al. 1995

Journal of Molecular Endocrinology

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“…2 The placenta synthesises and releases hCG and hPL which have striking chemical and biological similarities to LH and to growth hormone and prolactin, respectively.3 Nevertheless, despite the striking similarities between these hormones, the regulation of the synthesis and secretion of the protein hormones from the decidua and placenta is different from that of the pituitary protein hormones. Pituitary prolactin, growth hormone and most protein hormones are stored in large secretory granules, but ultrastructural and biochemical studies indicate that decidual prolactin 4 and hPL5 are localised in the postmicrosomal supernatants of decidual and placental tissue homogenates.…”

mentioning

confidence: 99%

Paracrine and Autocrine Factors Involved in the Regulation of the Release of Human Decidual Prolactin and Human Placental Lactogen

Handwerger

Golander

Richards

et al. 1989

Placenta as a Model and a Source

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During pregnancy, the decidua and placenta synthesise and secrete several protein hormones that have identical or nearly identical chemical and biological properties to protein hormones synthesised and secreted by the pituitary gland and other tissues. For example, human decidual tissue synthesises and releases protein hormones that are identical to pituitary prolactin 1 and ovarian relaxin. 2 The placenta synthesises and releases hCG and hPL which have striking chemical and biological similarities to LH and to growth hormone and prolactin, respectively.3 Nevertheless, despite the striking similarities between these hormones, the regulation of the synthesis and secretion of the protein hormones from the decidua and placenta is different from that of the pituitary protein hormones. Pituitary prolactin, growth hormone and most protein hormones are stored in large secretory granules, but ultrastructural and biochemical studies indicate that decidual prolactin 4 and hPL5 are localised in the postmicrosomal supernatants of decidual and placental tissue homogenates.We report here studies from our laboratory on the regulation of the synthesis and release of decidual prolactin and placental prolactin and present new evidence indicating novel regulatory pathways involved in the release of these hormones. Particular attention will be given to the role of autocrine and paracrine factors in the regulation of decidual prolactin. DECIDUAL PROLACTINOur initial studies of the synthesis and release of decidual prolactin indicated that TRH, dopamine, and bromocriptine had no effect on the synthesis or release of decidual prolactin, even at concentrations 1000 times the half-maximal concentrations that affect the synthesis and release of pituitary prolactin. 6 In addition, oestrogen7 and vasoactive intestinal polypeptide (unpublished observation), both of which stimulate pituitary prolactin release, had no effect on the release of decidual prolactin.Several studies subsequently demonstrated that exposure of endometrial tissue from the follicular phase of the menstrual cycle to progesterone resulted in decidualisation of the endometrium and in a striking increase in both the synthesis and release of prolactin. 8 Several investigators also observed that progesterone stimulated prolactin release from decidualised

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Prolactin

Sarapura¹

1997

Diseases of the Pituitary

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Different subcellular storage sites for decidual- and pituitary-derived prolactin: possible explanation for differences in regulation

Cited by 21 publications

References 16 publications

The relationship between decidualization and prolactin mRNA and production at different stages of human pregnancy

The relationship between decidualization and prolactin mRNA and production at different stages of human pregnancy

Paracrine and Autocrine Factors Involved in the Regulation of the Release of Human Decidual Prolactin and Human Placental Lactogen

Prolactin

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