Different Nebulized Budesonide Dosing Regimens in a Mouse Model of Chronic Asthma

Abstract

Objective: The aim of this study was to compare the effectiveness of different inhaled steroid regimens on the lungs and their potential side effects on the bone tissues in chronic asthma model. Materials and Methods: Thirty-five specific pathogen-free BALB/c mice were divided into five groups. The mice in all of the study groups except the control group were sensitized with chicken egg albumin. After sensitization, the mice in group 2 were treated with saline modeling twice daily, the mice in group 3 were treated with 250 mcg of nebulized budesonide twice daily, the mice in group 4 were treated with 500 mcg of budesonide once daily, and the mice in group 5 were treated with 1000 mcg of budesonide every other day for the last 14 days of the challenge period. After challenge, the mice were sacrificed and lung and tibia samples were histologically examined. Results: Pulmonary parameters, including subepithelial smooth muscle thickness, goblet cell count, mast cell count and epithelial thickness, were the lowest in group 5 compared to other groups (p<0.01). Lung epithelial basement membrane thickness was the lowest in group 4 (p<0.01). In tibia bone tissue, there were no significant differences among treatment groups (group 3, 4 and 5) in terms of trabecular, cortical and osteoid thickness and number of trabeculae (p>0.01). Conclusion: The beneficial effect on lung tissue was highest in the treatment group receiving budesonide every other day (group 5) and no further measureable side effects on bone mineralization were observed in this group compared with the other treatment groups. Every-other-day treatment application seems to be the most effective regimen in chronic asthma model. Amaç: Çalışmanın amacı kronik astım modelinde farklı inhale steroid doz rejimlerinin akciğerler üzerine etkisinin ve kemikler üzerine olan yan etkisinin araştırılmasıdır. Gereç ve Yöntemler: Spesifik patojen içermeyen 35 BALB/c fare beş gruba ayrıldı. Kontrol grubu hariç diğer tüm gruplar tavuk yumurtası albumini ile duyarlaştırıldı. Duyarlanmadan sonra, grup 2'deki farelere günde iki kez salin, grup 3'deki farelere günde iki kez 250 mcg budezonid, grup 4'deki farelere günde tek doz 500 mcg nebul budezonid ve grup 5'deki farelere de iki günde bir 1000 mcg budezonid ilaç yükleme döneminin son 14 gününde uygulandı. İlaç uygulaması sonrasında fareler sakrifiye edilerek, akciğer ve tibia dokusu histolojik olarak incelendi.