Different specificities of two aldehyde dehydrogenases from <i>Saccharomyces cerevisiae var. boulardii</i>

Datta, Suprama; Annapure, Uday S.; Timson, David J.

doi:10.1042/bsr20160529

Cited by 12 publications

(11 citation statements)

References 69 publications

(63 reference statements)

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“…The distinctively different headspace volatile blends originating from dried fruit and fruit powder with and without yeast are consistent with reports that fruit fermentation by yeasts results in the production of higher alcohols and esters ( Palanca et al 2013 , Golonka et al 2014 ) and the reduction of aldehydes to carboxylic acids ( Liu and Moon 2009 , Datta et al 2017 ). One higher alcohol, 2-phenylethyl alcohol, originated only from baits containing yeast.…”

Section: Discussionsupporting

confidence: 90%

“…It is possible that the dried fruit and fruit powder, unlike fresh fruit, do not contain the specific precursors necessary for ester formation. S. cerevisiae possesses several aldehyde dehydrogenases and reductases, which convert aldehydes to carboxylic acids such as acetic acid ( Liu and Moon 2009 , Datta et al 2017 ). This explains the smaller number of aldehydes and the larger number of carboxylic acids in headspace volatile blends of dried fruit mixtures containing yeast.…”

Section: Discussionmentioning

confidence: 99%

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Brewer’s Yeast, Saccharomyces cerevisiae, Enhances Attraction of Two Invasive Yellowjackets (Hymenoptera: Vespidae) to Dried Fruit and Fruit Powder

Babcock

Gries

Borden³

et al. 2017

Journal of Insect Science

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The German yellowjacket, Vespula germanica F., and common yellowjacket, Vespula vulgaris L. (Hymenoptera: Vespidae), are pests of significant economic, environmental, and medical importance in many countries. There is a need for the development and improvement of attractive baits that can be deployed in traps to capture and kill these wasps in areas where they are a problem. Yellowjackets are known to feed on fermenting fruit, but this resource is seldom considered as a bait due to its ephemeral nature and its potential attractiveness to nontarget species. We analyzed the headspace volatiles of dried fruit and fruit powder baits with and without Brewer’s yeast, Saccharomyces cerevisiae, using gas chromatography–mass spectrometry, and we field tested these baits for their attractiveness to yellowjackets in Argentina. The addition of yeast to dried fruit and fruit powder changed the volatile compositions, increasing the number of alcohols and acids and decreasing the number of aldehydes. Dried fruit and fruit powder baits on their own were hardly attractive to yellowjackets, but the addition of yeast improved their attractiveness by 9- to 50-fold and surpassed the attractiveness of a commercial heptyl butyrate-based wasp lure. We suggest that further research be done to test additional varieties and species of yeasts. A dried fruit or fruit powder bait in combination with yeast could become a useful tool in the management of yellowjackets.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Brewer’s Yeast, Saccharomyces cerevisiae, Enhances Attraction of Two Invasive Yellowjackets (Hymenoptera: Vespidae) to Dried Fruit and Fruit Powder

Babcock

Gries

Borden³

et al. 2017

Journal of Insect Science

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“…While the relative activities of 17 of the 18 promoters in S. boulardii were similar to reported values in S. cerevisiae , one promoter ( pALD6 ) showed 10-fold higher activity in S. boulardii than in S. cerevisiae (Supplementary Figure S4). While the precise role of ALD6 in the probiotic properties of S. boulardii is unknown, Ald6p is involved in the detoxification of aldehyde molecules to their corresponding acid in S. cerevisiae 45 . Collectively, this data curates a set of promoters enabling precise tuning of gene expression in S. boulardii , and indicates that constructs developed for S. cerevisiae will exhibit similar gene expression profiles in S. boulardii .…”

Section: Resultsmentioning

confidence: 99%

Establishing ProbioticSaccharomyces boulardiias a Model Organism for Synthesis and Delivery of Biomolecules

Durmusoglu

Al'Abri

Collins

et al. 2020

Preprint

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12Saccharomyces boulardii is a widely used yeast probiotic which can counteract various 13 gastrointestinal disorders 1 . As a relative of Saccharomyces cerevisiae, S. boulardii exhibits rapid 14 growth and is easy to transform 2 and thus represents a promising chassis for the engineered 15 secretion of biomolecules. To establish S. boulardii as a platform for delivery of biomolecules to 16 the mammalian gut, we measured the amount and variance in protein expression enabled by 17 promoters, terminators, selective markers, and copy number control elements in this organism. 18These genetic elements were characterized in plasmidic and genomic contexts, revealing 19 strategies for tunable control of gene expression and CRISPR-mediated genome editing in this 20 strain. We then leveraged this set of genetic parts to combinatorially assemble pathways 21 enabling a wide range of drug and vitamin titers. Finally, we measured S. boulardii's residence 22 time in the gastrointestinal tracts of germ-free and antibiotic-treated mice, revealing the 23 relationships between dosing strategy and colonization level. This work establishes S. boulardii 24 as a genetically tractable commensal fungus and provides a set of strategies for engineering S. 25 boulardii to synthesize and deliver biomolecules during gut colonization. 26 27 abundant in the human gut, 21 and difficulty in producing high levels of post-translationally-42 modified proteins 22 . 43 44In addition to bacteria, a diverse fungal population also exists in the human gastrointestinal tract 45 (GIT) [23][24][25][26] . While the numerical abundance of fungal cells in the GIT is much lower than bacterial 46 cells, fungal cells are on average 100-fold larger, indicating that their biomass and role in the gut 47 may be larger than metagenomic surveys indicate 25,27 . Recent studies have shown that while 48 3 commensal fungi play important roles in the development of inflammatory bowel diseases, they 49 also provide protective heterologous immunity to pathogenic organisms by training the immune 50 system 28,29 . Furthermore, fungi are not susceptible to bacteriophage predation and are easily 51 engineered to secrete high titers of proteins that are post-translationally modified. Thus, in this 52 study, we established a pipeline to engineer the only eukaryotic probiotic that is approved by the 53 FDA: Saccharomyces boulardii 30 . 54 55 S. boulardii is a non-pathogenic yeast that was originally isolated from lychee and mangosteen in 56 1923 and has since been used to treat ulcerative colitis, diarrhea, and recurrent Clostridium 57 difficile infection 1,31 . S. boulardii is closely related to the famous budding yeast Saccharomyces 58 cerevisiae, indicating that it may be similarly amenable to engineering for the production of 59 biomolecules, including biologics requiring post-translational modifications 2,32 . Supporting this, 60 S. cerevisiae expression vectors can be transformed and propagated in S. boulardii, and 61 CRISPR/Cas9-mediated genome editing is functional in both ...

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“…We examined the role of six gene(s) ( ALD2 - ALD6 , HFD1 ) encoding enzymes with aldehyde dehydrogenase activity 34,35 in 4MAB acid production. ALD2 and ALD3 encode nearly identical cytosolic dehydrogenases, which catalyze the oxidation of 3-aminopropanal to β-alanine 36 .…”

Section: Resultsmentioning

confidence: 99%

Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids

Srinivasan

Smolke

2019

Nat Commun

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Tropane alkaloids (TAs) are a class of phytochemicals produced by plants of the nightshade family used for treating diverse neurological disorders. Here, we demonstrate de novo production of tropine, a key intermediate in the biosynthetic pathway of medicinal TAs such as scopolamine, from simple carbon and nitrogen sources in yeast ( Saccharomyces cerevisiae ). Our engineered strain incorporates 15 additional genes, including 11 derived from diverse plants and bacteria, and 7 disruptions to yeast regulatory or biosynthetic proteins to produce tropine at titers of 6 mg/L. We also demonstrate the utility of our engineered yeast platform for the discovery of TA derivatives by combining biosynthetic modules from distant plant lineages to achieve de novo production of cinnamoyltropine, a non-canonical TA. Our engineered strain constitutes a starting point for future optimization efforts towards realizing industrial fermentation of medicinal TAs and a platform for the synthesis of TA derivatives with enhanced bioactivities.

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Different specificities of two aldehyde dehydrogenases from Saccharomyces cerevisiae var. boulardii

Cited by 12 publications

References 69 publications

Brewer’s Yeast, Saccharomyces cerevisiae, Enhances Attraction of Two Invasive Yellowjackets (Hymenoptera: Vespidae) to Dried Fruit and Fruit Powder

Brewer’s Yeast, Saccharomyces cerevisiae, Enhances Attraction of Two Invasive Yellowjackets (Hymenoptera: Vespidae) to Dried Fruit and Fruit Powder

Establishing ProbioticSaccharomyces boulardiias a Model Organism for Synthesis and Delivery of Biomolecules

Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids

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