Different populations of CD11b+ dendritic cells drive Th2 responses in the small intestine and colon

Abstract

T-helper 2 (Th2) cell responses defend against parasites. Although dendritic cells (DCs) are vital for the induction of T-cell responses, the DC subpopulations that induce Th2 cells in the intestine are unidentified. Here we show that intestinal Th2 responses against Trichuris muris worms and Schistosoma mansoni eggs do not develop in mice with IRF-4-deficient DCs (IRF-4f/f CD11c-cre). Adoptive transfer of conventional DCs, in particular CD11b-expressing DCs from the intestine, is sufficient to prime S. mansoni-specific Th2 responses. Surprisingly, transferred IRF-4-deficient DCs also effectively prime S. mansoni-specific Th2 responses. Egg antigens do not induce the expression of IRF-4-related genes. Instead, IRF-4f/f CD11c-cre mice have fewer CD11b+ migrating DCs and fewer DCs carrying parasite antigens to the lymph nodes. Furthermore, CD11b+CD103+ DCs induce Th2 responses in the small intestine, whereas CD11b+CD103− DCs perform this role in the colon, revealing a specific functional heterogeneity among intestinal DCs in inducing Th2 responses.

Fasting-Mimicking Diet Modulates Microbiota and Promotes Intestinal Regeneration to Reduce Inflammatory Bowel Disease Pathology

“…In the colon, we analyzed CD11b + dendritic cells. These types of dendritic cells can drive type 2 T-helper lymphocyte (TH2) responses, with the CD11b + CD103~ sub-population mediating these responses in the colon and the CD11b + CD103 + sub-population in the small intestine (Mayer et al, 2017). One study found increased numbers of CD11b + cells in the colonic lamina propria (LP) of mice predisposed to colitis (Ey et al, 2013).…”

“…Certain strains of Lactobacillus tend to suppress T H 2 responses, and because CD11b + cells have been observed to drive T H 2 responses in the colon. It is plausible from these results that Lactobacillus works to suppress TH2 responses in the colon by reducing CD11b + levels (Mayer et al, 2017). …”

Immune modulation of macrophage response by Cordyceps militaris extracts

“…or Th17 cells [26,29]. These results suggest that the cordycepin extract activates macrophages to synthesize suppressors of pro-in ammatory signals and enhances DC differentiation.…”

CD300f is a potential therapeutic target for the treatment of food allergy

“…Typically, CD103 + DCs in the small intestinal LP capture food antigens and migrate from the LP to the draining LN, where CD103 + DCs induce naïve T cells to differentiate into Tregs. 1,8 Interestingly, the percentages of CD103 + CD11b + DCs among MLN CD11c + DCs were higher in OVA-challenged WT mice after sensitization than in CD300f -/counterparts ( Figure 2J OVA/alum PBS OVA/alum OVA WT KO WT KO (n = 2) (n = 2) (n = 10) (n = 7)…”