Different regeneration potential of various sunflower (Helianthus annuus L.) genotypes in meristem culture

Šamaj, Jozef; Auxtová, O.; Bobák, M.

doi:10.1007/bf02921106

Cited by 2 publications

(1 citation statement)

References 6 publications

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“…Several methods for sunflower regeneration have been reported (reviewed by Alibert et al, 1994), including organogenesis (Knittel et al, 1991;Pugliesi et al, 1991;Ceriani et al, 1992;Chraibi et al, 1992); somatic embryogenesis (Pelissier et al, 1990;Jeannin and Hahne, 1991;Fiore et al, 1997;Laparra et al, 1997); and shoot proliferation from meristematic tissue. Most recent studies on sunflower regeneration by direct organogenesis report a dependency on both culture conditions (Chraibi et al, 1992) and genotype (Samaj et al, 1994;Sarrafi et al, 1996;Fiore et al, 1997). However, sunflower regeneration to date is characterized by low efficiency, reliability, and reproducibility (Alibert et al, 1994).…”

Section: Introductionmentioning

confidence: 97%

A shoot regeneration protocol effective on diverse genotypes of sunflower (Helianthus annuus L.)

Shin

Kim

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

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We describe a protocol, and several experiments that helped lead to its development, for sunflower regeneration. Important factors for sunflower regeneration were explant age, cytokinin type and concentration, basal medium, and explant source. We could not induce shoot regeneration from the explants derived from mature tissues including leaf, petiole, and stem. However, use of juvenile explants such as embryo meristem and primordial leaf tissues allowed routine regeneration of 17 different sunflower genotypes. High frequency of shoot regeneration was achieved with these explants taken from seedlings up to 5 d after germination. Explant age was less critical for embryo meristem explants than for primordial leaf tissues. Of the four basal media tested, MS and B5 media produced higher shoot-regeneration frequencies than did Anderson and woody plant media. The highest shoot-regeneration frequency was obtained with MS medium supplemented with 2 mM BA and without auxin. Addition of 1 mM naphthalene-acetic acid to the medium significantly reduced both the percentage of explants producing shoots and average number of shoots per explant. Regenerated shoots were grown to maturity in a greenhouse.

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Section: Introductionmentioning

confidence: 97%

A shoot regeneration protocol effective on diverse genotypes of sunflower (Helianthus annuus L.)

Shin

Kim

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

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Increase of callus and embryoid production from hypocotyl protoplasts of sunflower (Helianthus annuus L.) by culture in microdrops

Šamaj¹,

Okolot²,

Bobák³

et al. 1994

Biol. plant.

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Hypocotyls of seedling plants of the sunflower inbred line HNK-81 were used as a source of protoplasts. Optimal conditions of isolation and culture of protoplasts were established. Using the method of individual culture in microdrops, a 77 % final plating efficiency was achieved. Cytokinins, especially zeatin, showed a significant influence on the formation of globular embryo-like structures, but these failed to develop into mature embryos. Calli obtained in liquid media containing auxins and cytokinins grew on agar solidified media without growth regulators.

show abstract

Different regeneration potential of various sunflower (Helianthus annuus L.) genotypes in meristem culture

Cited by 2 publications

References 6 publications

A shoot regeneration protocol effective on diverse genotypes of sunflower (Helianthus annuus L.)

A shoot regeneration protocol effective on diverse genotypes of sunflower (Helianthus annuus L.)

Increase of callus and embryoid production from hypocotyl protoplasts of sunflower (Helianthus annuus L.) by culture in microdrops

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