The microstructure evolution during the drawing process of Ti-6Al-4V alloy with a lamellar morphology as an initial microstructure was investigated. Microstructure analysis on specimens with a different reduction ratio supported by 2D-drawing process simulation using DEFORMTM was utilized to examine the deformed state and microstructure behavior of the alloy. Dynamic recrystallization (DRX) phenomenon on the high reduction ratio (52.7%) was achieved fine equiaxed grain. A Zenner-Holllomon calculation using temperature and strain rates was also conducted to evaluate the DRX. Furthermore, a higher drawing reduction ratio attributed to a high fraction of kinked lamellar, which DRX occurred in the shear band and the regions of broken lath or kinked.