Different responses of heterogeneous graft presentations in bone reconstructions during sinus lift elevation surgery: an immunolabeling and histomorphometric study performed in rabbits

Abstract

Background: This study aims to analyze the process of bone formation, maturation and mineralization promoted by Cerabone ® functionalization with raloxifene in rabbit sinus floor elevation, through immunohistochemical and histomorphometric analyses.Methods: Twenty-four male rabbits had their maxillary sinuses filled. To access the sinus cavity a circular window with a diameter of 5 mm was made bilaterally to the midline of the nasal dorsum, the sinus membrane was detached and elevated using special curettes. Autogenous bone (G1), Cerabone ® (G2), Cerabone ® submitted to ultrasonic processing (G3), Cerabone ® associated to raloxifene submitted to ultrasonic processing (G4) were used to fill the maxillary sinus, 14 and 42 days postoperatively.Immunohistochemical and histomorphometric analyses were performed at 14 and 42 days postoperatively.Results: At 14 days postoperatively: the immunolabeling for vascular endothelial growth factor (VEGF) was moderate in groups G1, G3 and G4, while in group G2, the same protein was expressed in a mild way. The immunolabeling for alkaline phosphatase (ALP) was moderate in all experimental groups. Histomorphometric analysis showed a greater amount of newly formed bone in groups G1 (55.2%) and G4 (50.1%), which differed statistically from groups G2 (37.5%) and G3 (38.2%). At 42 days postoperatively: receptor activator of nuclear factor kappa-B ligand (RANKL) immunolabeling was slight in G1, intense in G2 and moderate in G3 and G4. The tartrate resistant acid phosphatase (TRAP) protein was slightly expressed in all experimental groups. The osteocalcin (OCN) protein was moderately expressed in the G1 group and intensely in the G2, G3 and G4 groups. As for the histomorphometric analysis, the greatest amount of neoformed bone was found in group G1 (83.5%), followed by group G4 (75.0%). Less neoformed bone was observed in G3 (56.7%), followed by G2 (64.2%). Statistical differences were observed between all experimental groups.

The Interplay of Raloxifene and Sonochemical Bio-Oss in Early Maxillary Sinus Bone Regeneration: A Histological and Immunohistochemical Analysis in Rabbits

“…In contrast, building on previous research [ 35 ], it is interesting to observe that the effectiveness of Raloxifene in promoting bone growth might vary depending on the context. A prior study by our team, using a different xenograft, found a 20% improvement in bone formation with Raloxifene, especially over an extended 42-day healing period.…”

Performance of Polydioxanone-Based Membrane in Association with 3D-Printed Bioceramic Scaffolds in Bone Regeneration

“…During image acquisition, the calibrated analyzer performed the histological description in each of the groups following previous studies of the research group. The images (4× magnification) were exported to Adobe Photoshop (Adobe Systems, version CC 2017, San Jose, CA, USA) and were merged to make a panoramic image and perform a descriptive analysis [ 41 ].…”