Different response of acetylcholinesterases in salt- and detergent-soluble fractions of honeybee haemolymph, head and thorax after exposure to diazinon

Glavan, Gordana; Kos, Monika; Božič, Janko; Drobne, Damjana; Sabotič, Jerica; Kokalj, Anita Jemec

doi:10.1016/j.cbpc.2017.12.004

Cited by 12 publications

(13 citation statements)

References 43 publications

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“…Considering that AmAChE1 expressed in the abdomen is mostly derived from the fat body 17 , expressed AmAChE1 is likely secreted from the fat body and circulates via hemolymph. Detection of soluble AChE with weak enzyme activity in honey bee hemolymph supports this notion 28 . Thus, the heat-induced expression of AmAChE1 appears to exert systemic effects on the whole body.…”

Section: Discussionmentioning

confidence: 83%

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Differential expression of acetylcholinesterase 1 in response to various stress factors in honey bee workers

Kim¹,

Kim²,

Lee³

et al. 2019

Sci Rep

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The honey bee acetylcholinesterase 1 (AmAChE1) has been suggested to be related to stress response as judged from its elevated expression level under brood rearing-suppressed conditions. To further investigate the involvement of AmAChE1 expression in the stress response and its physiological functions, we analyzed altered expression profiles of AmAChE1 induced by diverse stress factors. In addition, transcription profiles of several heat shock protein (Hsp) genes ( hsps ) and the vitellogenin (Vg) gene ( vg ) known as general stress markers were investigated as positive references. Among the tested stress conditions, AmAChE1 expression was induced under the brood rearing-suppressed, crowding and heat shock conditions. The hsps , particularly hsp70 and hsp90 , responded to seven of nine stress conditions tested, confirming that hsp expression profiles can serve as a general stress marker. Taken together, AmAChE1 expression is not suitable for using as a stress marker due to its limited response. Nevertheless, AmAChE1 expression appears to be connected, at least in part, to heat shock response and other pathways. Considering that AmAChE1 likely regulates the ACh titer particularly in non-neuronal tissues, thereby modulating the signal cascades mediated by mAChR, the AmAChE1 expression profile under different conditions likely provides important information on its physiological roles in honey bees.

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Section: Discussionmentioning

confidence: 83%

“…AmAChE1 is soluble like the mammalian AChE-R and distributed in both neuronal and non-neuronal tissues, including fat body and hemolymph 17,27,28 . The abundant expression of AmAChE1 in diverse non-neuronal tissues suggests its non-neuronal functions.…”

Section: Discussionmentioning

confidence: 99%

Differential expression of acetylcholinesterase 1 in response to various stress factors in honey bee workers

Kim¹,

Kim²,

Lee³

et al. 2019

Sci Rep

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“…They were not treated with any chemical substances at least four weeks prior to the experiments. Chronic feeding exposure to diazinon (5 mg L −1 ; Diazinon PESTANAL® standard, Sigma-Aldrich Co) was performed as reported by Glavan et al (2018). Briefly, the honeybees were allocated to four test cages with 15-20 bees per cage and transferred to an incubator in darkness (34 °C, 60% RH; Kambič d.o.o.,Slovenia)).…”

Section: Methodsmentioning

confidence: 99%

Histochemical Staining of Acetylcholinesterase in Carnolian Honeybee (Apis mellifera carnica) Brain after Chronic Exposure to Organophosphate Diazinon

Glavan

2020

Journal of Apicultural Science

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AbstractOrganophosphate insecticides are known to inhibit the activity of enzyme acetylcholinesterase. They affect olfactory learning and memory formation in honeybees. These insecticides cause mushroom body inactivation in honeybees, but their influence on other brain regions involved in olfactory perception and memory is unknown. The goal of this study was to study the effects of organophosphate insecticide diazinon on carnolian honeybee (Apis mellifera carnica) acetylcholinesterase activity in the olfactory brain regions of antennal lobe, mushroom body and lateral procerebrum (lateral horn). The lamina, medulla and lobula of optic lobes were also analyzed. The level of acetylcholinesterase activity was visualized using the histochemical staining method. Densitometric analysis of histochemical signals indicated that diazinon inhibited acetylcholinesterase activity only in the lip of calyces of mushroom body, but not in other analyzed olfactory regions, antennal lobe and lateral procerebrum. The visual brain system optic lobes were also unaffected. This is in accordance with the literature reporting that mushroom body is the main brain center for olfactory learning and memory formation in honeybees.

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“…Активность ацетилхолинэстеразы определяли по методу Эллмана [Glavan et al, 2018], с небольшими изменениями. Инкубационная среда содержала 50 мкл гомогената, 50 мкл 50 мМ фосфатного буфера рН = 7,0 и 100 мкл реагента Эллмана (50 мкл 2 мМ ацетилтиохолин йодида и 50 мкл 0,23 мМ DTNB смешивали непосредственно перед измерением).…”

Section: материалы и методыunclassified

“…Оптическую плотность измеряли при 405 нм в режиме кинетика в течение 30 мин при температуре 30°C. Активность АХЭ выражали в мкмоль гидролизованного ацетилтиохолин йодида/мин/мг белка, используя коэффициент молярной экстинкции 13,600 M -1 cm -1 [Glavan et al, 2018].…”

Section: материалы и методыunclassified

Phosphatase and acetylcholinesterase activities in different stages of the life cycle of the housefly Musca domestica L.

et al. 2020

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Всероссийский научно-исследовательский институт ветеринарной энтомологии и арахнологии -филиал Федерального государственного бюджетного учреждения науки Федерального исследовательского центра Тюменского научного центра Сибирского отделения Российской академии наук, ул. Институтская 2, Тюмень 625041 Россия.

show abstract

Different response of acetylcholinesterases in salt- and detergent-soluble fractions of honeybee haemolymph, head and thorax after exposure to diazinon

Cited by 12 publications

References 43 publications

Differential expression of acetylcholinesterase 1 in response to various stress factors in honey bee workers

Differential expression of acetylcholinesterase 1 in response to various stress factors in honey bee workers

Histochemical Staining of Acetylcholinesterase in Carnolian Honeybee (Apis mellifera carnica) Brain after Chronic Exposure to Organophosphate Diazinon

Phosphatase and acetylcholinesterase activities in different stages of the life cycle of the housefly Musca domestica L.

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