Different roles of glutathione in copper and zinc chelation in Brassica napus roots

Zlobin, Ilya E.; Карташов, А. В.; Shpakovski, George V.

doi:10.1016/j.plaphy.2017.06.029

Cited by 42 publications

(17 citation statements)

References 44 publications

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“…Even though several studies have indicated that a rapid and transient depletion of GSH occurs after exposure to excess metal concentrations, little is known about the impact of this event on the plant’s responses [ 10 , 53 , 54 ]. Indeed, depletion of this prominent anti-oxidant could lead to an oxidative challenge at the root level as its anti-oxidative capacity is largely impaired.…”

Section: Discussionmentioning

confidence: 99%

Identifying the Pressure Points of Acute Cadmium Stress Prior to Acclimation in Arabidopsis thaliana

Deckers

Hendrix

Prinsen

et al. 2020

IJMS

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The toxic metal cadmium (Cd) is a major soil pollutant. Knowledge on the acute Cd-induced stress response is required to better understand the triggers and sequence of events that precede plant acclimation. Therefore, we aimed to identify the pressure points of Cd stress using a short-term exposure set-up ranging from 0 h to 24 h. Acute responses related to glutathione (GSH), hydrogen peroxide (H2O2), 1-aminocyclopropane-1-carboxylic acid (ACC), ethylene and the oxidative challenge were studied at metabolite and/or transcript level in roots and leaves of Arabidopsis thaliana either exposed or not to 5 µM Cd. Cadmium rapidly induced root GSH depletion, which might serve as an alert response and modulator of H2O2 signalling. Concomitantly, a stimulation of root ACC levels was observed. Leaf responses were delayed and did not involve GSH depletion. After 24 h, a defined oxidative challenge became apparent, which was most pronounced in the leaves and concerted with a strong induction of leaf ACC synthesis. We suggest that root GSH depletion is required for a proper alert response rather than being a merely adverse effect. Furthermore, we propose that roots serve as command centre via a.o. root-derived ACC/ethylene to engage the leaves in a proper stress response.

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Section: Discussionmentioning

confidence: 99%

Identifying the Pressure Points of Acute Cadmium Stress Prior to Acclimation in Arabidopsis thaliana

Deckers

Hendrix

Prinsen

et al. 2020

IJMS

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“…Several compounds function as chelators to detoxify HMs in plant cells, including thiols such as reduced glutathione (GSH), phytochelatins (PCs), and metallothioneins (MTs) and nonthiol ligands such as histidine (His), nicotianamine (NA), oxalate, and organic acids (Alvarez-Fernández, Díaz-Benito, Abadía, -Millán, & Abadía, 2014). GSH can act as a low-affinity ligand for HMs in plants (Seth et al, 2012;Zlobin, Kartashov, & Shpakovski, 2017). HM-induced higher GSH levels are often observed in various plants (Drzewiecka et al, 2017;He et al, 2015;Shi et al, 2015).…”

Section: Hm Detoxification By Chelatorsmentioning

confidence: 99%

Physiological and molecular mechanisms of heavy metal accumulation in nonmycorrhizal versus mycorrhizal plants

Shi

Zhang

Chen

et al. 2018

Plant Cell & Environment

134

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Uptake, translocation, detoxification, and sequestration of heavy metals (HMs) are key processes in plants to deal with excess amounts of HM. Under natural conditions, plant roots often establish ecto‐ and/or arbuscular‐mycorrhizae with their fungal partners, thereby altering HM accumulation in host plants. This review considers the progress in understanding the physiological and molecular mechanisms involved in HM accumulation in nonmycorrhizal versus mycorrhizal plants. In nonmycorrhizal plants, HM ions in the cells can be detoxified with the aid of several chelators. Furthermore, HMs can be sequestered in cell walls, vacuoles, and the Golgi apparatus of plants. The uptake and translocation of HMs are mediated by members of ZIPs, NRAMPs, and HMAs, and HM detoxification and sequestration are mainly modulated by members of ABCs and MTPs in nonmycorrhizal plants. Mycorrhizal‐induced changes in HM accumulation in plants are mainly due to HM sequestration by fungal partners and improvements in the nutritional and antioxidative status of host plants. Furthermore, mycorrhizal fungi can trigger the differential expression of genes involved in HM accumulation in both partners. Understanding the molecular mechanisms that underlie HM accumulation in mycorrhizal plants is crucial for the utilization of fungi and their host plants to remediate HM‐contaminated soils.

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“…The unique nature of the thiol group helps to stabilize mercaptide–metal bonds. This type of stable bond formation in conjunction with higher water solubility presents GSH as a better scavenger against the toxicity of multiple metals (Gill et al, 2016a ; Zlobin et al, 2017 ), reactive oxygen species (ROS) (Gill et al, 2014 , 2015 , 2016b ), and other hazardous chemicals (Barrameda-Medina et al, 2017 ; Li et al, 2017 ). Previous reports have indicated that GSH plays a vital role in regulating the levels of oxygen singlet species (such as H 2 O 2 ) that can be induced by heavy metal stress in plant cells (Shao et al, 2008 ; Gill et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, several studies have reported the role of exogenous GSH to enhance the plant growth and development and the antioxidant defense in various plants under different abiotic stress i.e. high temperature (Nahar et al, 2015a ; Zhu et al, 2016 ), drought in mung bean (Nahar et al, 2015b ), isoproturon in wheat (Alla and Hassan, 2014 ), Cd in rice and barley (Chen et al, 2010 ; Cai et al, 2011 ), Pb +2 in Salvinia minima (Estrella-Gómez et al, 2012 ), Cr in rice (Qiu et al, 2013 ), salt in tomato plants (Zhou et al, 2016 , 2017 ), Cu and Zn in B. napus roots (Zlobin et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Reduced Glutathione Mediates Pheno-Ultrastructure, Kinome and Transportome in Chromium-Induced Brassica napus L.

et al. 2017

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Chromium (Cr) as a toxic metal is widely used for commercial purposes and its residues have become a potential environmental threat to both human and plant health. Oilseed rape (Brassica napus L.) is one of the candidate plants that can absorb the considerable quantity of toxic metals from the soil. Here, we used two cultivars of B. napus cvs. ZS 758 (metal-tolerant) and Zheda 622 (metal-susceptible) to investigate the phenological attributes, cell ultrastructure, protein kinases (PKs) and molecular transporters (MTs) under the combined treatments of Cr stress and reduced glutathione (GSH). Seeds of these cultivars were grown in vitro at different treatments i.e., 0, 400 μM Cr, and 400 μM Cr + 1 mM GSH in control growth chamber for 6 days. Results had confirmed that Cr significantly reduced the plant length, stem and root, and fresh biomass such as leaf, stem and root. Cr noticeably caused the damages in leaf mesophyll cells. Exogenous application of GSH significantly recovered both phenological and cell structural damages in two cultivars under Cr stress. For the PKs, transcriptomic data advocated that Cr stress alone significantly increased the gene expressions of BnaA08g16610D, BnaCnng19320D, and BnaA08g00390D over that seen in controls (Ck). These genes encoded both nucleic acid and transition metal ion binding proteins, and protein kinase activity (PKA) and phosphotransferase activities in both cultivars. Similarly, the presence of Cr revealed elite MT genes [BnaA04g26560D, BnaA02g28130D, and BnaA02g01980D (novel)] that were responsible for water transmembrane transporter activity. However, GSH in combination with Cr stress significantly up-regulated the genes for PKs [such as BnaCnng69940D (novel) and BnaC08g49360D] that were related to PKA, signal transduction, and oxidoreductase activities. For MTs, BnaC01g29930D and BnaA07g14320D were responsible for secondary active transmembrane transporter and protein transporter activities that were expressed more in GSH treatment than either Ck or Cr-treated cells. In general, it can be concluded that cultivar ZS 758 is more tolerant toward Cr-induced stress than Zheda 622.

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Different roles of glutathione in copper and zinc chelation in Brassica napus roots

Cited by 42 publications

References 44 publications

Identifying the Pressure Points of Acute Cadmium Stress Prior to Acclimation in Arabidopsis thaliana

Identifying the Pressure Points of Acute Cadmium Stress Prior to Acclimation in Arabidopsis thaliana

Physiological and molecular mechanisms of heavy metal accumulation in nonmycorrhizal versus mycorrhizal plants

Reduced Glutathione Mediates Pheno-Ultrastructure, Kinome and Transportome in Chromium-Induced Brassica napus L.

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