Different pathways of differentiation of pre-B cell lines are induced by dendritic cells and T cells from different lymphoid tissues

Spalding, David M.; Griffin, Johanna A.

doi:10.1016/0092-8674(86)90472-1

Cited by 100 publications

(36 citation statements)

References 34 publications

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“…Also, IL-3-sensitive pre-B-cell lines have been obtained from the bone marrow and spleen of young adult mice (32,37,42,46). Normal human pro-B and pre-B cells as well as their leukemic counterparts have also been found to proliferate in IL-3 (21,48,49,53).…”

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confidence: 99%

Fetal liver pro-B and pre-B lymphocyte clones: expression of lymphoid-specific genes, surface markers, growth requirements, colonization of the bone marrow, and generation of B lymphocytes in vivo and in vitro.

Palacios¹,

Samaridis²

1992

Mol. Cell. Biol.

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We describe here the development and characterization of the FLS4.1 stromal line derived from 15-day fetal liver of BALB/c embryos and defined culture conditions that efficiently support the cloning and long-term growth of nontransformed B-220+ 14-day fetal liver cells at two stages of B-cell development, namely, pro-B lymphocytes (immunoglobulin [Ig] genes in germ line configuration) and pre-B cells (JH-rearranged genes with both light-chain Ig genes in the germ line state). All B-cell precursor clones require recombinant interleukin-7 (rIL-7) and FLS4.1 stromal cells for continuous growth in culture, but pro-B lymphocyte clones can also proliferate in rIL-3. None proliferate in rIL-i, rIL-2, rIL-4, rIL-5, rIL-6, or leukemia inhibitory factor. FLS4.1 stromal cells synthesize mRNA for Steel factor but not for IL-1 to IL-7; all pro-B and pre-B clones express c-Kit, the receptor for Steel factor, and a c-Kit-specific antibody inhibits the enhanced proliferative response of fetal liver B-220+ B-cell precursors supported by FLS4.1 stromal cells and exogenous rIL-7 but does not affect that promoted by rIL-7 alone. Northern (RNA) blot analysis of the expression of the MB-1, A5, Vpre-B, c,u, RAG-1, and RAG-2 genes in pro-B and pre-B clones show that transcription of the MB-1 gene precedes IgH gene rearrangement and RNA synthesis from c,, RAG-1, RAG-2, A5, and Vpre-B genes. All clones at the pre-B-cell stage synthesize mRNA for c,u, RAG-1, and RAG-2 genes; transcription of the AS and Vpre-B genes seems to start after D-to-JH rearrangement in B-cell precursors, indicating that the proteins encoded by either gene are not required for B-cell progenitors to undergo D-to-JH gene rearrangement. These findings mark transcription of the MB-1 gene as one of the earliest molecular events in commitment to develop along the B-lymphocyte pathway. Indeed, both pro-B and pre-B clones can generate in vitro and in vivo B lymphocytes but not T lymphocytes; moreover, these clones do not express the CD3--y T-cell-specific gene, nor do they have rearranged y, 8, or j8 T-cell antigen receptor genes.

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mentioning

confidence: 99%

Fetal liver pro-B and pre-B lymphocyte clones: expression of lymphoid-specific genes, surface markers, growth requirements, colonization of the bone marrow, and generation of B lymphocytes in vivo and in vitro.

Palacios¹,

Samaridis²

1992

Mol. Cell. Biol.

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“…It is also likely the initial site for the generation of regulatory T cells producing IL-10 and TGF-β after low dose antigen feeding. In addition, prior studies suggested that dendritic cells derived from Peyer's patch macrophages may be unique in their ability to prime T cells for providing help for IgA B cell differentiation [39]. However, not all oral encounters with antigens result in tolerance.…”

Section: Yield Purity and Viability Of The Generated Dendritic Cellsmentioning

confidence: 99%

“…), MHC class II low , CD86 low (B7-2 low ) DPP-DC population from T. gondii infected C57BL/6J mice. The precise role of dendritic cell (DC) [39] populations in regulating the immune responses to orally administered antigens remains unclear. We have focused our studies on the phenotype of DC derived from discrete Peyer's patch macrophages, as this lymphoid tissue is the primary site for the induction of mucosal immune responses, such as the differentiation of B cells capable of producing secretory IgA.…”

Section: Yield Purity and Viability Of The Generated Dendritic Cellsmentioning

confidence: 99%

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A Comparison of the Phenotype of Dendritic Cells Derived from Discrete Peyer's Patch Macrophages of Non-Infected and Toxoplasma Gondii Infected Mice

Makala

Reyes

Nishikawa

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. A comparison of the expression of surface membrane antigens between dendritic cells (DC) derived from Peyer's patch macrophages (DPP-DC) of non-infected and Toxoplasma gondii (T. gondii) infected mice was performed. C57BL/6J mice aged 6-8 weeks of both sexes were infected orally with a 0.5 ml suspension containing 2 × 10 4 bradyzoites of the Beverley strain of T. gondii, sacrificed on day 8 and DC generated using discrete Peyer's patch macrophages (DPP-Mø) as progenitor cells. When a comparison of the expression of surface membrane antigens between the antigen presenting cells (APC) obtained from discrete Peyer's patches of non-infected and T. gondii infected mice was carried out, no significant differences were observed in the macrophage progenitor and DC populations expression of F4/80, DEC-205, CD11c, CD80 (B7-1) and CD34. However, a significant decrease in MHC class II antigen levels and a down regulation of the co-stimulatory molecule CD86 (B7-2) were noted. B7-1 appeared to be the dominant co-stimulatory ligand, whereas B7-2, which was down regulated during T. gondii infection, had a weak expression. Taken together, these results may help clarify the role of DC in the complex network regulating surface membrane antigens, as well as, their capacity for antigen uptake, processing and presentation during toxoplasmosis. Dendritic cells (DC) represent a rare population of antigen presenting cells (APC) in the blood, non-lymphoid and lymphoid tissues. They play a key role in the onset of cellular immunity and many studies have shown that DC capture, process and present antigen to memory and naive T cells [24][25][26][27]30]. The function of DC can be characterized by the dynamic regulation of differentiation/activation markers (CD83, CD25), of co-stimulatory molecules (CD40, CD80, CD86), and of class II major histocompatibility complexes (MHC class II) [14, 26-29, 31, 34].The major mechanism by which immuno-competent hosts control Toxoplasma gondii (T. gondii) infection is considered to be cell-mediated immunity [12]. The microbicidal or micro-biostatic activity of activated APC [40] and non-phagocytic cells [6,43] are the two major mechanisms of resistance to T. gondii infection. The physiologic regulation of Th phenotype development is still poorly understood, but because of the MHC class II restriction, attention has been focused on the major role of APC in the initiation of the immune response. In vitro studies have shown that activation of Th clones requires the presence of particular APC, i.e., DC or macrophages [8,33].High doses of the same soluble protein antigen given orally can result in non-responsiveness due to deletion or anergy of antigen-specific T cells [8], similar to what occurs following systemic administration of soluble proteins [33].However, not all oral encounters with antigen result in tolerance. Local and systemic T cell priming can occur when soluble antigens are administered with an ADP-ribosylating adjuvant, such as cholera toxin, which results in a Th2-dominant response, or...

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“…Control of class switching by T cells specific for residue epitopes on processed translation products of unrearranged constant-region gene seg ments might also be possible, since these gt:tne segments remain tran scriptionally active after the formation of a f.1-chain gene (9,92).…”

Section: A Possible Mechanism For Network Control Of B-cell and T-celmentioning

confidence: 99%

Crime and Punishment in the Society of Lymphocytes: A Speculation on the Structure of the Putative Idiotypic Network

Cleveland¹

1988

Anti-Idiotypes, Receptors, and Molecular Mimicry

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Different pathways of differentiation of pre-B cell lines are induced by dendritic cells and T cells from different lymphoid tissues

Cited by 100 publications

References 34 publications

Fetal liver pro-B and pre-B lymphocyte clones: expression of lymphoid-specific genes, surface markers, growth requirements, colonization of the bone marrow, and generation of B lymphocytes in vivo and in vitro.

Fetal liver pro-B and pre-B lymphocyte clones: expression of lymphoid-specific genes, surface markers, growth requirements, colonization of the bone marrow, and generation of B lymphocytes in vivo and in vitro.

A Comparison of the Phenotype of Dendritic Cells Derived from Discrete Peyer's Patch Macrophages of Non-Infected and Toxoplasma Gondii Infected Mice

Crime and Punishment in the Society of Lymphocytes: A Speculation on the Structure of the Putative Idiotypic Network

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