Different sensitivities of the Na+/K+-ATPase isoforms to oxidants

Role of Intracellular Na + Kinetics in Preconditioned Rat Heart

“…34,35 There is the possibility that the mito K ATP channel interacts with Na ϩ /K ϩ -ATPase because the interac-tion between actin microfilament and the sarcolemmal K ATP channel 36 or mito K ATP channel has been demonstrated. 37 Because free radicals can alter the activity of Na 38 the alterations activated by the mito K ATP channel 39 may enhance the enzyme activity leading to [Na ϩ ] i kinetics in IPC hearts.…”

Direct influence of the sodium pump on the membrane potential of vomeronasal chemoreceptor neurones in frog.

“…All recordings reported here were obtained at room temperature from freshly. isolated cells with a long dendrite ,um), a piece of axon and numerous microvilli at the apex of the dendrite (D0ving, Trotier, Rosin & Holley, 1993;Trotier et al 1993Trotier et al , 1994 (Shattock & Matsuura, 1993;Huang, Wang, Askari, Zolotarjova & Ganjeizadeh, 1994 Sodium pump in vomeronasal neurones -138 mV (Vm = -88 + 20 mV, n = 56). Two-thirds of the cells showed membrane potentials more negative than -85 mV, most of them between -105 and -85 mV.…”

Role of phospholemman and the 70 kDa inhibitor protein in regulating Na+/K+ ATPase activity in pulmonary artery smooth muscle cells under U46619 stimulation

“…Huang et al [18] showed that the a 2 and a 3 isoforms are more susceptible than the a 1 isoform toward oxidants. In BPASMCs, oxidant induce the inhibitory activity of the 70 kDa inhibitor protein, which have different affinities toward different isoforms of NKA (a 2 isoform is more susceptible than a 1 ) resulting in the differential regulation of the NKA isoforms.…”

“…Recently, we have reported the presence of PLM in smooth muscle cells [11] and hence we investigated its involvement in this scenario. Huang et al [18] showed that different isoforms of NKA are differentially inhibited by oxidants. Since…”