Different immune functions of peripheral blood, regional lymph node, and tumor infiltrating lymphocytes in lung cancer patients

Abstract

Immune functions of peripheral blood (PBL), regional lymph node (RLNL), and tumor infiltrating lymphocytes (TIL) were evaluated in lung cancer patients. PBL had many natural killer (NK) cells and the highest NK activity, and it showed the highest augmentation of NK activity by interferon-gamma (IFN-gamma) + recombinant interleukin-2 (rIL-2) among the three groups of lymphocytes. PBL had high lymphokine-activated killer (LAK) activity of against a broad spectrum of cell lines and moderate activity against autologous tumor cells by increased effector to target (ET) ratio but the lowest ability of IL-2 production of the three groups of lymphocytes. The RLNL not associated with tumor metastasis had a few NK cells and lower NK activity than PBL, but its LAK activity was almost the same but not greater than that of PBL. RLNL had the highest ability of IL-2 production among the three groups of lymphocytes. All activities of RLNL associated with tumor metastasis were lower than those not associated with tumor metastasis. TIL exclusively consisted of T-cells, especially cytotoxic/suppressor T-lymphocytes. NK activity and lymphocyte blastogenesis of TIL were lower than those of other groups. The LAK activity of TIL differed greatly with the case, and it was the highest against autologous tumor cells among the three groups of lymphocytes in three of eight cases. These findings showed that PBL, RLNL, and TIL had characteristic subpopulations of lymphocytes and different functions of host immune responses in lung cancer. Efficient augmentation of the characteristic immune responses will lead to a more effective total cancer therapy.

IL‐8 induces exocytosis of arginase 1 by neutrophil polymorphonuclears in nonsmall cell lung cancer

“…13,14 To study the potential role of ARG1 and of PMNs, we first performed immunohistochemistry for ARG1 on tumor tis-sue sections from 30 cases of NSCLC. In general the extent of intratumoral infiltration by PMN was variable among the different cases, but in the majority of them tumor-infiltrating PMNs displayed a faint reactivity for ARG1 or were negative (Fig.…”

“…Indeed, in NSCLC tumor-infiltrating lymphocytes (TILs) are nonproliferating, anergic cells, 13,14 although the underlying immune suppressive mechanism(s) have not been clearly identified.…”

Pulmonary perspective: immunology in diagnosis and treatment of lung cancer

“…Thus, both in vitro and in vivo immune responses have been found to be reduced in cancer patients [6]. The immunosuppressive response includes: a decrease of delayed type hypersensitivity reactions; abnormal distribution of T-lymphocyte subsets; reduced cytolytic activity of peripheral blood mononuclear cells; and impaired proliferative response of lymphocytes to various mitogens [7][8][9][10]. More recently, defects in cytokine production have also been reported in cancer patients [6,10].…”

Human stomach carcinoma-specific T cells derived from the tumour-draining lymph nodes

“…This is a good model of cellular reaction to tumour-associated immunogenic structures (Anchini et al, 1987(Anchini et al, , 1989Muul et al, 1987;Topalian et al, 1989) and one in which immunotherapies have proved effective (Rosenberg et al, 1986). Only a few other spontaneous tumours have been analysed, and only a few reports (Nakamura et al, 1988;Mukhopadhyaya et al, 1989;Tatake et al, 1989) have compared immune responses between lymphocytes isolated from peripheral blood, regional lymph nodes and tumours in different malignant diseases. Most deal with lymphokine-activated killer (LAK) cells (Itoh et al, 1986;Rabinowich et al, 1987) and cytotoxic T lymphocytes (CTLs) originating from melanomas (Muul et al, 1987;Anchini et al, 1989;Topalian et al, 1989).…”