Different Functionalization of the Internal and External Surfaces in Mesoporous Materials for Biosensing Applications Using “Click” Chemistry

Guan, Bin; Ciampi, Simone; Saux, Guillaume Le; Gaus, Katharina; Reece, Peter J.; Gooding, J. Justin

doi:10.1021/la102599m

Cited by 54 publications

(61 citation statements)

References 52 publications

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“…Currently, thermal oxidation is the most popular stabilization process for PSi. Whereas such stabilization is crucial for optoelectronic technologies, its necessity for biological applications is yet uncertain [15][16][17][18][19]. In particular, it is unclear whether the direct derivatization of a fresh PSi surface is adequate for subsequent biological experiments.…”

Section: Introductionmentioning

confidence: 99%

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Naveas

Costa

Gallach

et al. 2012

Science and Technology of Advanced Materials

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Porous silicon (PSi) is widely used in biological experiments, owing to its biocompatibility and well-established fabrication methods that allow tailoring its surface. Nevertheless, there are some unresolved issues such as deciding whether the stabilization of PSi is necessary for its biological applications and evaluating the effects of PSi stabilization on the surface biofunctionalization with proteins. In this work we demonstrate that non-stabilized PSi is prone to detachment owing to the stress induced upon biomolecular adsorption. Biofunctionalized non-stabilized PSi loses the interference properties characteristic of a thin film, and groove-like structures resulting from a final layer collapse were observed by scanning electron microscopy. Likewise, direct PSi derivatization with 3-aminopropyl-triethoxysilane (APTS) does not stabilize PSi against immunoglobulin biofunctionalization. To overcome this problem, we developed a simple chemical process of stabilizing PSi (CoxPSi) for biological applications, which has several advantages over thermal stabilization (ToxPSi). The process consists of chemical oxidation in H 2 O 2 , surface derivatization with APTS and a curing step at 120• C. This process offers integral homogeneous PSi morphology, hydrophilic surface termination (contact angle θ = 26• ) and highly efficient derivatized and biofunctionalized PSi surfaces (six times more efficient than ToxPSi). All these features are highly desirable for biological applications, such as biosensing, where our results can be used for the design and optimization of the biomolecular immobilization cascade on PSi surfaces.

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Section: Introductionmentioning

confidence: 99%

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Naveas

Costa

Gallach

et al. 2012

Science and Technology of Advanced Materials

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“…Selective derivatization of the acetylene film by click chemistry in the absence or presence of ligand (TMEDA), and the subsequent biofunctionalization by cell adhesive peptide GRGDS (Ref. [98]; DSC = N,N'-disuccinimidyl carbonate, DMAP = 4-dimethylaminopyridine). of a wide range of organic functional groups, thus facilitating the orthogonal modification of biological entities onto silicon platforms.…”

Section: Resultsmentioning

confidence: 99%

Click Chemistry‐Based Functionalization on Non‐Oxidized Silicon Substrates

Cai

2011

Chemistry An Asian Journal

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Copper-catalyzed azide-alkyne cycloaddition (CuAAC), combined with the chemical stability of the SiÀC-bound organic layer, serves as an efficient tool for the modification of silicon substrates, particularly for the immobilization of complex biomolecules. This review covers recent advances in the preparation of alkynyl-or azido-terminated "clickable" platforms on non-oxidized silicon and their further derivatization by means of the CuAAC reaction. The exploitation of these "click"-functionalized organic thin films as model surfaces to study many biological events was also addressed, as they are directly relevant to the on-going effort of creating siliconbased molecular electronics and chemical/biomolecular sensors.

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“…The azide compounds, 1-azido-octane and 11-azido-3,6,9-trioxaundecan-1-ol were synthesized as described previously. 38 …”

Section: Generalmentioning

confidence: 99%

Mesoporous silicon photonic crystal microparticles: towards single-cell optical biosensors

et al. 2011

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In this paper we demonstrate the possibility of modifying porous silicon (PSi) particles with surface chemistry and recognition molecules (antibodies) such that these devices could potentially be used for singlecell identification or sensing. This is achieved by modifying PSi Rugate filters via hydrosilylation with surface chemistry that serves firstly, to protect the silicon surfaces from oxidation; secondly, renders the surfaces resistant to nonspecific adsorption of proteins and cells and thirdly, allows further functionality to be added such as the coupling of antibodies. The surface chemistry remained unchanged after sonication of the PSi to form PSi microparticles. The ability to monitor the spectroscopic properties of microparticles, and shifts in the optical signature due to changes in the refractive index of the material within the pore space, is demonstrated. The particles are shown to remain stable in physiological buffers and human blood for longer than one week. Finally, the modification of the PSi particles with functional antibodies is achieved.

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Different Functionalization of the Internal and External Surfaces in Mesoporous Materials for Biosensing Applications Using “Click” Chemistry

Cited by 54 publications

References 52 publications

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin

Click Chemistry‐Based Functionalization on Non‐Oxidized Silicon Substrates

Mesoporous silicon photonic crystal microparticles: towards single-cell optical biosensors

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