Different forms of apolipophorin III in Galleria mellonella larvae challenged with bacteria and fungi

Zdybicka-Barabas, Agnieszka; Sowa-Jasiłek, Aneta; Stączek, Sylwia; Jakubowicz, Teresa; Cytryńska, Małgorzata

doi:10.1016/j.peptides.2014.12.013

Cited by 19 publications

(8 citation statements)

References 57 publications

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“…This was surprising because apoLp-III is considered as a PRR molecule capable of interacting with various PAMPs, e.g., LTAs, LPS and β-1,3-glucan, which are components of the cell walls of Gram-positive and Gram-negative bacteria and fungi. The ability of apoLp-III to bind to the conidia of different filamentous fungi, including A. niger , yeast cells, as well as cells of different Gram-positive and Gram-negative bacteria has been demonstrated [ 65 , 66 , 67 , 68 , 69 , 70 , 71 ].…”

Section: Discussionmentioning

confidence: 99%

Fungal α-1,3-Glucan as a New Pathogen-Associated Molecular Pattern in the Insect Model Host Galleria mellonella

et al. 2021

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Recognition of pathogen-associated molecular patterns (PAMPs) by appropriate pattern recognition receptors (PRRs) is a key step in activating the host immune response. The role of a fungal PAMP is attributed to β-1,3-glucan. The role of α-1,3-glucan, another fungal cell wall polysaccharide, in modulating the host immune response is not clear. This work investigates the potential of α-1,3-glucan as a fungal PAMP by analyzing the humoral immune response of the greater wax moth Galleria mellonella to Aspergillus niger α-1,3-glucan. We demonstrated that 57-kDa and 61-kDa hemolymph proteins, identified as β-1,3-glucan recognition proteins, bound to A. niger α-1,3-glucan. Other hemolymph proteins, i.e., apolipophorin I, apolipophorin II, prophenoloxidase, phenoloxidase activating factor, arylphorin, and serine protease, were also identified among α-1,3-glucan-interacting proteins. In response to α-1,3-glucan, a 4.5-fold and 3-fold increase in the gene expression of antifungal peptides galiomicin and gallerimycin was demonstrated, respectively. The significant increase in the level of five defense peptides, including galiomicin, corresponded well with the highest antifungal activity in hemolymph. Our results indicate that A. niger α-1,3-glucan is recognized by the insect immune system, and immune response is triggered by this cell wall component. Thus, the role of a fungal PAMP for α-1,3-glucan can be postulated.

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Section: Discussionmentioning

confidence: 99%

Fungal α-1,3-Glucan as a New Pathogen-Associated Molecular Pattern in the Insect Model Host Galleria mellonella

et al. 2021

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“…Apolipophorin III, occurring in a diversity of insect species, increases the capacity of lipophorins to bind the lipid for delivery, and thereby facilitates energy generation. In Galleria mellonella , Chinese oak silkworm and Tribolium castaneum , apoLp‐III played crucial role in lipid transport and immune response against bacteria, fungi, mosquito, and Bacillus thuringiensis toxic protein (Gupta et al., ; Liu et al., ; Zdybicka‐Barabas, Sowa‐Jasiłek, Stączek, Jakubowicz, & Cytryńska, ). Thus, reduced expression of apolipophorins was likely to indicate lower lipolytic activity and reduced resistance in COTI‐fed insects.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis provides insights of anti‐insect molecular mechanism of Cassia obtusifolia trypsin inhibitor against Pieris rapae

Xiang

Zhang

Yin

et al. 2017

Arch Insect Biochem Physiol

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Pieris rapae, a serious Lepidoptera pest of cultivated crucifers, utilizes midgut enzymes to digest food and detoxify secondary metabolites from host plants. A recombinant trypsin inhibitor (COTI)1 from nonhost plant, Cassia obtusifolia, significantly decreased activities of trypsin-like proteases in the larval midgut on Pieris rapae and could suppress the growth of larvae. In order to know how COTI took effect, transcriptional profiles of P. rapae midgut in response to COTI was studied. A total of 51,544 unigenes were generated and 45.86% of which had homologs in public databases. Most of the regulated genes associated with digestion, detoxification, homeostasis, and resistance were downregulated after ingestion of COTI. Meanwhile, several unigenes in the integrin signaling pathway might be involved in response to COTI. Furthermore, using comparative transcriptome analysis, we detected differently expressing genes and identified a new reference gene, UPF3, by qRT-polymerase chain reaction (PCR). Therefore, it was suggested that not only proteolysis inhibition, but also suppression of expression of genes involved in metabolism, development, signaling, and defense might account for the anti-insect resistance of COTI. K E Y W O R D SCassia obtusifolia, nonhost plant, physiological response, Pieris rapae, trypsin inhibitor

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“…Besiyeri (100 g) olarak 22 g buğday unu, 22 g buğday kepeği, 11 g süt tozu, 5.5 g kuru maya, 17 g mum, 11 mL gliserol ve 11 mL bal karışımı kullanıldı. Larvalardan 0.33 g ağırlığında, rengi kremsi ve 2-3 cm uzunluğunda olanlar çalışmaya dahil edildi [6][7][8] . G.mellonella yaşam döngüsü Şekil 1'de gösterildi.…”

Section: Galleria Mellonella Larvalarıunclassified

“…Mikroorganizma süspansiyonlarından 10 μL'si larvaların sol arka ayağı hizasından Hamilton iğnesi ile enjekte edildi 6,7 . Her grupta 12 larva olmak üzere; (1) ellenmemiş sağ-lıklı kontrol, (2) serum fi zyolojik enjekte edilmiş SF kontrol ve (3) patojen etken enjekte edilmiş deney grubu olarak ayrıldı.…”

Section: Larvalara Enjeksiyon Yapılmasıunclassified

Using Galleria mellonella as an In Vivo Model to Study the Virulence of Some Bacterial and Fungal Agents

Kalkancı¹,

Fouad²,

Erdoğan³

et al. 2015

Mikrobiyol Bul

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* Bu çalışma, XXXVI. Türk Mikrobiyoloji Kongresi (12-16 Kasım 2014, Antalya) ÖZMum güvesi olarak bilinen Galleria mellonella gibi omurgasız canlılar, mikroorganizmaların virülansı-nın ve konak yanıtının araştırılmasında kullanılmaktadır. Bu canlılar ekonomik olmaları, etik kurul onayı gerektirmemeleri ve kolayca uygulama yapılabilmeleri nedeniyle avantajlıdır. Bu makalede, Galleria mellonella larvalarının bazı bakteri ve mantarlar ile enfekte edilmesiyle oluşturulan deneysel bir in vivo çalışma sunulmaktadır. Çalışmada, genişlemiş spektrumlu beta-laktamaz (GSBL) üreten ve üretmeyen Escherichia coli, Klebsiella pneumoniae ve Pseudomonas aeruginosa klinik kökenleri ile kolistine dirençli ve duyarlı Acinetobacter baumanii klinik kökenleri; Candida albicans (ATCC 10231), Scedosporium aurantiacum (CBS 136047), Pseudallescheria boydii (CBS 117410) standart kökenleri, Aspergillus terreus ve Fusarium oxysporum klinik kökenleri kullanılmıştır. Bu bakteriler ve mantarlar ile enfekte edilen larvalarda mortalite oranları Kaplan-Meier grafi kleri kullanılarak hesaplanmıştır. Deneyin 16. saatinde mortalite oranı; GSBL üreten ve üretmeyen E.coli, GSBL üretmeyen K.pneumoniae ve GSBL üreten P.aeruginosa ile enfekte larvalarda %83; GSBL üreten K.pneumoniae ile enfekte larvalarda %91; GSBL üretmeyen P.aeruginosa ile enfekte larvalarda %75; kolistine dirençli ve duyarlı A.baumanii ile enfekte larvalarda %66 olarak bulunmuştur. Bakteriler ile enfekte edilen larvaların 24 saatteki mortalite oranları %100 olup, mantarlar ile enfekte edilen larvalardan daha yüksek bulunmuştur. Deneyin 16. saatinde mantarlar ile enfekte larvalar için mortalite oranları; C.albicans ve F.oxysporum için %0, S.aurantiacum için %16, P.boydii ve A.terreus için %8; 24. saatinde C.albicans ve P.boydii için %25, S.aurantiacum, A.terreus ve F.oxysporum için %33; 48.

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Different forms of apolipophorin III in Galleria mellonella larvae challenged with bacteria and fungi

Cited by 19 publications

References 57 publications

Fungal α-1,3-Glucan as a New Pathogen-Associated Molecular Pattern in the Insect Model Host Galleria mellonella

Fungal α-1,3-Glucan as a New Pathogen-Associated Molecular Pattern in the Insect Model Host Galleria mellonella

Comparative transcriptome analysis provides insights of anti‐insect molecular mechanism of Cassia obtusifolia trypsin inhibitor against Pieris rapae

Using Galleria mellonella as an In Vivo Model to Study the Virulence of Some Bacterial and Fungal Agents

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