Different opinions on classification of DNA histograms produced from paraffin‐embedded tissue

Joensuu, Heikki; Kallioniemi, Olli

doi:10.1002/cyto.990100607

Cited by 66 publications

(23 citation statements)

References 15 publications

(6 reference statements)

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“…Although DNA flow cytometry has been claimed to be an objective method, the DNA histograms are not always similarly interpreted, resulting in a different percentage of DNA aneuplody found even if the data is similar (Joensuu & Kallioniemi, 1989). The use of DI abolishes some of the problems involved in data interpretation, since near diploid tumours and tumours with a small DI may be grouped together with the diploid tumours.…”

Section: Discussionmentioning

confidence: 99%

The prognostic significance of nuclear DNA content in invasive breast cancer – a study with long-term follow-up

Toikkanen

Joensuu²,

Klemi³

1989

Br J Cancer

117

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Summary The nuclear DNA content of 351 breast carcinomas was determined by flow cytometry from paraffin-embedded tissue to assess the prognostic significance of DNA ploidy, the DNA index (DI) and the S-phase fraction (SPF). The minimum follow-up of the patients was 22 years, and they were all from a defined urban population. DNA ploidy correlated with histological type and grade, mitotic count and nuclear pleomorphism (P<0.0001), and also with axillary nodal status (P=0.0005), tumour necrosis (P=0.001), primary tumour size (P = 0.03), menopausal status (P = 0.004) and the presence of distant metastases at the time of the diagnosis (P = 0.04). Survival corrected for intercurrent deaths of the patients with a diploid tumour was better than that of the patients with a non-diploid tumour (P = 0.0001, 48% vs 28% at 25 years). SPF Leatham & Brooks, 1987; Slamon et al., 1987).The nuclear DNA content determined by flow cytometry has recently emerged as a new prognostic factor in breast cancer. The technique has gained some popularity, since flow cytometric histograms are rapid to produce and often easy to interpret. It has been suggested that breast carcinomas with an abnormal (non-diploid or aneuploid) nuclear DNA content are associated with less favourable prognosis than carcinomas with a normal (diploid) DNA content (Hedley et al., 1984(Hedley et al., , 1987Coulson et al., 1984;Ewers et al., 1984;Thorud et al., 1986;Baildam et al., 1987;Cornelisse et al., 1987;Kallioniemi et al., 1987;Stal et al., 1989). Similarly, the DNA index (DI, the relative DNA content of an aneuploid stemline of cells as compared with diploid cells) and the percentage of S-phase cells in the DNA histogram (S-phase fraction, SPF) have been found by some to be of prognostic significance (Dowle et al., 1987;Hedley et al., 1987;Kallioniemi et al., 1988;Stal et al., 1989;McDivitt et al., 1986;Klintenberg et al., 1986). However, the prognostic value of nuclear DNA content analysis is still unsettled. Some authors report DNA content to be the most important prognostic variable in breast cancer, and to have independent prognostic value in a multivariate analysis , whereas others find it to have prognostic value only in a univariate analysis (Stal et al., 1989;Hedley et al., 1984), and yet a few fail to find any prognostic significance at all when overall survival is concerned (van der Linden et al., 1989;Uyterlinde et al., 1988;Owainati et al., 1987

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Section: Discussionmentioning

confidence: 99%

The prognostic significance of nuclear DNA content in invasive breast cancer – a study with long-term follow-up

Toikkanen

Joensuu²,

Klemi³

1989

Br J Cancer

117

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“…The frequency of aneuploidy in the nondysplast ic tissue is thus influ enced greatly by whether one interprets the near diploid peaks as aneuploidy. Interobserver variation in the interpre tatio n of near diploid peaks has been no ted in other tissues (9). This difference may well account for much of the variabili ty in the literatu re c ited above.…”

Section: Flow Cytometric Analysis Of Dna Content In Chronic Ulcerativmentioning

confidence: 97%

Carcinogenesis in Chronic Ulcerative Colitis: Flow Cytometry

McHugh

Bozdech

et al. 1990

Canadian Journal of Gastroenterology

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Flow cytometry is a conceptually straightforward quantitative method that can be used to determine the DNA content (and many ocher parameters) of individual cells. It has been used in chronic ulcerative colitis to evaluate the presence of aneuplo idy as a marker of colon cancer risk. In this paper, the basic principles offlow cytometry are reviewed, the current status of published studies using flow cytometric analysis of DNA content in chronic ulcerative colitis outlined, conclusions from the research are proposed and future research needs it~cntified. Overall, aneuploidy correlates with the current best markers of cancer risk in ulcerative colitis, ie, dysplasia and established cancer. Whether aneuplo1dy will be useful for the prospective identification of individuals at increased risk for colon cancer deserves rigorous evaluation. An important technical issue of potential variability in interpretation of near diplo id peak5 is emphasized. Can J Gastroenterol 1990;4(7):390-396 Key Words: Cancer, Flow cycometry, Surveillance, Ulcerarit1e colitis La carcinogenese dans la colite ulcereuse chronique et la cytometrie de flux RESUME: La cytometrie de flux est une methode quantitative simple pouvant servir a determiner le contenu d'ADN (parmi de nombreux parametres) des cellules individuelles. Elle permet de reperer l'aneuplo'idie en tant que marqueur de risque de cancer du colon dans la co lite ulcereuse chronique. Le present article passe en revue les principes elementaires de la cytometrie de flux, evalue le statue present des etudes publiees dans le domaine, presence leun, conclusions et definit les futurs besoins de la recherche. En general, ii ya correlation entre l'aneuplo'idie et les meilleurs indices de risque de cancer clans la colite ulcereuse, c'est-a-dire la dysplasie et le cancer etabli.11 est necessaire de proceder a un examen rigoureux afin de determiner si l'aneuplo'idie permettra de determiner qui presentent un risque eleve de cancer colique. L'auteur souleve une question technique importante: celle de la variabilite potentielle d'interpretation des pies proches de l'etat diplo'ide.

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“…A third group of publications describes inter-laboratory comparisons and focuses on the reproducibility of DNA flow cytometric results (4,(8)(9)(10)(11)13,18,23,24,26,28,37,41,44,49,50). The laboratories that were compared showed differences in the preparation and staining protocols, the kinds of flow instruments, and the interpretations of the histograms.…”

mentioning

confidence: 99%

“…Inconsistencies in ploidy and S-phase analysis appeared, even though most of the reports used only cultured cell lines and normal standard cells (which are easier to analyze than clinical tumor biopsy specimens). Instrumental drift and lack of consistency of preparation and staining (50) and the use of different criteria for tetraploidy, near-diploid aneuploidy, and small extra peaks (4,26) were suggested as possible explanations.…”

mentioning

confidence: 99%

Comparison of routine flow cytometric DNA analysis of fresh tissues in two laboratories: Effects of differences in preparation methods and background models of cell cycle calculation

et al. 1998

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Different opinions on classification of DNA histograms produced from paraffin‐embedded tissue

Cited by 66 publications

References 15 publications

The prognostic significance of nuclear DNA content in invasive breast cancer – a study with long-term follow-up

The prognostic significance of nuclear DNA content in invasive breast cancer – a study with long-term follow-up

Carcinogenesis in Chronic Ulcerative Colitis: Flow Cytometry

Comparison of routine flow cytometric DNA analysis of fresh tissues in two laboratories: Effects of differences in preparation methods and background models of cell cycle calculation

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