Different genotypes of Yersinia enterocolitica 4/O:3 strains widely distributed in butcher shops in the Munich area

Fredriksson-Ahomaa, Maria; Koch, Ulrike; Klemm, Carolin; Bucher, Michael; Stolle, A.

doi:10.1016/j.ijfoodmicro.2004.02.016

Cited by 42 publications

(24 citation statements)

References 7 publications

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“…The molecular methods most frequently used include restriction analysis of both plasmids (Nesbakken et al, 1987;Kapperund et al, 1990) and chromosomes (Blumberg et al, 1991), randomly amplified polymorphic DNA analysis (Rasmussen et al, 1994), ribotyping (Andersen & Saunders, 1990;Lobato et al, 1998) and PFGE (Najdenski et al, 1994;Saken et al, 1994;Iteman et al, 1996;Fredriksson-Ahomaa et al, 2004). The enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) technique has not been used extensively, and its real potential as a tool for typing and in epidemiological studies of Y. enterocolitica has yet to be recognized (Sachdeva & Virdi, 2004;Wojciech et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Molecular typing and virulence markers of Yersinia enterocolitica strains from human, animal and food origins isolated between 1968 and 2000 in Brazil

Falcão

Pitondo-Silva³

et al. 2006

Journal of Medical Microbiology

102

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Molecular typing and virulence markers were used to evaluate the genetic profiles and virulence potential of 106 Yersinia enterocolitica strains. Of these strains, 71 were bio-serotype 4/O : 3, isolated from human and animal clinical material, and 35 were of biotype 1A or 2 and of diverse serotypes, isolated from food in Brazil between 1968 and 2000. Drug resistance was also investigated. All the strains were resistant to three or more drugs. The isolates showed a virulence-related phenotype in the aesculin, pyrazinamidase and salicin tests, except for the food isolates, only two of which were positive for these tests. For the other phenotypic virulence determinants (autoagglutination, Ca ++ dependence and Congo red absorption), the strains showed a diverse behaviour. The inv, ail and ystA genes were detected in all human and animal strains, while all the food isolates were positive for inv, and 3 % of them positive for ail and ystA. The presence of virF was variable in the three groups of strains. The strains were better discriminated by PFGE than by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). A higher genomic similarity was observed among the 4/O : 3 strains, isolated from human and animal isolates, than among the food strains, with the exception of two food strains possessing the virulence genes and grouped close to the 4/O : 3 strains by ERIC-PCR. Unusually, the results revealed the virulence potential of a bio-serotype 1A/O : 10 strain, suggesting that food contaminated with Y. enterocolitica biotype 1A may cause infection. This also suggests that ERIC-PCR may be used as a tool to reveal clues about the virulence potential of Y. enterocolitica strains. Furthermore, the results also support the hypothesis that animals may act as reservoirs of Y. enterocolitica for human infections in Brazil, an epidemiological aspect that has not been investigated in this country, confirming data from other parts of the world. INTRODUCTIONYersinia enterocolitica is the most prevalent Yersinia species connected to disease in humans (Bottone, 1999; RobinsBrowne, 2001). It has also frequently been isolated from animals, food and the environment (Fredriksson-Ahomaa et al., 1999;Thoerner et al., 2003; Falcão et al., 2004).Y. enterocolitica is responsible for gastroenteritis and other syndromes in humans and animals (Bottone, 1999 biochemical and serological tests that enable the differentiation of the strains into diverse bio-serotypes (Aleksic & Bockemühl, 1999). Also, pathogenic strains are related to some phenotypic characteristics, such as pyrazinamidase production, aesculin hydrolysis, salicin fermentation, calcium dependence, autoagglutination and Congo red absorption (Bottone, 1999). Molecular genetic studies have emphasized the importance of a virulence plasmid (pYV) that encodes various virulence genes, among them virF, which is an important transcriptional regulator of other plasmid genes, as well as the role of chromosomal virulence genes that mediate cell invasion (inv and ail) and produce a therm...

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Section: Introductionmentioning

confidence: 99%

Molecular typing and virulence markers of Yersinia enterocolitica strains from human, animal and food origins isolated between 1968 and 2000 in Brazil

Falcão

Pitondo-Silva³

et al. 2006

Journal of Medical Microbiology

102

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“…This hypothesis is supported by the fact that the area where the dog was rescued was located between a large abattoir for pigs and a conventional market with a large number of butchers. 7 Several studies 4,8,11 have suggested that pork is associated with the transmission of Y. enterocolitica 4:O3 to dogs. In addition, in Korea, Y. enterocolitica O3 has predominantly been isolated from pigs and dogs.…”

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confidence: 99%

Hepatic Yersiniosis Caused by Yersinia Enterocolitica 4:O3 in an Adult Dog

et al. 2011

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Abstract.A 2-year-old Pekinese dog was diagnosed with hepatic yersiniosis. Grossly, white-to-yellow nodules consisting of degenerated inflammatory cells, cell debris, and bacterial clumps were scattered throughout the liver. Histopathologically, suppurative and necrotizing hepatitis was apparent. Yersinia enterocolitica biotype 4, serotype O3 (4:O3) was identified and confirmed in the liver immunohistochemically, using a monoclonal antibody. The virulence genes ystA and ail were detected, but the isolate was negative for autoagglutination and calcium-dependent growth. To confirm systemic yersiniosis in animals, it is imperative that the organism(s) be identified because the hepatic lesions are similar to those of Y. pseudotuberculosis and other diseases, including plague, which is also a zoonotic pathogen.

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“…In Japan, the Y. enterocolitica O:8 strains have been isolated from stream water [9, 65]. Distribution of genotypes of Y. enterocolitica 4/O:3 strains in butcher shops in Munich has been studied with PFGE using NotI, ApaI, and XhoI enzymes [66]. Twelve genotypes were obtained among 33 isolates from 14 pork and two environmental samples, demonstrating that several different strains were distributed in butcher shops.…”

Section: Contamination Of Food and Environmentmentioning

confidence: 99%

“…Pathogenic Y. enterocolitica can be transmitted from slaughterhouses to meat-processing plants and then to retail level via contaminated pig carcasses and offal [66, 70]. Contaminated pork and offal are important transmission vehicles from retail shops to humans [70].…”

Section: Possible Routes Of Transmissionmentioning

confidence: 99%

Behavior ofYersinia enterocoliticain Foods

Bari

Hossain

Isshiki

et al. 2011

Journal of Pathogens

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Yersinia enterocolitica are ubiquitous, being isolated frequently from soil, water, animals, and a variety of foods. They comprise a biochemically heterogeneous group that can survive and grow at refrigeration temperatures. The ability to propagate at refrigeration temperatures is of considerable significance in food hygiene. Virulent strains of Yersinia invade mammalian cells such as HeLa cells in tissue culture. Two chromosomal genes, inv and ail, were identified for cell invasion of mammalian. The pathogen can cause diarrhoea, appendicitis and post-infection arthritis may occur in a small proportion of cases. The most common transmission route of pathogenic Y. enterocolitica is thought to be fecal-oral via contaminated food. Direct person-to-person contact is rare. Occasionally, pathogenic Y. enterocolitica has been detected in vegetables and environmental water; thus, vegetables and untreated water are also potential sources of human yersiniosis. However, the isolation rates of pathogenic Y. enterocolitica have been low, which may be due to the limited sensitivity of the detection methods. To identify other possible transmission vehicles, different food items should be studied more extensively. Many factors related to the epidemiology of Y. enterocolitica, such as sources, transmission routes, and predominating genotypes remain obscure because of the low sensitivity of detection methods.

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Different genotypes of Yersinia enterocolitica 4/O:3 strains widely distributed in butcher shops in the Munich area

Cited by 42 publications

References 7 publications

Molecular typing and virulence markers of Yersinia enterocolitica strains from human, animal and food origins isolated between 1968 and 2000 in Brazil

Molecular typing and virulence markers of Yersinia enterocolitica strains from human, animal and food origins isolated between 1968 and 2000 in Brazil

Hepatic Yersiniosis Caused by Yersinia Enterocolitica 4:O3 in an Adult Dog

Behavior ofYersinia enterocoliticain Foods

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