Different Molecular Mechanisms Account for Drought Tolerance in Coffea canephora var. Conilon

Vieira, Natália Gomes; Carneiro, Fernanda A; Sujii, Patrícia Sanae; Alekcevetch, Jean Carlos; Freire, Luciana Pereira; Vinecky, Felipe; Elbelt, Sonia; Silva, Vânia Aparecida; DaMatta, Fábio M.; Ferrão, Maria Amélia Gava; Marraccini, Pierre; Andrade, Alan Carvalho

doi:10.1007/s12042-013-9126-0

Cited by 26 publications

(32 citation statements)

References 40 publications

(59 reference statements)

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“…The increased transcriptional activity of CcGolS1 under moderate and (particularly) severe stress was greater in D T leaves compared to D S leaves. Vieira et al (2013) have also observed different regulatory responses to drought stress in D T plants.…”

Section: Discussionmentioning

confidence: 94%

“…The drought stress experiment was done at the Agronomic Institute of Paraná (IAPAR – latitude 23°18′ S, longitude 51°09′ O, average altitude 585 m; Londrina, Paraná State, Brazil). Two C. canephora clones, D T (clone 14) and D S (clone 109A), with contrasting responses to water deficit stress ( DaMatta et al , 2003 ; Pinheiro et al , 2004 ; Marraccini et al , 2012 ; Vieira et al , 2013 ) were obtained from the Espírito Santo State Institute of Research, Technical Assistance and Rural Extension (Incaper, Brazil) and grown under greenhouse conditions. The experiments were done twice with four biological replicates per experiment.…”

Section: Methodsmentioning

confidence: 99%

“…Several clones of C. canephora ‘Kouillou’ (known in Brazil as ‘Conilon’) tolerant to drought have been identified in the germoplasm collection of the Institute for Research and Rural Assistance of Espírito Santo State (INCAPER), Brazil ( Ferrão et al ., 2000a ). Some of these accessions have been well characterized with regard to the physiological and molecular mechanisms involved in their tolerance to water deficit ( DaMatta et al ., 2003 ; Pinheiro et al , 2005 ; Praxedes et al ., 2006 ; Marraccini et al , 2012 ; Vieira et al , 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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Galactinol synthase transcriptional profile in two genotypes of Coffea canephora with contrasting tolerance to drought

et al. 2015

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Increased synthesis of galactinol and raffinose family oligosaccharides (RFOs) has been reported in vegetative tissues in response to a range of abiotic stresses. In this work, we evaluated the transcriptional profile of a Coffea canephora galactinol synthase gene (CcGolS1) in two clones that differed in tolerance to water deficit in order to assess the contribution of this gene to drought tolerance. The expression of CcGolS1 in leaves was differentially regulated by water deficit, depending on the intensity of stress and the genotype. In clone 109A (drought-susceptible), the abundance of CcGolS1 transcripts decreased upon exposure to drought, reaching minimum values during recovery from severe water deficit and stress. In contrast, CcGolS1 gene expression in clone 14 (drought-tolerant) was stimulated by water deficit. Changes in galactinol and RFO content did not correlate with variation in the steady-state transcript level. However, the magnitude of increase in RFO accumulation was higher in the tolerant cultivar, mainly under severe water deficit. The finding that the drought-tolerant coffee clone showed enhanced accumulation of CcGolS1 transcripts and RFOs under water deficit suggests the possibility of using this gene to improve drought tolerance in this important crop.

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Section: Discussionmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Galactinol synthase transcriptional profile in two genotypes of Coffea canephora with contrasting tolerance to drought

et al. 2015

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“…Consequently, many climate related experimental studies on coffee plants have focused on the effects of reduced water availability on coffee physiology, gene expression, and biochemical composition. For example, Marraccini et al (2012) and Vieira et al (2013) compared variations in the physiological and gene expression responses of drought tolerant and drought sensitive Robusta varieties, and suggested that the drought tolerant clones had subtle differences in ABA signaling and a better induction of the coffee antioxidant and osmoprotection systems. In another examination of the physiological effects induced by drought, (Menezes-Silva et al 2017) explored the effect of repeated rounds of water deficit and recovery on one Robusta variety.…”

Section: Introductionmentioning

confidence: 99%

Temperature Impacts the Response of Coffea canephora to Decreasing Soil Water Availability

Thioune

Strickler

Gallagher

et al. 2020

Tropical Plant Biol.

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Climate change is expected to result in more frequent periods of both low rainfall and above normal temperatures for many coffee growing regions. To understand how coffee reacts to such change, we studied the physiological and gene expression responses of the clonal variety C. canephora FRT07 exposed to water deficits under two different temperature regimes. Variations in the timedependent impact of water deficits on leaf stomatal conductance and carbon assimilation were significantly different under the 27°C and 27°C/42°C conditions examined. The physiological responses 24 h after re-watering were also different for both conditions. Expression analysis of genes known to respond to water deficits indicated that drought-related signaling occurred at both temperatures. Deeper insights into the response of coffee to water deficits was obtained by RNASeq based whole transcriptome profiling of leaves from early, late, and recovery stages of the 27°C experiment. This yielded expression data for 13,642 genes and related differential expression analysis uncovered 362 and 474 genes with increased and decreased expression, respectively, under mild water deficits, and 1627 genes and 2197 genes, respectively, under more severe water deficits. The data presented, from a single clonal coffee variety, serves as an important reference point for future comparative physiological/ transcriptomic studies with clonal coffee varieties with different sensitivities to water deficits and high temperatures. Such comparative analyses will help predict how different coffee varieties respond to changing climatic conditions, and may facilitate the identification of alleles associated with high and low tolerance to water deficits, enabling faster breeding of more climatesmart coffee trees.

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“…Dentre os genes avaliados, está presente a Dehidrina, que foi encontrada em bibliotecas de cDNA tanto de folha quanto de fruto (Vieira et al, 2013;Mondego et al, 2011). As…”

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Identificação e caracterização de peptídeos opioides presentes na fração proteica dos grãos de Coffea arabica

Vinecky¹

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AgradecimentosPrimeiramente agradeço à família que constituí durante o doutorado, minha esposa Michelle que me estimulou e me deu todo o apoio em todos os momentos, desde a graduação, no mestrado e principalmente durante o doutorado e pelo amor incondicional. Aos meus filhos Nina e Vitor que fizeram-me crescer mais, enxergar a vida com outros olhos e por cada sorriso diário.Agradeço à minha família iniciando pela minha irmã Verônica que também me estimulou, ajudou, cuidou de minha saúde, sempre esteve presente e solícita e no final ajudou muito com a correção de português da minha tese!!! À minha mãe que esteve do meu lado quando precisei, encorajamento, ajudando com as crianças quando precisava, pelos nossos momentos de descontração (nazdraví) e apoio. Ao meu pai por me ensinar que na vida precisamos aprender, pelo estímulo, a credibilidade em mim, toda a estrutura que me proporcionou desde sempre. À minha irmã Bárbara (e família) que sempre ajudou quando precisei, em todos os sentidos e principalmente com as crianças.Aos meus amigos, Eder que esteve presente em todos os momentos (inclusive antes do doutorado) desde a escrita do projeto, a execução e término do doutorado, às saídas de campo ora para pegar perereca mas também para pescaria, sinuca, foguinho... Minha amiga Mariana que esteve presente sempre, fazendo as coisas acontecerem, ajudando, trabalhando em equipe e sempre alegre! Meu amigo Zé (vulgo José de Lima) por participar das noites de "trabalho" que se tornavam diversão, pelas prosas e pela amizade! Ao amigo Daniel Sifú, que também participou do trabalho com ensinamentos e análises, pelas conversas e confiança. Aos amigos Yuri, André, Polyana, que mesmo de fora sempre foram solícitos, amigos de verdade. E tantos outros que não foram citados aqui, muito obrigado pela força.Ao amigo agrônomo e remador Felipe pelas conversas e por ter a disposição em remar cedo da manhã e fazer as nossas vidas muito mais saudáveis.A turma toda do LEM, Maú, Jorge, Eduardo, Luciana, Giselle, Ramada, Guil, Thiago, Bia e todos outros que fazem do laboratório um ambiente agradável de se estar.À equipe do Laboratório de Toxinologia da UnB, professora Márcia, que cedeu a estrutura para a realização dos testes, à Karlinha auxiliando no planejamento e execução dessa parte do trabalho e Jacqueline pelo apoio com os animais.Às meninas do LGM como Tati, Fernanda, Sinara, Michelle por auxiliar em algumas tarefas e estarem sempre dispostas a ajudar.Ao Alan por auxiliar em parte da pesquisa e ceder a estrutura do laboratório para desenvolver parte do meu trabalho Ao Pierre por fornecer as dicas tão valiosas e pela constante dedicação e disponibilidade em sanar dúvidas e ajudar.Aos membros da banca examinadora Dr. Luiz, Dr. Spartaco, Dr. Isaac, Dr. Marcelo e Dra. Maura pela disponibilidade em participar da banca.Ao meu orientador Carlos Bloch por todo o processo! Primeiramente por me instigar a fazer o doutorado, o que quase tinha desistido de fazer. Sempre mostrou que fazer ciência dá trabalho mas não é um martírio, mas sim uma diver...

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Different Molecular Mechanisms Account for Drought Tolerance in Coffea canephora var. Conilon

Cited by 26 publications

References 40 publications

Galactinol synthase transcriptional profile in two genotypes of Coffea canephora with contrasting tolerance to drought

Galactinol synthase transcriptional profile in two genotypes of Coffea canephora with contrasting tolerance to drought

Temperature Impacts the Response of Coffea canephora to Decreasing Soil Water Availability

Identificação e caracterização de peptídeos opioides presentes na fração proteica dos grãos de Coffea arabica

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