Different Genetic Supports for the
            <i>tet</i>
            (S) Gene in Enterococci

Novais, Carla; Freitas, Ana R.; Silveira, Eduarda; Baquero, Fernando; Peixe, Luı́sa; Roberts, Adam P.; Coque, Teresa M.

doi:10.1128/aac.00758-12

Cited by 17 publications

(13 citation statements)

References 14 publications

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“…Accordingly, the present study recorded tet (M) at many sampling sites whereas tet (S), tet (A) and tet (B) were found in only limited sampling sites ( Figure 5). Further, a high detection frequency of tet (M) was recorded in this study and similar results were reported in Vietnamese shrimp and fish ponds as well (Hoa et al, 2011;Novais et al, 2012). Tetracycline contamination in aquaculture environment 14 The tet (M) gene has been detected at high percentages in bacteria which w aquaculture sites in Korea and Japan (Kim et al, 2004) Similarly, this study als in many isolated resistant bacteria strains (Table 3).…”

Section: Pcr Detection Of Args In Water Samplessupporting

confidence: 84%

Occurrence and distribution of tetracycline resistance determinants and their pollution profile in the aquaculture environment in Sri Lanka

Liyanage

Manage

2019

J. Natn. Sci. Foundation Sri Lanka

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Tetracycline (TET) has been extensively used in aquaculture for chemotherapy against various fish diseases. The contamination levels in 16 aquaculture farms in Sri Lanka were analysed using high performance liquid chromatography (HPLC). The presence of antibiotic-resistant bacteria was identified using the 16SrRNA gene sequencing and the corresponding antibiotic resistant genes (ARGs) were screened at each location using the PCR method. The concentration of TET in the aquaculture farm samples ranged between 0.001± 0.001 -0.112± 0.017 ppm. Bacillus and Staphylococcus were recorded as the most dominant resistant bacterial genera against TET. Acinetobacter sp., Achromabacter sp., Staphylococcus sp., Micrococcus sp. were also identified as resistant bacteria. In the present study, tet (M) and tet (A) were the abundant resistance genes (11/16 isolates), followed by tet (S) (5/16) and tet (B) (4/16). It was found that tet (M) is one of the most widely distributed tetracycline resistance determinants in the aquaculture environment in Sri Lanka.

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Section: Pcr Detection Of Args In Water Samplessupporting

confidence: 84%

Occurrence and distribution of tetracycline resistance determinants and their pollution profile in the aquaculture environment in Sri Lanka

Liyanage

Manage

2019

J. Natn. Sci. Foundation Sri Lanka

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“…The F type harboured IS Efm2 , representative of the IS 256 family. Thus far, in enterococci this IS has been solely observed inserted between orf13 and tetS within CTn 6000 transposon [43]. In our study, we report for the first time the insertion of IS Efm2 into Tn 1546 .…”

Section: Discussionmentioning

confidence: 50%

Diversity of plasmids and Tn1546-type transposons among VanA Enterococcus faecium in Poland

Wardal

Kuch

Gawryszewska

et al. 2016

Eur J Clin Microbiol Infect Dis

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The objective of this study was to investigate the antimicrobial resistance, Tn1546 transposon variability and plasmid diversity among Polish vancomycin-resistant Enterococcus faecium (VREfm) isolates of VanA phenotype in the context of their clonal structure. Two hundred sixteen clinical VREfm isolates collected between 1997 and 2010 were studied by antimicrobial susceptibility testing, MLST, MLVA and detection of IS16, esp Efm, pilA, intA and plasmid-specific genes by PCR. Tn1546 structure was revealed by overlapping PCR and sequencing. Selected isolates were subjected to PFGE-S1 and Southern hybridization analyses. The vast majority of the isolates (95.8 %) belonged to lineages 17/18 (during the whole study period 1997–2010) and 78 (mostly in 2006–2010) of hospital-adapted meroclone of E. faecium. All isolates displayed a multi-drug resistance phenotype. Twenty-eight Tn1546 types (including 26 novel ones) were associated with eight different ISs (IS1216, IS1251, ISEfa4, ISEfa5, ISEfm2, ISEf1, IS3-like, ISEfm1-like). The vanA-determinant was typically located on plasmids, which most commonly carried rep2pRE25, rep17pRUM, rep18pEF418, rep1pIP501, ω-ε-ζ and axe-txe genes. VanA isolates from 1997–2005 to 2006–2010 differed in clonal composition, prevalence of gentamicin- and tetracycline-resistance and plasmidome. Our analysis revealed high complexity of Tn1546-type transposons and vanA-plasmids, and suggested that diverse genetic events, such as conjugation transfer, recombination, chromosomal integration and DNA mutations shaped the structure of these elements among Polish VREfm.Electronic supplementary materialThe online version of this article (doi:10.1007/s10096-016-2804-8) contains supplementary material, which is available to authorized users.

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“…that carry multidrug-resistant determinants have been shown to be transferable to E.coli from A.salmonicida, A.hydrophila, V.cholerae, Shigella species, Salmonella species and E.coli (Suzuki et al 2008;Sorum 2006). Genes coding for tetracycline resistance in fish farm bacteria and human clinical isolates in Japan has shown high similarity, suggesting that they were derived from the same source (Novais et al 2012;Vincent et al 2015). Furthermore, in laboratory experiments, transfer of tetracycline resistance from marine strains of Photobacterium species, Vibrio species, Aeromonas species and Pseudomonas species into E.coli by conjugation was recorded suggesting that transfer of resistance gene from marine bacteria to bacteria associated with the human gut is possible.…”

Section: Plasmidsmentioning

confidence: 98%

“…The spread to humans can happen through direct contact with water or aquatic organisms, through drinking water, through handling or consumption of aquaculture products (Suzuki et al 2008). Direct spread from aquatic environments to humans can be involved human pathogens, such as V. cholera, V. vulnificus, Shigella species and Salmonella species or opportunistic pathogens, such as A. hydrophila, P. shigelloides, E. tarda and E. coli (Novais et al 2012;Vincent et al 2015). Thus, the occurrence of antimicrobial-resistant Salmonella species in aquatic environments is most likely attributable to contamination from human, animal or agriculture environments.…”

Section: Direct Spread Of Antibiotic Resistancementioning

confidence: 99%

Heavy use of antibiotics in aquaculture: Emerging human and animal health problems – A review

Manage

2018

Sri Lanka J. Aquat.

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Antibiotics have been extensively and effectively used in aquaculture due to accelerated growth of aquaculture and this has resulted in the development of serious health problems in aquaculture, other animals, and human. The use of a wide variety of antibiotics in large amounts, including non-biodegradable antibiotics, ensures that they remain in the aquatic environment for a long time. This has resulted; the emergence of antibiotic-resistant bacteria in the environments, an increase of antibiotic resistance in fish pathogens, transfer of these resistance determinants to bacteria and then land animals and finally become human pathogens along with alterations of the bacterial flora both in sediments and in the water column. Thus, the global efforts are needed to promote more judicious use of prophylactic antibiotics in aquaculture as accumulating evidence indicates that unrestricted use is detrimental to fish, terrestrial animals and human health.

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Different Genetic Supports for the tet (S) Gene in Enterococci

Cited by 17 publications

References 14 publications

Occurrence and distribution of tetracycline resistance determinants and their pollution profile in the aquaculture environment in Sri Lanka

Occurrence and distribution of tetracycline resistance determinants and their pollution profile in the aquaculture environment in Sri Lanka

Diversity of plasmids and Tn1546-type transposons among VanA Enterococcus faecium in Poland

Heavy use of antibiotics in aquaculture: Emerging human and animal health problems – A review

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