Different Power Settings of LLLT on the Repair of the Calcaneal Tendon

Neves, Marco Aurélio Invaldi; Pinfildi, Carlos Eduardo; Wood, Viviane Timm; Gobbato, Rafael Corrêa; Silva, Fabio Mendes da; Parizotto, Nivaldo Antônio; Hochman, Bernardo; Ferreira, Lydia Masako

doi:10.1089/pho.2010.2919

Cited by 36 publications

(37 citation statements)

References 31 publications

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“…Group C: rats subjected to surgical procedures without tendon lesion and hAM application simulation; Group I: animals subjected to surgical procedures, including tendon injury, and hAM application simulation; and Group T: animals subjected to surgical procedures, including tendon injury, and hAM application. The tendon injury was induced by direct trauma in animals of groups I and T using a mini guillotine (Physiology Laboratory at the University of Paraíba Valley), according to the protocol used by Neves et al 14 . The sample size of 5 subjects per group was validated by calculating the power (beta), obtaining values of 0.86 for the inflammatory cell counts and 0.99 for fibroblast count.…”

Section: ■ Methodsmentioning

confidence: 99%

Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats

et al. 2017

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1 5-Experimental SurgeryActa Cir Bras. 2017;32(2):125-139 Abstract Purpose: To evaluate the effect of human amniotic membrane (hAM) fragment on inflammatory response, proliferation of fibroblast and organization of collagen fibers in injured tendon. Methods: Sixty rats were divided into 3 groups: C -surgical procedures without tendon lesion and with simulation of hAM application; I -surgical procedures, tendon injury and simulation of hAM application; T -surgical procedures, tendon injury and hAM application. These groups were subdivided into four experimental times (3, 7, 14 and 28 days). The samples underwent histological analysis and ATR-FTIR spectroscopy. Results: Histological analysis at 14 days, the T group showed collagen fibers with better alignment. At 28 days, the I group presented the characteristics described for the T group at 14 days, while this group presented aspects of a mature connective tissue. FT-IR analysis showed a clear distinction among the three groups at all experimental times and groups T and I presented more similarities to each other than to group C. Conclusion: Acute injury of tendon treated with human amniotic membrane fragment showed a faster healing process, reduction in inflammatory response, intense proliferation of fibroblasts and organization of collagen fibers.

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Section: ■ Methodsmentioning

confidence: 99%

Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats

et al. 2017

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“…It is important to note that this study utilized a constant power output and power density and only altered the time of treatment in order to study different energy density effects. It is unclear if a constant time with variable power density would have had the same outcome, as previous studies have shown that altering the power density can have significant biological effects . It is clear that both parameters are likely to have marked impact on outcome.…”

Section: Discussionmentioning

confidence: 99%

808 nm Wavelength Light Induces a Dose‐Dependent Alteration in Microglial Polarization and Resultant Microglial Induced Neurite Growth

et al. 2013

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“…Wood et al also found that ultrasound, LLLT, and the combined use of LLLT and ultrasound resulted in greater synthesis of type I collagen (but not type III collagen) [25]. Moreover, different power settings of low-level laser treatment might also contribute to the significantly differential expressions of type I and type III collagen [26]. Thus, we believed, under certain settings of laser parameters, low-level laser could predominantly affect the synthesis of type I collagen from tenocytes.…”

Section: Discussionmentioning

confidence: 99%

Second messengers mediating the proliferation and collagen synthesis of tenocytes induced by low-level laser irradiation

Chen¹,

Huang²,

Sun

et al. 2014

Lasers Med Sci

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For decades, low-level laser therapy (LLLT) has widespread applications in tendon-related injuries. Although the therapeutic effect of LLLT could be explained by photostimulation of target tissue and cells, how tenocytes sense photonic energy and convert them into cascades of cellular and molecular events is still not well understood. This study was designed to elucidate the effects of LLLT on cell proliferation and collagen synthesis by examining the associated second messengers including ATP, Ca(2+), and nitric oxide using rat Achilles tenocytes. Moreover, proliferating cell nuclear antigen (PCNA) and transforming growth factor-β1 (TGF-β1) related to cell proliferation and matrix metabolism were also studied. The results showed that 904 nm GaAs laser of 1 J/cm(2) could significantly increase the MTT activity and collagen synthesis of tenocytes. Second messengers including ATP and intracellular Ca2+ were increased after laser treatment. Quantitative PCR analysis of tenocytes treated with laser revealed up-regulated expression of PCNA, type I collagen, and TGF-β1. Besides, laser-induced TGF-β1 expression was significantly inhibited by extracellular signal-regulated kinase (ERK) specific inhibitor (PD98059). The findings suggested that LLLT stimulated ATP production and increased intracellular calcium concentration. Directly or indirectly via production of TGF-β1, these second messengers mediated the proliferation of tenocytes and synthesis of collagen.

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Different Power Settings of LLLT on the Repair of the Calcaneal Tendon

Abstract: Low-level laser therapy resulted in significantly greater amounts of type III collagen (output powers of 60 mW or more) and type I collagen (output power of 80 mW), however, no significant differences between groups were found in the realignment of collagen fibers.

Cited by 36 publications

References 31 publications

Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats

Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats

808 nm Wavelength Light Induces a Dose‐Dependent Alteration in Microglial Polarization and Resultant Microglial Induced Neurite Growth

Second messengers mediating the proliferation and collagen synthesis of tenocytes induced by low-level laser irradiation

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