Different Molecular Capacity in the Induction of Apoptosis by Polychlorinated Biphenyl Congeners in Rat Renal Tubular Cell Cultures

Pérez-Reyes, Pedro L.; Sánchez-Alonso, Jesús Adolfo; López-Aparicio, Pilar; Recio, M.N.; Pérez-Albarsanz, M.A.

doi:10.1023/a:1015532707395

Bioscience Reports

2001

DOI: 10.1023/a:1015532707395

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Different Molecular Capacity in the Induction of Apoptosis by Polychlorinated Biphenyl Congeners in Rat Renal Tubular Cell Cultures

Pedro L. Pérez-Reyes

Jesús Adolfo Sánchez-Alonso

Pilar López-Aparicio

et al.

Abstract: The effects of a commercial polychlorinated biphenyl (PCB) mixture (Aroclor 1248) and two individual PCB congeners were evaluated on rat renal proximal tubule culture cell viability and internucleosomal DNA fragmentation (DNA ladder) characteristic of apoptosis. Treatment with Aroclor 1248 caused the loss of cell viability and promoted apoptosis in a concentration- and time-dependent manner. The two PCB congeners assessed can also induce apoptosis. However, the extent of apoptosis generated was greater for the… Show more

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Cited by 10 publications

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Morphological alterations of Vero cell exposed to coplanar PCB 126 and noncoplanar PCB 153

Shen

Chen

et al. 2011

Environmental Toxicology

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Polychlorinated biphenyls (PCBs) are widespread, persistent environmental contaminants that display a complex spectrum of toxicological properties. Exposure to PCBs has been associated with morphological anomalies in cell cultures. However, most mechanistic studies of PCBs' toxic activity have been focused on coplanar congeners. It is of importance to determine whether PCB treatment would influence cell configuration and whether these changes would depend on the structural characteristics of PCBs. In this study, we investigated cell morphological alteration in Vero cell cultures after exposure to coplanar PCB 126 and noncoplanar PCB 153. The survival of Vero cells was measured through the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test. Cytotoxicity results suggested that PCB congeners had a toxic, antiproliferative effect on Vero cells. Morphological studies described structural modifications and provided evidence that apoptosis might be the main cell death pathway in PCB 153-treated cells. The comparison between PCB 126 and PCB 153 indicated that the cell death mechanisms involved in coplanar or noncoplanar PCB congener exposure were different in Vero cells.

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Morphological alterations of Vero cell exposed to coplanar PCB 126 and noncoplanar PCB 153

Shen

Chen

et al. 2011

Environmental Toxicology

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Polychlorinated biphenyls (PCB-153) and (PCB-77) absorption in human liver (HepG2) and kidney (HK2) cells in vitro: PCB levels and cell death

Ghosh

Chen

et al. 2010

Environment International

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An understanding of congener specific cellular absorption of PCBs is important to the study of the organ specific body burden of an individual and to their toxic effects. We have previously demonstrated that single PCB congeners induce cytotoxicity, as evidenced by decreased cellular viability and accelerated apoptotic death. There is very little, if any, information available on the differences in toxicity due to the nature of absorption of PCBs in different cells. To obtain such information human liver (HepG2) cells (in medium with 10% FBS) were exposed to 70μM of both PCB-153 (non-coplanar hexachlorobiphenyl) and PCB-77 (coplanar tetrachlorobiphenyl), and human kidney (HK2) cells in serum free medium were exposed to 80 and 40 μM of PCB-153 and PCB-77 respectively, according to their LC50 values in these cells. Medium and cells were collected separately at each time interval from 30 minutes to 48 hours, and PCB concentrations were analyzed in both by GC-MS using biphenyl as an internal standard following hexane: acetone (50:50) extraction. We also performed trypan blue exclusion, DNA fragmentation and fluorescence microscopic studies in assessing cell viability and apoptotic cell death. About 40% of PCB-153 (35 μM, 50% of the maximum value) was detected in HepG2 cells within 30 minutes, and it reached its highest concentration at 6 hours (60 μM), concomitant with the PCB depletion in the medium (5μM). For PCB-77, the highest concentrations within the cells were reached at 3 hours. However, the absorption levels of PCB-153 and PCB-77 in HK2 cells reached their peaks at 3 and 6 hours respectively. Exposure of human liver and kidney cells to PCB-153 and PCB-77 caused accelerated apoptotic cell death in a time-dependent manner. The studies demonstrated that (1) liver cells initiate the absorption of PCBs much faster than kidney cells; however, the concentration reaches its maximum level much earlier in kidney cells; (2) both PCB-153 and PCB-77 induced enhanced apoptotic death in liver and kidney cells; (3) kidney cells are more vulnerable to PCBs based on the results of apoptosis and cellular viability, even with almost similar absorption or tissue burden of PCBs.

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Comparison of structure-dependent hormetic cytotoxicity induced by coplanar and non-coplanar PCB congeners

Chen

Shen

et al. 2010

Journal of Hazardous Materials

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Different Molecular Capacity in the Induction of Apoptosis by Polychlorinated Biphenyl Congeners in Rat Renal Tubular Cell Cultures

Cited by 10 publications

References 28 publications

Morphological alterations of Vero cell exposed to coplanar PCB 126 and noncoplanar PCB 153

Morphological alterations of Vero cell exposed to coplanar PCB 126 and noncoplanar PCB 153

Polychlorinated biphenyls (PCB-153) and (PCB-77) absorption in human liver (HepG2) and kidney (HK2) cells in vitro: PCB levels and cell death

Comparison of structure-dependent hormetic cytotoxicity induced by coplanar and non-coplanar PCB congeners

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