Different Immunology Mechanisms of Phellinus igniarius in Inhibiting Growth of Liver Cancer and Melanoma Cells

Zhou, Chuanpeng; Jiang, Songsong; Wang, Cuiyan; Li, Rong; Che, Huilian

doi:10.7314/apjcp.2014.15.8.3659

Cited by 12 publications

(7 citation statements)

References 16 publications

(11 reference statements)

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“…The immunity-modulating, anti-inflammatory, and antitumor effects of Phellinus spp. are well documented (Zhou et al, 2014;Suabjakyong et al, 2015;Gao et al, 2017;Wang et al, 2017). In our previous study, the extract of Phellinus ameliorated intestinal injury and improved the body weight of mice with colitis (Sun et al, 2018).…”

Section: Introductionmentioning

confidence: 86%

Impact of Phellinus gilvus mycelia on growth, immunity and fecal microbiota in weaned piglets

Sun

Deng

et al. 2020

PeerJ

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Background Antibiotics are the most commonly used growth-promoting additives in pig feed especially for weaned piglets. But in recent years their use has been restricted because of bacterial resistance. Phellinus, a genus of medicinal fungi, is widely used in Asia to treat gastroenteric dysfunction, hemrrhage, and tumors. Phellinus is reported to improve body weight on mice with colitis. Therefore, we hypothesize that it could benefit the health and growth of piglets, and could be used as an alternative to antibiotic. Here, the effect of Phellinus gilvus mycelia (SH) and antibiotic growth promoter (ATB) were investigated on weaned piglets. Methods A total of 72 crossbred piglets were randomly assigned to three dietary treatment groups (n = 4 pens per treatment group with six piglets per pen). The control group was fed basal diet; the SH treatment group was fed basal diet containing 5 g/kg SH; the ATB treatment group was feed basal diet containing 75 mg/kg aureomycin and 20 mg/kg kitasamycin. The experiment period was 28 days. Average daily gain (ADG), average daily feed intake (ADFI), and feed intake to gain ratio were calculated. The concentrations of immunoglobulin G (IgG), interleukin-1β (IL-1β), tumor necrosis factor (TNF)-α and myeloperoxidase (MPO) in serum were assessed. Viable plate counts of Escherichia coli in feces were measured. Fecal microbiota was analyzed via the 16S rRNA gene sequencing method. Results The ADG (1–28 day) of piglets was significantly higher in SH and ATB treatment groups (P < 0.05) compared to the control, and the ADG did not show significant difference between SH and ATB treatment groups (P > 0.05). Both SH and ATB treatments increased the MPO, IL-1β, and TNF-α levels in serum compared to the control (P < 0.05), but the levels in SH group were all significantly higher than in the ATB group (P < 0.05). Fecal microbiological analysis showed that viable E. coli counts were dramatically decreased by SH and ATB. The 16S rRNA gene sequencing analysis showed that ATB shifted the microbiota structure drastically, and significantly increased the relative abundance of Prevotella, Megasphaera, and Faecalibacterium genera. But SH slightly influenced the microbiota structure, and only increased the relative abundance of Alloprevotella genus. Conclusion Our work demonstrated that though SH slightly influenced the microbiota structure, it markedly reduced the fecal E. coli population, and improved growth and innate immunity in piglets. Our finding suggested that SH could be an alternative to ATB in piglet feed.

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Section: Introductionmentioning

confidence: 86%

Impact of Phellinus gilvus mycelia on growth, immunity and fecal microbiota in weaned piglets

Sun

Deng

et al. 2020

PeerJ

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“…P. igniarius possesses high anti-inflammatory, antioxidant and antitumor biological activities due to its accumulation of various secondary metabolites, including polysaccharides, flavonoids, polyphenols, steroids and organic acids (Dong et al, 2015; Shou et al, 2016). Notably, recent studies have creatively focused on the potential functions of P. igniarius extracts and their constituent compounds in the prevention and treatment of cancer (Zhou et al, 2014; Sangdee et al, 2017). A considerable amount of evidence indicates that P. igniarius extracts resulting from water, alcohol, ethyl acetate and other solvent extractions have a significant inhibitory effect on various tumor cells, such as S180, PC3, SK-HEP-1, and HT-29 cells (Yang et al, 2006; Song et al, 2008; Jeon et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

A Combined Phytochemistry and Network Pharmacology Approach to Reveal the Potential Antitumor Effective Substances and Mechanism of Phellinus igniarius

Dong¹,

Qiu

Zhu

et al. 2019

Front. Pharmacol.

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Phellinus igniarius (P. igniarius) is a medicinal fungus that is widely used in East Asia for the adjuvant treatment of cancer. To elucidate the antitumor effective substances and mechanism of P. igniarius, we designed an approach incorporating cytotoxicity screening, phytochemical analysis, network pharmacology construction, and cellular and molecular experiments. The dichloromethane extract of P. igniarius (DCMPI) was identified as the active portion in HT-29 cells. Nineteen constituents were identified, and 5 were quantified by UPLC-ESI-Q/TOF-MS. Eight ingredients were obtained in the network pharmacology study. In total, 473 putative targets associated with DCMPI and 350 putative targets related to colon cancer were derived from online databases and target prediction tools. Protein-protein interaction networks of drug and disease putative targets were constructed, and 84 candidate targets were identified based on topological features. Pathway enrichment analysis showed that the candidate targets were mostly related to reactive oxygen species (ROS) metabolic processes and intrinsic apoptotic pathways. Then, a cellular experiment was used to validate the drug-target mechanisms predicted by the system pharmacology analysis. Experimental results showed that DCMPI increased intracellular ROS levels and induced HT-29 cell apoptosis. Molecular biology experiments indicated that DCMPI not only increased Bax and Bad protein expression and promoted PARP and caspase-3/9 cleavage but also down-regulated Bcl-2 and Bcl-xl protein levels to induce apoptosis in HT-29 cells. In conclusion, our study provides knowledge on the chemical composition and antitumor mechanism of P. igniarius, which may be exploited as a promising therapeutic option for colon cancer.

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“…IL-2 binds to the IL-2R on T-lymphocytes in an autocrine and paracrine manner. As one of the most important regulating factors of immune system, IL-2 can promote the proliferation and differentiation of T cells and B cells, activate the tumor killer cells (LAK, TIL) and improve production of TNF (Brady et al, 2010;Cui et al, 2014). Grimm (Grimm et al, 1982) In the present study, we assessed the frequency of LAK cells (LAK-NK, LAK-NKTand LAK-T cells) cultured from the peripheral blood of healthy volunteers and their ability to kill tumor cells based on IL-2 and IL-2R SNP genotypes.…”

Section: ) Impact Of Il-2 and Il-2r Snps On Tumor-killing Activitymentioning

confidence: 99%

Impact of IL-2 and IL-2R SNPs on Proliferation and Tumor-killing Activity of Lymphokine-Activated Killer Cells from Healthy Chinese Blood Donors

Meng²,

Tian³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Asian Pac J Cancer Prev, 15 (18), 7965-7970 IntroductionBiologically based immunotherapies are a new method for treating tumors using biological products in vivo to augment the tumor specific immune response of patients, maintain their physiological balance, or protect against tumors. Alternatively, in vitro methods can be used to expand or activate the cytotoxic effector cells that regulate the in vivo biological response. The goal of such in vitro methods is to enhance the anti-tumor activity of effector cells before infusing them back into patients where they kill the tumor. Erhan's studies found that most cancers occur not to transferred malign cells out of the body, but by a malign transformation of self-cells (Erhan et al., 2013). But the effects of whole cell immunization for prevention of human cancers is not clear. New studies are needed to evaluate whole cell type immunization on cancer prevention. Lymphokine-activated killer (LAK) cells are widely used in immunotherapy. LAK cells, first described in the 1980s, are activated natural killer (NK)

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Different Immunology Mechanisms of Phellinus igniarius in Inhibiting Growth of Liver Cancer and Melanoma Cells

Cited by 12 publications

References 16 publications

Impact of Phellinus gilvus mycelia on growth, immunity and fecal microbiota in weaned piglets

Impact of Phellinus gilvus mycelia on growth, immunity and fecal microbiota in weaned piglets

A Combined Phytochemistry and Network Pharmacology Approach to Reveal the Potential Antitumor Effective Substances and Mechanism of Phellinus igniarius

Impact of IL-2 and IL-2R SNPs on Proliferation and Tumor-killing Activity of Lymphokine-Activated Killer Cells from Healthy Chinese Blood Donors

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