Different ROS-Scavenging Properties of Flavonoids Determine Their Abilities to Extend Shelf Life of Tomato

Zhang, Yang; Stefano, Rosalba De; Robine, Marie; Butelli, Eugenio; Bulling, Katharina; Hill, Lionel; Rejzek, Martin; Martin, Cathie; Schoonbeek, Henk‐jan

doi:10.1104/pp.15.00346

Cited by 53 publications

(58 citation statements)

References 58 publications

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“…Various constitutive and induced plant phenolic compounds are thought to contribute to defense against microbial pathogens (Osbourn, 1996;Lattanzio et al, 2006), but not all phenolics have this effect (Henriquez et al, 2012;Zhang et al, 2015). In order to determine if flavan-3-ols have this function in planta, we screened five poplar genotypes for resistance against rust infection and quantified their phenolic contents.…”

Section: High Levels Of Catechin and Pas Are Associated With Resistanmentioning

confidence: 99%

Flavan-3-ols Are an Effective Chemical Defense against Rust Infection

et al. 2017

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Phenolic secondary metabolites are often thought to protect plants against attack by microbes, but their role in defense against pathogen infection in woody plants has not been investigated comprehensively. We studied the biosynthesis, occurrence, and antifungal activity of flavan-3-ols in black poplar (Populus nigra), which include both monomers, such as catechin, and oligomers, known as proanthocyanidins (PAs). We identified and biochemically characterized three leucoanthocyanidin reductases and two anthocyanidin reductases from P. nigra involved in catalyzing the last steps of flavan-3-ol biosynthesis, leading to the formation of catechin [2,3-trans-(+)-flavan-3-ol] and epicatechin [2,3-cis-(2)-flavan-3-ol], respectively. Poplar trees that were inoculated with the biotrophic rust fungus (Melampsora larici-populina) accumulated higher amounts of catechin and PAs than uninfected trees. The de novo-synthesized catechin and PAs in the rust-infected poplar leaves accumulated significantly at the site of fungal infection in the lower epidermis. In planta concentrations of these compounds strongly inhibited rust spore germination and reduced hyphal growth. Poplar genotypes with constitutively higher levels of catechin and PAs as well as hybrid aspen (Populus tremula 3 Populus alba) overexpressing the MYB134 transcription factor were more resistant to rust infection. Silencing PnMYB134, on the other hand, decreased flavan-3-ol biosynthesis and increased susceptibility to rust infection. Taken together, our data indicate that catechin and PAs are effective antifungal defenses in poplar against foliar rust infection.

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Section: High Levels Of Catechin and Pas Are Associated With Resistanmentioning

confidence: 99%

Flavan-3-ols Are an Effective Chemical Defense against Rust Infection

et al. 2017

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“…One significant feature of flavonoids is their high ROS-scavenging capacity, which is attributed to their ability to protect cells from oxidative damage under UV light, fungal infection, and insect herbivory (Clayton et al, 2018;Heim, Tagliaferro, & Bobilya, 2002;Stracke, Favory, et al, 2010;Zhang et al, 2013;Zhang et al, 2015). Defective flavonoid accumulation resulted in enhanced H 2 O 2 in plant tissues (Nakabayashi et al, 2014).…”

Section: Glycosidic Flavonoids Mitigate Ros Damage Under Osmotic Stmentioning

confidence: 99%

“…Defective flavonoid accumulation resulted in enhanced H 2 O 2 in plant tissues (Nakabayashi et al, 2014). Although flavonoids have been implicated in diverse biological processes (Gou, Felippes, Liu, Weigel, & Wang, 2011;Heim et al, 2002;Yang et al, 2016;Zhang et al, 2013;Zhang et al, 2015), the direct link between an individual isoform and its physiological effect on osmotic tolerance remains to be determined. This study shows that the SQD2.1-derived glycosidic flavonoids, in particular A7G, mitigate ROS accumulation and reduce oxidative damage under osmotic stress (Figures 8 and 9).…”

Section: Glycosidic Flavonoids Mitigate Ros Damage Under Osmotic Stmentioning

confidence: 99%

“…Glycosidic flavonoids are predominant forms present in plant tissues (Sawada et al, 2005;Zhang et al, 2015;Zhao & Dixon, 2010), where glycosylation facilitates flavonoid stability, solubility, mobility, and biological activity with reduced toxicity in cells (Agati et al, 2012;Bowles et al, 2005;Stracke, Jahns, et al, 2010). Glycosylated flavonoids with increased hydrophilicity in cells may enhance their access to ROS molecules, mitigating ROS damage to cellular components such as proteins, DNA, and membrane lipids (Heim et al, 2002;Stracke, Favory, et al, 2010;Yang et al, 2016).…”

Section: Glycosidic Flavonoids Mitigate Ros Damage Under Osmotic Stmentioning

confidence: 99%

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Rice sulfoquinovosyltransferase SQD2.1 mediates flavonoid glycosylation and enhances tolerance to osmotic stress

Zhan

Shen

Chen

et al. 2019

Plant Cell & Environment

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Sulfoquinovosyltransferase 2 (SQD2) catalyses the final step in the sulfoquinovosyldiacylglycerol (SQDG) biosynthetic pathway. It is involved in the phosphate starvation response. Here, we show that rice SQD2.1 has dual activities catalysing SQDG synthesis and flavonoid glycosylation. SQD2.1 null mutants (sqd2.1) in rice had decreased levels of glycosidic flavonoids, particularly apigenin 7‐O‐glucoside (A7G), whereas these metabolites were increased in rice plants overexpressing SQD2.1. The sqd2.1 mutants and SQD2.1 overexpressing lines showed reduced and enhanced, respectively, tolerance to salinity and drought. Treating the sqd2.1 mutants with A7G decreased oxidative damage and restored stress tolerance to the wild‐type levels. These findings demonstrate that SQD2.1 has a novel function in the glycosylation of flavonoids that is required for osmotic stress tolerance in rice. The novel activity of SQD2.1 in the production of glycosidic flavonoids improves scavenging of reactive oxygen species and protects against excessive oxidation.

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“…In a recent study involving 24 apple varieties, correlation analysis revealed that phenols like flavonols and procyanidins B2 in the peel fraction of the inoculated fruit have a significant impact on blue mold resistance (Ahmadi-Afzadi et al, 2015). The comparison of tomato fruits that accumulate different levels of flavonoids and show different ripening behavior has also shed light on the role that specific flavonoids with high superoxide scavenging activities play in the higher resistance of some tomato lines to B. cinerea infection (Zhang et al, 2015).…”

Section: New Technologies For Studying Host-pathogen Interactions In mentioning

confidence: 99%

Molecular aspects in pathogen-fruit interactions: Virulence and resistance

Tian

Torres

Ballester

et al. 2016

Postharvest Biology and Technology

142

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Fruit losses during postharvest storage and handling due to pathogen infections are one of the major problems in the global food chain supply. The application of chemical fungicides to control diseases is currently limited by legislation in some countries and also raises concerns about food and environmental safety. Exploring molecular aspects of pathogen-fruit interactions therefore has biological and economic significance as a means to help develop rational alternatives for disease control. In this review we present the current knowledge of molecular aspects in pathogen-fruit interactions, addressing the following topics: the application of new "omics" technologies for studying these interactions; the molecular mechanisms of fungal pathogen attack; the regulation of virulence by exogenous factors; and, finally, fruit defense mechanisms.

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Different ROS-Scavenging Properties of Flavonoids Determine Their Abilities to Extend Shelf Life of Tomato

Cited by 53 publications

References 58 publications

Flavan-3-ols Are an Effective Chemical Defense against Rust Infection

Flavan-3-ols Are an Effective Chemical Defense against Rust Infection

Rice sulfoquinovosyltransferase SQD2.1 mediates flavonoid glycosylation and enhances tolerance to osmotic stress

Molecular aspects in pathogen-fruit interactions: Virulence and resistance

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