Different kinetics of the regulation of respiration in permeabilized cardiomyocytes and in HL-1 cardiac cells

Anmann, Tiia; Guzun, Rita; Béraud, Nathalie; Pelloux, Sophie; Kuznetsov, Andrey V.; Kogerman, Lembi; Käämbre, Tuuli; Sikk, Peeter; Paju, Kalju; Peet, Nadja; Seppet, Enn; Ojeda, Carlos; Tourneur, Yves; Saks, Valdur

doi:10.1016/j.bbabio.2006.09.008

Cited by 66 publications

(61 citation statements)

References 27 publications

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“…This has been previously reported [166][167][168][169][170][171][172][173][174][175][176] and explained by local restrictions of ADP diffusion in the cells, including limitation of its diffusion across the mitochondrial outer membrane [33,177]. In permeabilized cardiomyocytes, treatment with trypsin resulted in dramatic changes in intracellular structure that were associated with a threefold decrease in apparent K m for ADP in the regulation of respiration [176,178]. In contrast to permeabilized cardiomyocytes, in NB HL-1 cells the apparent K m for exogenous ADP (25 G 4 mM) was 14 times lower Red as described in [178].…”

Section: Heterogeneity Of Adp Diffusion In Permeabilized Cells: Impormentioning

confidence: 78%

“…In permeabilized cardiomyocytes, treatment with trypsin resulted in dramatic changes in intracellular structure that were associated with a threefold decrease in apparent K m for ADP in the regulation of respiration [176,178]. In contrast to permeabilized cardiomyocytes, in NB HL-1 cells the apparent K m for exogenous ADP (25 G 4 mM) was 14 times lower Red as described in [178]. (C, D) Different kinetics of regulation of the respiration in permeabilized adult cardiomyocytes and NB HL-1 cells.…”

Section: Heterogeneity Of Adp Diffusion In Permeabilized Cells: Impormentioning

confidence: 99%

“…(C, D) Different kinetics of regulation of the respiration in permeabilized adult cardiomyocytes and NB HL-1 cells. (C) ADP kinetic protocols and representative respiration traces for permeabilized cardiomyocytes recorded using a two-channel, high-resolution respirometer (Oroboros Oxygraph-2k, Oroboros, Innsbruck, Austria) in respirometry medium Mitomed [178]. Mitochondrial respiration increases with stepwise increasing concentrations of ADP in the range of 0.05 to 2 mM for cardiomyocytes to reach the saturated rate of respiration (V max ).…”

Section: Heterogeneity Of Adp Diffusion In Permeabilized Cells: Impormentioning

confidence: 99%

“…Atractyloside (Atr, 30 mM) results in a decrease in respiration back to V o due to inhibition of adeninedinucleotide translocase. (Reproduced from [178] with permission). (D) Comparison of respiration kinetics as normalized respiration rates versus [ADP] in permeabilized adult cardiomyocytes and in permeabilized HL-1 NB cells determined in experiments described in (C).…”

Section: Heterogeneity Of Adp Diffusion In Permeabilized Cells: Impormentioning

confidence: 99%

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Integrated and Organized Cellular Energetic Systems: Theories of Cell Energetics, Compartmentation, and Metabolic Channeling

Saks

Monge

Anmann

et al. 2007

Molecular System Bioenergetics

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Bioenergetics as a part of biophysical chemistry and biophysics is a quantitative science that is based on several fundamental theories. The definition of energy itself is given by the first law of thermodynamics, and application of the second law of thermodynamics shows that living cells can function as open systems only where the internal order (low entropy state) is maintained due to increasing the entropy in the surrounding medium (Schrödinger's principle of negentropy). For this, the exchange of mass is needed, which gives rise to metabolism as the sum of catabolism and anabolism, and the free energy changes during catabolic reactions supply energy for all cellular work -osmotic, mechanical, and biochemical. The free energy changes during metabolic reactions obey the rules of chemical thermodynamics, which deals with Gibbs free energy of chemical reactions and with electrochemical potentials. For application of these theories to the integrated systems in vivo, complex cellular organization should be accounted for: macromolecular crowding; metabolic channeling and functional coupling mechanisms due to close and tight protein-protein interactions; compartmentation of the enzymes due to their attachment to the subcellular membranes or connection to the cytoskeleton; and both macro-and microcompartmentation of substrates and metabolites in the cells. Because of this, almost all processes important for cell life are localized within small areas of the cell, and for their integration effective systems of communication, including compartmentalized energy transfer systems, are required. These are represented in muscle cells by the phosphotransfer networks, mostly by the creatine kinase and adenylate kinase systems, whose alterations under ischemic conditions contribute significantly to acute ischemic contractile failure of the heart. 59 Molecular System Bioenergetics: Energy for Life. Edited by Valdur Saks

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Section: Heterogeneity Of Adp Diffusion In Permeabilized Cells: Impormentioning

confidence: 78%

Section: Heterogeneity Of Adp Diffusion In Permeabilized Cells: Impormentioning

confidence: 99%

Section: Heterogeneity Of Adp Diffusion In Permeabilized Cells: Impormentioning

confidence: 99%

Section: Heterogeneity Of Adp Diffusion In Permeabilized Cells: Impormentioning

confidence: 99%

See 2 more Smart Citations

Integrated and Organized Cellular Energetic Systems: Theories of Cell Energetics, Compartmentation, and Metabolic Channeling

Saks

Monge

Anmann

et al. 2007

Molecular System Bioenergetics

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“…These early localized alterations to the functional output of the mitochondrion may reflect either an indirect 'structural' alteration to the adjacent sarcomere, or a more direct 'metabolic' shift in the mitochondria, as defined by the subcellular localization within the cardiomyocyte. This 'structural shift' has been shown in isolated preparations of cardiomyocytes and myocardial fibrils, where Ca 21 and ATP treatment resulted in strong contraction and concurrent spatial and functional alterations to mitochondria [217]. Alterations to ADP stimulated respiration were observed in interfibrillar populations of mitochondria from animal models of cardiomyopathy [70].…”

Section: Mitochondrial Morphology and Structurementioning

confidence: 99%

Mitochondria: A mirror into cellular dysfunction in heart disease

White

Edwards

Cordwell

et al. 2008

Proteomics Clinical Apps

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Cardiovascular (CV) disease is the single most significant cause of morbidity and mortality worldwide. The emerging global impact of CV disease means that the goals of early diagnosis and a wider range of treatment options are now increasingly pertinent. As such, there is a greater need to understand the molecular mechanisms involved and potential targets for intervention. Mitochondrial function is important for physiological maintenance of the cell, and when this function is altered, the cell can begin to suffer. Given the broad range and significant impacts of the cellular processes regulated by the mitochondria, it becomes important to understand the roles of the proteins associated with this organelle. Proteomic investigations of the mitochondria are hampered by the intrinsic properties of the organelle, including hydrophobic mitochondrial membranes; high proportion of basic proteins (pI greater than 8.0); and the relative dynamic range issues of the mitochondria. For these reasons, many proteomic studies investigate the mitochondria as a discrete subproteome. Once this has been achieved, the alterations that result in functional changes with CV disease can be observed. Those alterations that lead to changes in mitochondrial function, signaling and morphology, which have significant implications for the cardiomyocyte in the development of CV disease, are discussed.

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Integrated and Organized Cellular Energetic Systems

Saks

Monge

Guzun

et al. 2009

Wiley Encyclopedia of Chemical Biology

View full text Add to dashboard Cite

Bioenergetics as a part of biophysical chemistry and biophysics is a quantitative science that is based on several fundamental theories. The definition of energy itself is given by the first law of thermodynamics, and application of the second law of thermodynamics shows that the living cells can only function as open systems in which internal order (low entropy state) is maintained by increasing the entropy in surrounding medium (Schrödinger's principle of negentropy). For this mechanism, exchange of mass is needed that yields metabolism as the sum of catabolism and anabolism. The free‐energy changes taking place during metabolic reactions obey rules of chemical thermodynamics, which deals with Gibbs free energy of chemical reactions and with electrochemical potentials. For application of these theories to the integrated systems in vivo , complex cellular organization should be accounted for: macromolecular crowding, metabolic channeling and functional coupling mechanisms caused by close and tight protein–protein interactions, compartmentation of enzymes, as well as both macrocompartmentation and microcompartmentation of substrates and metabolites in cells should be considered. For integration of these system components, effective methods of communication, including compartmentalized energy transfer systems, are required. These systems are represented in muscle cells by phosphotransfer networks, mostly by creatine kinase and adenylate kinase systems. System analysis of these networks shows their central role both in energy transfer and metabolic feedback regulation of mitochondrial function, in regulation of ion fluxes and cellular energetics during increased workload by synchronizing the cellular electrical and mechanical activities with energy supply and substrate oxidation.

show abstract

Different kinetics of the regulation of respiration in permeabilized cardiomyocytes and in HL-1 cardiac cells

Cited by 66 publications

References 27 publications

Integrated and Organized Cellular Energetic Systems: Theories of Cell Energetics, Compartmentation, and Metabolic Channeling

Integrated and Organized Cellular Energetic Systems: Theories of Cell Energetics, Compartmentation, and Metabolic Channeling

Mitochondria: A mirror into cellular dysfunction in heart disease

Integrated and Organized Cellular Energetic Systems

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