Different patterns of p16INK4a immunohistochemical expression and their biological implications in laryngeal squamous cell carcinoma

Lazăr, C; Şovrea, Alina Simona; Georgiu, Carmen; Crişan, Dana; Mirescu, Ştefan Claudiu; Cosgarea, Marcel

doi:10.47162/rjme.61.3.08

Cited by 5 publications

(6 citation statements)

References 40 publications

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“…Increased p16 expression in the incidence of ameloblastoma can provide an overview of the severity of its role as a tumour-suppressor gene in inhibiting the uncontrolled proliferation process. 19,20 This study found a significant increase of p16 expression in both types of ameloblastomas compared to the healthy gingival tissue, with the highest expression of p16 shown in the plexiform type ameloblastoma.…”

Section: Discussionmentioning

confidence: 60%

Immunohistochemical differential expression of p16 proteins in follicular type and plexiform type ameloblastoma

Widodo

Saptiwulan

Hirawan

et al. 2022

Dent. J. (Majalah Kedokt. Gigi)

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Background: Differences in histopathological features that describe the growth mechanism and biological behaviour of follicular and plexiform ameloblastomas are associated with benign, aggressive and destructive tumour markers. p16 has inhibitory interactions between cyclin D and CDK 4/6 to block the cell cycle and alterations related to severity. Purpose: This study intends to evaluate and determine differential expressions of p16 protein in follicular and plexiform ameloblastomas. Methods: This is a descriptive analytics study. A total of 21 specimens consisting of follicular and plexiform ameloblastomas and healthy gingiva tissues as the negative control were examined using the immunohistochemistry assay. The analysis of p16 protein expression was interpreted by immunoreactive scoring. Statistical analysis was conducted using SPSS software with the Mann–Whitney test. A p-value <0.05 shows the significance of the change in expression. Results: An increased expression of p16 protein was found in the follicular ameloblastoma type (2.13 ± 1.808) and the plexiform type (4.44 ± 2.506) in comparison to the negative control group (0 ± 0). The increase of p16 expression in the follicular and plexiform ameloblastomas was significant compared to the negative control group (p-value <0.05); however, there was no significant difference between either type of ameloblastoma (p-value >0.05). Conclusion: The highest intensity of p16 protein expression was found in the plexiform type, even though it was not significantly different from the follicular type ameloblastoma.

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Section: Discussionmentioning

confidence: 60%

Immunohistochemical differential expression of p16 proteins in follicular type and plexiform type ameloblastoma

Widodo

Saptiwulan

Hirawan

et al. 2022

Dent. J. (Majalah Kedokt. Gigi)

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“…Fortunately, subsequent Aptima HPV and RNAscope detection revealed HPV negativity. To the best of our knowledge, there have been no reports of such strong nuclear staining of p16 since single cytoplasm staining has been reported [29,30]. Viral elimination is a normal response of the human immune system during the evolution of the disease, and protein expression decreases along with the reduction in pathogenic genes, resulting in the remaining protein being confined to the nucleus but not the cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

Aptima HR-HPV Testing of Cytology Specimens Is an Effective Supplement for p16 Staining to Improve Diagnostic Accuracy for HPV-Related Oropharyngeal Squamous Cell Carcinoma

Yang¹,

Hu²,

Zhao³

et al. 2022

Acta Cytologica

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Introduction: Regarding a small proportion of oropharyngeal squamous cell carcinoma (OPSCC) patients who tested p16-positive but human papillomavirus (HPV)-negative, we attempted to perform HPV testing to improve the accuracy of HPV detection in OPSCC patients. Methods: We simultaneously performed Aptima HPV testing of cytological specimens and p16 immunohistochemistry (IHC) staining of histologic biopsies from the same cohort of patients with head and neck SCC (HNSCC). The cytological specimens included fine-needle aspiration specimens from patients with enlarged nodes and endoscopic brushing specimens from the primary lesions of patients without enlarged nodes. Cases with discordant results for p16 IHC staining and Aptima HPV testing were reexamined by a third method, RNAscope testing. Results: Sixty patients with HNSCC (39 OPSCC and 21 non-OPSCC) were recruited for examination of HPV status. Among these patients, 28 were p16+/HPV+, 29 were p16−/HPV−, 2 were p16+/HPV−, and 1 was p16−/HPV+. The overall concordance rate between Aptima HPV testing and p16 IHC was 95.0%. Three cases with discordant results for these two methods were reexamined by RNAscope testing, and all were confirmed to be HPV negative. The prevalence of HPV in OPSCC and non-OPSCC patients was 61.5% (24/39) and 19.0% (4/21), respectively. The sensitivity and negative predictive values of Aptima HPV testing and p16 IHC were consistent at 100%, while the specificity and positive predictive values were 96.9% and 96.6% versus 93.8% and 93.3%, respectively. Additionally, 30 OPSCCs were simultaneously examined and diagnosed by both brush cytology and biopsy pathology; six of these SCCs were underdiagnosed by histopathology but accurately diagnosed by supplemental brush cytology. Conclusion: Aptima HPV testing of cytology specimens can be used as an adjuvant examination to identify false-positive OPSCC patients after p16 IHC of biopsies, while brush cytology may be a supplemental method for the histologic diagnosis of malignant oropharyngeal tumors.

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“…Elhadj et al show that the expression positive of p16 INK4a was found in 36 (51.43%) of 70 LSCC cases [ 37 ]. Expression of p16 INK4a might be affected by genetic or epigenetic mechanisms [ 38 ]. Some studies suggest hypermethylation may represent an early event in carcinogenesis of different neoplasms, such as endometrial cancer [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

p16INK4a and pRb expression in laryngeal squamous cell carcinoma with and without infection by EBV or different genotypes of HPV: a retrospective study

Vazquez-Guillen,

Palacios-Saucedo,

Alanis-Valdez

et al. 2023

Infect Agents Cancer

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Background Laryngeal squamous cell carcinoma (LSCC) represents one of the principal tumors of the head and neck. Human papillomavirus (HPV) and Epstein–Barr virus (EBV) are considered risk factors for the development and the clinical prognosis of LSCC. High levels of p16INK4a are suggested as a surrogate marker of HPV or EBV infection in some head and neck tumors but in LSCC is still controversial. Furthermore, pRb expression may be considered an additional biomarker but it has not been clearly defined. This work aimed to compare the expression of pRb and p16INK4a as possible biomarkers in tumor tissues with and without infection by EBV or different genotypes of HPV from patients with LSCC. Methods Tumor samples from 103 patients with LSCC were previously investigated for the presence and genotypes of HPV using the INNO-LiPA line probe assay and for the infection of EBV by qPCR. p16 INK4a and pRb expression was assessed by immunohistochemistry. Results Of the 103 tumor samples, expression of p16INK4a was positive in 55 (53.4%) and of this, 32 (56.1%) were positive for HPV whereas 11 (39.3%) were EBV positive but both without a significantly difference (p > 0.05). pRb expression was positive in 78 (75.7%) and a higher frequency of this expression was observed in HPV negative samples (87.0%) (p = 0.021) and in high-risk HPV negative samples (85.2%) (p = 0.010). No difference was observed when comparing pRb expression and EBV infection status (p > 0.05). Conclusion Our results support the suggestion that p16INK4a is not a reliable surrogate marker for identifying HPV or EBV infection in LSCC. On the other hand, most of our samples had pRb expression, which was more frequent in tumors without HPV, suggesting that pRb could indicate HPV negativity. However, more studies with a larger number of cases are required, including controls without LSCC and evaluating other molecular markers to determine the real role of p16INK4a and pRb in LSCC.

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Different patterns of p16INK4a immunohistochemical expression and their biological implications in laryngeal squamous cell carcinoma

Cited by 5 publications

References 40 publications

Immunohistochemical differential expression of p16 proteins in follicular type and plexiform type ameloblastoma

Immunohistochemical differential expression of p16 proteins in follicular type and plexiform type ameloblastoma

Aptima HR-HPV Testing of Cytology Specimens Is an Effective Supplement for p16 Staining to Improve Diagnostic Accuracy for HPV-Related Oropharyngeal Squamous Cell Carcinoma

p16INK4a and pRb expression in laryngeal squamous cell carcinoma with and without infection by EBV or different genotypes of HPV: a retrospective study

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