Different preference is modulated by the feeding stimulants supplementation in different Chinese soft-shelled turtle (Pelodiscus sinensis ) basic diets

Abstract

Five 2-week feeding trials were conducted to investigate five stimulants on P. sinensis.Two isonitrogenous and isoenergetic diets were formulated, one contained 600 g kgfishmeal (FM diet) and the other contained 420 g kg -1 fishmeal and 225 g kg -1 animal protein blend (APB diet), and each tested one stimulant with four levels. The feeding stimulant candidates were betaine, 2-carboxy-ethyl dimethyl sulphonium bromide (DMPT), inosine-5′-monophosphate (IMP), taurine and squid extract. In the experiment on each stimulant, turtles (10.85 ± 0.10 g) were equally divided into FM and APB groups and fed with an equal mixture of corresponding diets containing four levels of stimulants, respectively. Each diet contained a unique rare earth oxide as inert marker.Turtles were fed twice daily (8:00 and 17:00), and faeces were collected. Preference for each diet was estimated based on the relative concentration of each marker in the faeces. In the FM group, only IMP showed the enhanced attraction. But in APB groups, all the stimulants, except IMP, showed higher preference than basal diets for at least one inclusion level, and the optimum level was 10 g kg -1 for betaine, 0.1 g kg -1 for DMPT, 0.1 g kg -1 for IMP, 5 g kg -1 for taurine and 10 g kg -1 for squid extract, and the squid extract had the strongest stimulating effect among the stimulants. K E Y W O R D Sanimal protein blend, Chinese soft-shelled turtle (Pelodiscus sinensis), feeding stimulants, fish meal replacement, taste preference

Dietary squid paste supplementation promotes feed intake via brain-gut dynamic response in Chinese soft-shelled turtle Pelodiscus sinensis

“…In the present study, turtles fed diets included squid paste presented lower peak mRNA level of CCK and CCK1R than that in other groups. It might be accounted for the improved activities of the digestive enzyme in the RPS group (Sun et al, 2018a;Sun et al, 2018b). Similar results were also found in grass carp (Liu et al, 2014;Huang et al, 2019).…”

“…RPS diet was different from the RP diet by the inclusion of 1% squid paste. This concentration of squid paste was determined by the optimal dietary preference of Pelodiscus sinensis (Sun et al, 2018a;Sun et al, 2018b). To maintain the consistencies of essential amino acid profiles in the experimental diets, microcapsule lysine was supplemented according to the essential amino acid profiles of the CT diet.…”

“…Three experimental feeds were randomly assigned to turtles with quadruplicate tanks. Management of the feeding trial was conformed to the method of Sun et al (2018a) and Sun et al (2018b). Feed pellets were put on a sedentary plate under the water.…”

Effects of garlic powder on feeding attraction activity, growth and digestive enzyme activities of Japanese seabass, Lateolabrax japonicus

“…taste and smell) to locate food (Velez et al, ). Reports presented that volatile organic acids, sugars, amino acids and sulphur‐containing organic compounds had significant influence in the feeding attraction activity of aquatic animals (Hara, ; Harada & Matsuda, ; Harada et al, ; Pan, Xian, Kou, Wang, & Miao, ; Sun et al, ; Xue & Cui, ; Zhang et al, ). In this study, medicinal plant garlic powder showed significant feeding attraction activity for Japanese seabass.…”

Transcriptome analysis and identification of genes and single‐nucleotide polymorphisms associated with growth traits in the Chinese soft‐shelled turtle, Pelodiscus sinensis

“…Understanding the molecular mechanisms of growth regulation and the genetic basis of growth variation may contribute to the genetic improvement of P. sinensis . Nowadays, many studies of P. sinensis in the fields of nutrition (Cheng, Huang, Wang, & Guanghong, 2017; Ji, Liu, Li, & Yang, 2017; Sun et al, 2018), immune (Lyu et al, 2020), and sex control (Cai et al, 2016; Sun et al, 2017) have been reported, while the molecular mechanisms involved in its growth are poorly understood.…”