Different Particle Types in Tissue Culture and Intestinal Epithelium Infected with Rotavirus

Chasey, D.

doi:10.1099/0022-1317-37-3-443

Cited by 38 publications

(24 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tubular structures have been observed in thin sections of infected cells both in the cytoplasm and in the nuclei and they may be identical to those seen in negatively stained preparations (Banfield et al, 1968;Suzuki & Konno, 1975;Saifet al, 1978;Pearson & McNulty, 1979;Rodriguez-Toro, 1980;Suzuki et al, 1981). It is clear, however, that the complexity of rotavirus morphogenesis (Chasey, 1977;Esparza et al, 1980;Petrie et al, 1981) is reflected also in the variety of related aberrant tubular structures, although the characterization of the protein components of rotatubes is a necessary prerequisite for their formal identification as specific components of the rotavirus capsid.…”

Section: Discussionmentioning

confidence: 97%

Electron Microscopy of Tubular Assemblies Associated with Naturally Occurring Bovine Rotavirus

Chasey¹,

Labram²

1983

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 97%

Electron Microscopy of Tubular Assemblies Associated with Naturally Occurring Bovine Rotavirus

Chasey¹,

Labram²

1983

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

“…These virus particles sometimes ap pear to bud through ribosome-free areas of the rough endoplasmic reticulum into swollen vesicles, becoming enveloped in the process. The diameter of the enveloped particles has been reported to range from 70 nm [10,12] to 90 nm [8,16], Large numbers of nonenveloped virus particles (62-70 nm) can also be found in cisternae of the rough endoplasmic reticu lum. It is not presently known whether the nonenveloped particles within theendoplasmic reticulum are derived from breakdown of en veloped particles or whether they enter the endoplasmic reticulum by some means other than budding through the membrane.…”

mentioning

confidence: 99%

“…One of these, designated as type IV particles by Chasey [10], is 50-65 nm in diameter. These particles are found in large vacuoles, together with cellular debris.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Identification of Rotavirus Particle Types

Petrie¹,

Graham²,

Estes³

1981

Intervirology

View full text Add to dashboard Cite

Negative-contrast electron microscopy of purified rotavirus particles reveals two particle types: single-shelled and double-shelled particles. The relationship of these particle types, seen by negative staining, to the enveloped and various types of nonenveloped particles seen in thin sections of virus-infected cells was determined. Thin-section and negative-contrast electron microscopic analyses were performed on cell lysates from simian rotavirus SA11-infected cells and on highly purified double- and single-shelled particles. In thin sections, double-shelled particles appeared as smooth-edged ovals containing dense nucleoids, whereas single-shelled particles had ragged edges and threads of material extending from their centers. The majority of nonenveloped particles seen in thin sections of infected cells were identified as double-shelled particles. Enveloped particles showed typical membrane structure and were observed rarely in crude rotavirus stocks, although they constitute about 10% of the particles within infected cells. It is hypothesized that the enveloped form is a transient one and the envelope is lost in the endoplasmic reticulum of the host cell. Finally, the 50–55 nm type IV particles seen within lysosome-like bodies in infected cells were identified as subviral particles formed from input virions.

show abstract

“…Single-shelled particles, 50 to 65 nm in diameter, were found within large membrane-bound inclusions or vacuoles and, although containing electron-dense cores, were distinct from common rotavirus particle types and intermediate forms. The infectivity of these 'defective' particles, designated Type IV (Chasey, 1977), was not established.…”

Section: Discussionmentioning

confidence: 99%

Replication of Atypical Ovine Rotavirus in Small Intestine and Cell Culture

Chasey¹,

Banks²

1986

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

SUMMARYColostrum-deprived lambs experimentally infected with an atypical ovine rotavirus isolated from naturally scouring animals, and a naturally infected colostrum-deprived lamb, were examined by immunofluorescence and immunoperoxidase labelling, and electron microscopy. A hyperimmune serum to the virus was produced in a gnotobiotic lamb and used to demonstrate antigen in the villous epithelial cells of the small intestine from infected animals. Scanning and transmission electron microscopy of tissues from infected animals revealed giant multinucleate syncytia composed of fused epithelial cells. Infected cells contained intracytoplasmic virus particles which resembled group A rotaviruses in morphology and morphogenesis. Small numbers of infected cells in MA104 cultures inoculated with ovine atypical rotavirus could be detected by immunofluorescence but virus growth could not be maintained by passage. Virus particles were seen by thin-section electron microscopy but their morphology and morphogenesis were abnormal; they were each composed of a single electron-dense shell, with no core, and were associated with envelopes of smooth membrane rather than rough endoplasmic reticulum.

show abstract

Different Particle Types in Tissue Culture and Intestinal Epithelium Infected with Rotavirus

Cited by 38 publications

References 10 publications

Electron Microscopy of Tubular Assemblies Associated with Naturally Occurring Bovine Rotavirus

Electron Microscopy of Tubular Assemblies Associated with Naturally Occurring Bovine Rotavirus

Identification of Rotavirus Particle Types

Replication of Atypical Ovine Rotavirus in Small Intestine and Cell Culture

Contact Info

Product

Resources

About