Different genes can be responsible for crown rot resistance at different developmental stages of wheat and barley

Yang, X.M.; Ma, Jun; Li, Haobing; Ma, Hongxiang; Jinbao, Yao; Liu, Chunji

doi:10.1007/s10658-010-9680-3

Cited by 48 publications

(44 citation statements)

References 20 publications

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“…To assess homoeolog expression bias in bread wheat during response to biotic stress, an established laboratory infection assay (Yang et al ., ) was performed to infect wheat seedlings with F. pseudograminearum . Four biological replicates of F. pseudograminearum ( Fp )‐inoculated and noninoculated (mock) wheat plants were sampled as described in Materials and Methods.…”

Section: Resultsmentioning

confidence: 99%

The defence‐associated transcriptome of hexaploid wheat displays homoeolog expression and induction bias

Powell

Fitzgerald

Stiller

et al. 2016

Plant Biotechnology Journal

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SummaryBread wheat (Triticum aestivum L.) is an allopolyploid species containing three ancestral genomes. Therefore, three homoeologous copies exist for the majority of genes in the wheat genome. Whether different homoeologs are differentially expressed (homoeolog expression bias) in response to biotic and abiotic stresses is poorly understood. In this study, we applied a RNA‐seq approach to analyse homoeolog‐specific global gene expression patterns in wheat during infection by the fungal pathogen Fusarium pseudograminearum, which causes crown rot disease in cereals. To ensure specific detection of homoeologs, we first optimized read alignment methods and validated the results experimentally on genes with known patterns of subgenome‐specific expression. Our global analysis identified widespread patterns of differential expression among homoeologs, indicating homoeolog expression bias underpins a large proportion of the wheat transcriptome. In particular, genes differentially expressed in response to Fusarium infection were found to be disproportionately contributed from B and D subgenomes. In addition, we found differences in the degree of responsiveness to pathogen infection among homoeologous genes with B and D homoeologs exhibiting stronger responses to pathogen infection than A genome copies. We call this latter phenomenon as ‘homoeolog induction bias’. Understanding how homoeolog expression and induction biases operate may assist the improvement of biotic stress tolerance in wheat and other polyploid crop species.

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Section: Resultsmentioning

confidence: 99%

The defence‐associated transcriptome of hexaploid wheat displays homoeolog expression and induction bias

Powell

Fitzgerald

Stiller

et al. 2016

Plant Biotechnology Journal

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“…In barley, loci affecting plant height are also located within the 3HL and 4H QTLs (Li et al ., ). The findings from the studies employing NILs showed that short isolines have stronger FCR resistance than do tall isolines (Ma et al ., ; Yang et al ., ; Zheng et al ., ). In addition, FCR infection seems to spread faster within tall plants, and this phenomenon was explained by the differences observed in cell densities between short and tall genotypes (Bai and Liu, ).…”

Section: Breeding For Fcr Resistance and Tolerancementioning

confidence: 99%

Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: recent progress and future prospects

Kazan

Gardiner

2018

Molecular Plant Pathology

203

158

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Diseases caused by Fusarium pathogens inflict major yield and quality losses on many economically important plant species worldwide, including cereals. Fusarium crown rot (FCR), caused by Fusarium pseudograminearum, is a cereal disease that occurs in many arid and semi-arid cropping regions of the world. In recent years, this disease has become more prevalent, in part as a result of the adoption of moisture-preserving cultural practices, such as minimum tillage and stubble retention. In this pathogen profile, we present a brief overview of recent research efforts that have not only advanced our understanding of the interactions between F. pseudograminearum and cereal hosts, but have also provided new disease management options. For instance, significant progress has been made in the genetic characterization of pathogen populations, the development of new tools for disease prediction, and the identification and pyramiding of loci that confer quantitative resistance to FCR in wheat and barley. In addition, transcriptome analyses have revealed new insights into the processes involved in host defence. Significant progress has also been made in understanding the mechanistic details of the F. pseudograminearum infection process. The sequencing and comparative analyses of the F. pseudograminearum genome have revealed novel virulence factors, possibly acquired through horizontal gene transfer. In addition, a conserved pathogen gene cluster involved in the degradation of wheat defence compounds has been identified, and a role for the trichothecene toxin deoxynivalenol (DON) in pathogen virulence has been reported. Overall, a better understanding of cereal host-F. pseudograminearum interactions will lead to the development of new control options for this increasingly important disease problem. Taxonomy: Fusarium pseudograminearum O'Donnell & Aoki; Kingdom Fungi; Phylum Ascomycota; Subphylum Pezizomycotina; Class Sordariomycetes; Subclass Hypocreomycetidae; Order Hypocreales; Family Nectriaceae; Genus Fusarium. Disease symptoms: Fusarium crown rot caused by F. pseudograminearum is also known as crown rot, foot rot and root rot. Infected seedlings can die before or after emergence. If infected seedlings survive, typical disease symptoms are browning of the coleoptile, subcrown internode, lower leaf sheaths and adjacent stems and nodal tissues; this browning can become evident within a few weeks after planting or throughout plant development. Infected plants may develop white heads with no or shrivelled grains. Disease symptoms are exacerbated under water limitation. Identification and detection: Fusarium pseudograminearum macroconidia usually contain three to five septa (22-60.5 × 2.5-5.5 μm). On potato dextrose agar (PDA), aerial mycelia appear floccose and reddish white, with red or reddish-brown reverse pigmentation. Diagnostic polymerase chain reaction (PCR) tests based on the amplification of the gene encoding translation elongation factor-1a (TEF-1a) have been developed for molecular identification. Host range: All maj...

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“…The variation among pathogenic isolates associated with the FCR complex has been previously studied by employing various assessment standards of inoculation techniques, including millet seeds, spore suspension and agar plugs (Akinsanmi et al, 2004;Fernandez and Chen, 2005;Smiley et al, 2005a;Mitter et al, 2006;Nicol et al, 2007;Li et al, 2008;Dyer et al, 2009;Gargouri-Kammoun et al, 2009;Yang et al, 2010;Poole et al, 2012). Most of these studies found that F. pseudograminearum and F. culmorum were the most pathogenic species, and F. avenaceum, F. equiseti and F. poae were the least pathogenic species.…”

Section: Introductionmentioning

confidence: 99%

Diversity of theFusariumpathogens associated with crown rot in the Huanghuai wheat‐growing region of China

Zhou

Wang

et al. 2019

Environmental Microbiology

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Summary To investigate the distribution and diversity of the pathogens associated with Fusarium crown rot in the Huanghuai wheat‐growing region (HHWGR) of China, we collected wheat samples with symptomatic stem bases from seven provinces in the HHWGR between 2013 and 2016. A total of 1196 isolates obtained from 222 locations were identified as 9 Fusarium species based on morphological and molecular identification. Of these pathogen species, F. pseudograminearum was the dominant species. Furthermore, F. sinensis was isolated from the disease specimens and tested for virulence to wheat. The result of the pathogenicity revealed that an intraspecific differentiation existed in F. pseudograminearum; sequence analysis of the EF‐1α gene showed that 194 F. pseudograminearum isolates were differentiated into two distinct clades which closed to the strains from Australia and China respectively, but neither pathogenicity nor EF‐1α sequence was related to the geographic origins of these isolates. However, universal rice primers‐polymerase chain reaction showed a correlation with the geographical origins of the 194 isolates, which were divided into eight subclusters, the level of genetic diversity was higher within a geographical population than among the different populations. The results of these analyses can be directly used to facilitate disease monitoring and development of control strategies.

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Different genes can be responsible for crown rot resistance at different developmental stages of wheat and barley

Cited by 48 publications

References 20 publications

The defence‐associated transcriptome of hexaploid wheat displays homoeolog expression and induction bias

The defence‐associated transcriptome of hexaploid wheat displays homoeolog expression and induction bias

Fusarium crown rot caused by Fusarium pseudograminearum in cereal crops: recent progress and future prospects

Diversity of theFusariumpathogens associated with crown rot in the Huanghuai wheat‐growing region of China

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