Different latent class models were used and evaluated for assessing the accuracy of campylobacter diagnostic tests: overcoming imperfect reference standards?

Asselineau, Julien; Paye, A; Bessède, Emilie; Perez, Paul; Proust‐Lima, Cécile

doi:10.1017/s0950268818001723

Cited by 9 publications

(5 citation statements)

References 41 publications

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“…culture, only double-positive cases by two independent PCRs could be considered as true Campylobacter sp. positives in case of negative culture [2,8,9]. We did not find a perfect correlation between the Campylobacter PCR results obtained on BD MAX TM and the two independent PCR formats.…”

Section: Discussioncontrasting

confidence: 64%

How to Interpret a Positive Campylobacter PCR Result Using the BD MAXTM System in the Absence of Positive Culture?

Gueudet

Paolini²,

Buissonnière

et al. 2019

JCM

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The aim of this study was to evaluate, using two independent polymerase chain reaction (PCR) formats, the results of Campylobacter detection by the BD MAXTM Enteric Bacterial Panel PCR (Becton Dickinson, Le Pont de Claix, France) in the absence of positive culture. A total of 77 samples found positive for Campylobacter on BD MAXTM but negative by culture were studied. Upon reception, one in-house real-time-PCR for Campylobacter sp. and a PCR with the RIDAGENE Bacterial Stool Panel (r-biopharm, Darmstadt, Germany) were performed. The data obtained using these two PCR formats were evaluated with respect to the cycle threshold (Ct) and fluorescence intensity values (FI) obtained on BD MAXTM. Ct and FI values were also obtained for 80 positive Campylobacter cases by culture. Among the 77 samples, 33 were positive with the two PCRs, and 37 remained negative. For the 33 double-positive PCRs samples, the Ct values obtained on BD MAXTM were lower than 30 in 93.9%, and FI > 2000 for 97% of cases. For the 37 double-negative PCRs samples, the Ct values obtained on BD MAXTM were <30 in only 18.9%, however FI were >2000 for 40.5% of cases. Positive culture cases had Ct values < 30 in 96.2% and FI > 2000 in 98.8%. We showed that the Ct values obtained on BD MAXTM can help to interpret the results. Almost 96% of the Campylobacter sp. cases detected by culture or with the two reference PCRs positive showed a Ct value on BD MAXTM, meaning that stools detected as positive with BD MAXTM and having a Ct > 30 may be false positives.

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Section: Discussioncontrasting

confidence: 64%

How to Interpret a Positive Campylobacter PCR Result Using the BD MAXTM System in the Absence of Positive Culture?

Gueudet

Paolini²,

Buissonnière

et al. 2019

JCM

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“…We did not perform the molecular method when culture was positive because of the excellent specificity with this latter method when MALDI-TOF gives a score up to 2 for the Campylobacter identified [ 14 ]. The diagnostic accuracy of the real-time PCR and the ELISA was previously assessed by Asselineau et al In their study, a latent class model confirmed that these CIDTs were valid to evaluate a test in the absence of a correct gold standard [ 16 ]. Furthermore, despite the fact that the real-time PCR and ELISA chosen are not perfect gold standards, they are based on two different principles and are known to be reliable and reproducible to detect C. jejuni and C. coli in stool samples [ 8 , 17 ].…”

Section: Discussionmentioning

confidence: 74%

Evaluation of CAMPYLOBACTER QUIK CHEK™ rapid membrane enzyme immunoassay to detect Campylobacter spp. antigen in stool samples

et al. 2021

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Campylobacter spp. enteritis is the most frequent bacterial enteritis in both adults and children and is sometimes a source of severe complications. Its diagnosis by culture suffers from a lack of sensitivity and delays the result, preventing an early initiation of optimal antibiotic therapy in some cases. Our aim was to test a new rapid immuno-enzymatic method for Campylobacter spp. diagnosis in comparison to a composite reference standard (CRS). Stool samples from the French National Reference Center for Campylobacter and Helicobacter were tested with the CAMPYLOBACTER QUIK CHEK™ (Abbott). The CRS used to consider a case positive for Campylobacter spp. was a positive culture and, in case of a negative culture, a positive result obtained with both an ELISA and a molecular test. One hundred and eight stools were included: 53 were positive according to the CRS. If performed alone, culture would have missed 5 cases which the CAMPYLOBACTER QUIK CHEK™ detected. Finally, the CAMPYLOBACTER QUIK CHEK™ showed a sensitivity of 96.2% and a specificity of 94.5% and is relevant for clinical practice. Given the characteristics of the new method, it can be used as a screening method for Campylobacter spp. detection.

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“…These are complex methods that rely on the assumption of conditional independence between diagnostic tests and have the potential to correctly estimate the diagnostic accuracy of these molecular tests. These methods define the true target condition status as two latent classes and estimate the sensitivity and specificity of diagnostic tests based on these latent classes [51][52][53].…”

Section: Discussionmentioning

confidence: 99%

Comparison of three PCR‐based methods to detect Loa loa and Mansonella perstans in long‐term frozen storage dried blood spots

Ta‐Tang

Febrer‐Sendra

Berzosa

et al. 2022

Tropical Med Int Health

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Objectives Loa loa and Mansonella perstans are two very common filarial species in Africa. Although microscopy is the traditional diagnostic method for human filariasis, several polymerase chain reaction (PCR) methods have emerged as an alternative approach for identifying filarial parasites. The aim of this study is to compare three molecular methods and decide which is the most suitable for diagnosing human loiasis and mansonellosis in non‐endemic regions using dried blood spot (DBS) as a medium for sample collection and storage. Methods A total of 100 DBS samples, with their corresponding thin and thick blood smears, were selected for this study. Microscopy was used as the reference method to diagnose and calculate the microfilaraemia. Filarial DNA was extracted using the saponin/Chelex method and the DNA isolated was assayed by Filaria‐real time‐PCR, filaria‐nested PCR, and cytochrome oxidase I PCR. All PCR products were subsequently purified and sequenced. The statistical values for each molecular test were calculated and compared. Results Overall, 64 samples were identified as negative by all tests and a further 36 samples were positive by at least one of the methods used. The sensitivity and specificity were similar for the different molecular methods, all of which demonstrated good agreement with microscopy. Conclusions Based on this study, and from a practical point of view (single and short amplification round), the optimal technique for diagnosing filarial infection in non‐endemic regions is filaria‐real time‐PCR, which presents high sensitivity and specificity and is also able to detect a wide range of human filariae.

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Different latent class models were used and evaluated for assessing the accuracy of campylobacter diagnostic tests: overcoming imperfect reference standards?

Cited by 9 publications

References 41 publications

How to Interpret a Positive Campylobacter PCR Result Using the BD MAXTM System in the Absence of Positive Culture?

How to Interpret a Positive Campylobacter PCR Result Using the BD MAXTM System in the Absence of Positive Culture?

Evaluation of CAMPYLOBACTER QUIK CHEK™ rapid membrane enzyme immunoassay to detect Campylobacter spp. antigen in stool samples

Comparison of three PCR‐based methods to detect Loa loa and Mansonella perstans in long‐term frozen storage dried blood spots

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