Different proportions of laccase isoenzymes produced by submerged cultures of Trametes versicolor grown on lignocellulosic wastes

Moldes, Diego; Lorenzo, María del Carmen Ricoy; Sanromán, M. Ángeles

doi:10.1023/b:bile.0000015452.40213.bf

Cited by 81 publications

(65 citation statements)

References 10 publications

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“…Then, it peaked on the day 8 (2247 U l −1 ) and hereafter it decreased. According to reports of Moldes et al (2004) barley bran cultures gave the highest lacasse activities, showing values of 2500-3200 U l −1 from the day 20 to the day 35, that of barley straw cultures exhibited values of about 300-500 U l −1 at the same days. Schlosser et al (1997) proposed the mechanism for these materials to induce the lacasse production is the presence of little quantities of phenolic soluble compounds.…”

Section: In This Study 20 Basidiomycetes Isolates Such As P Arcularmentioning

confidence: 83%

Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol

Gedikli¹,

Aytar²,

Ünal³

et al. 2010

Electron Journ of Biotechnol

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Lacasse is one of the extracellular enzymes excreted from white and brown rot fungi, which is involved in ligninolysis. In the present study, the effects of the addition of lacasse inducers to the medium on enhancement of enzyme production under conditions of submerged fermentation were researched. At first, a culture medium was selected suitable for lacasse production. To increase the production of lacasse using different inducers and to examine the ability of dechlorination, this article focuses on screen lacasse activity of 21 basidiomycetes isolates grown in five culture media. All inducers evaluated influenced lacasse activity positively except for gallic acid, mannitol, and malt extract for studied isolates. Our findings showed that lacasse activity of Trametes versicolor ATCC (200801) when it was induced with wheat bran reached up to 29.08 U ml -1 and was examined the ability of dechlorination of 2,4,5-trichlorophenol (2,4,5-TCP). The parameters including pH, initial substrate concentration, amount of enzyme, period of reaction, and temperature were tested for dechlorination process. Correlation between oxygen consumption and dechlorination processes under the determined optimum conditions was analyzed. Toxicity of 2,4,5-TCP before and after enzymatic treatment was evaluated by Microtox test. The results demonstrated that toxicity of intermediates formed 2,4,5-TCP did not change.

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Section: In This Study 20 Basidiomycetes Isolates Such As P Arcularmentioning

confidence: 83%

Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol

Gedikli¹,

Aytar²,

Ünal³

et al. 2010

Electron Journ of Biotechnol

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“…Additionally, it has been proposed that laccase induction stops when the glucose has been consumed and when compounds have been solvated or liberated during lignin degradation (Ha et al, 2001;Moldes et al, 2004). The results presented in Fig.…”

Section: Enzymatic Activitymentioning

confidence: 94%

VARIABILITY IN THE LIGNINOLYTIC ENZYMES ACTIVITY BY <I>LENTINULA EDODES</I> IN SUBMERGED CULTURE WITH LIGNIN AND GLUCOSE

Harris-Valle¹

2014

American Journal of Agricultural and Biological Sciences

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The white-rot basidiomycete Lentinula edodes is the second most edible mushroom cultivated on the world. This fungus decomposes cell-wall associated macromolecules, is a natural degrader of lignin polymers. The differences in enzyme activities between strains of L. edodes provided useful information about the participation of enzymes in different development stages of the fungus. The effect of lignin on the fungal biomass production and activity behavior of ligninolytic enzymes when L. edodes is cultivated in a medium containing lignin with and without glucose as a carbohydrate source was tested. When glucose was present in the culture, lignin increase the mycelial biomass by 70% at 22 days compared to the control culture. The lignin media without glucose affected mycelial growth up to 20% less that the control without lignin and glucose. The activity of laccase, lignin peroxidase, aryl alcohol oxidase, manganese dependent peroxidase and catalase was modified depending on whether the medium had lignin and glucose, or lignin alone. A carbohydrate source is important to fungal growth, but the dissolution of lignin monomers might switch the signal that controls growth rate and enzymatic activity.

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“…One of the most appropriate approaches to produce low cost and efficient enzymes for biotechnological application purposes is to utilize the potential of lignocellulosic waste materials. Some such mixtures may contain significant concentrations of soluble carbohydrates and inducers of enzyme synthesis so as to allow efficient production of ligninolytic enzymes (Reddy et al 2003, Moldes et al 2004Elisashvili et al 2006;Iqbal et al 2011a,b;Asgher et al 2012c). To date, the production of various ligninolytic enzymes including lignin peroxidase (LiP), manganese peroxidase (MnP), versatile peroxidase (VP), laccases, and other lignocellulytic enzymes, mainly cellulases, have been widely studied in submerged and solid culture processes in the laboratory, ranging from shake flask to a large scale (Xia and Len 1999;Moldes et al 2004;Elisashvili et al 2006).…”

Section: Production Of Enzymesmentioning

confidence: 99%

“…Some such mixtures may contain significant concentrations of soluble carbohydrates and inducers of enzyme synthesis so as to allow efficient production of ligninolytic enzymes (Reddy et al 2003, Moldes et al 2004Elisashvili et al 2006;Iqbal et al 2011a,b;Asgher et al 2012c). To date, the production of various ligninolytic enzymes including lignin peroxidase (LiP), manganese peroxidase (MnP), versatile peroxidase (VP), laccases, and other lignocellulytic enzymes, mainly cellulases, have been widely studied in submerged and solid culture processes in the laboratory, ranging from shake flask to a large scale (Xia and Len 1999;Moldes et al 2004;Elisashvili et al 2006). Due to the lower capital investment and operating costs of solid state fermentation (SSF), that approach has been reported as an attractive alternative process to produce fungal microbial enzymes using different agro-industrial based lignocellulosic materials (Jecu 2000;Couto and Sanromán 2005;Couto and Sanromán 2006;Levin et al 2008;Oberoi et al 2010;Iqbal et al 2011a,b).…”

Section: Production Of Enzymesmentioning

confidence: 99%

Advances in the Valorization of Lignocellulosic Materials by Biotechnology: An Overview

Iqbal¹,

Kyazze²,

Keshavarz³

2013

BioResources

183

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In view of the worldwide economic and environmental issues associated with the extensive use of petro-chemicals, there has been increasing research interest during the past decade in the value of residual biomass. Because of its renewable nature and abundant availability, residual biomass has attracted considerable attention as an alternate feedstock and potential energy source. To expand the range of natural bio-resources, significant progress related to the lignocellulose biotechnology has been achieved, and researchers have been re-directing their interests to biomass-based fuels, ligninolytic enzymes, chemicals, and biocompatible materials, which can be obtained from a variety of lignocellulosic waste materials. This review article focuses on the potential applications of lignocellulosic materials in biotechnology, including the production of bio-fuels, enzymes, chemicals, the pulp and paper, animal feed, and composites.

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Different proportions of laccase isoenzymes produced by submerged cultures of Trametes versicolor grown on lignocellulosic wastes

Cited by 81 publications

References 10 publications

Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol

Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol

VARIABILITY IN THE LIGNINOLYTIC ENZYMES ACTIVITY BY <I>LENTINULA EDODES</I> IN SUBMERGED CULTURE WITH LIGNIN AND GLUCOSE

Advances in the Valorization of Lignocellulosic Materials by Biotechnology: An Overview

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