Different expression patterns of pAkt, NF‐κB and cyclin D1 proteins during the invasion process of head and neck squamous cell carcinoma: an <i>in vitro</i> approach

Giudice, Fernanda Salgueiredo; Vechio, Aluana Maria da Costa Dal; Abrahão, Aline Corrêa; Sperandio, Felipe Fornias; Pinto, Décio dos Santos

doi:10.1111/j.1600-0714.2010.00960.x

Cited by 17 publications

(17 citation statements)

References 38 publications

(33 reference statements)

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“…Images were quantified as the following: four photographs (same magnitude and resolution) of either the laser or control group were randomly selected and set in RGB mode to produce a color histogram that was based on the pixels` brightness in an arbitrary proportional scale between the experimental groups [27]. …”

Section: Methodsmentioning

confidence: 99%

Low‐level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

Sperandio

Simões

Corrêa

et al. 2014

Journal of Biophotonics

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Low-level laser therapy (LLLT) has been extensively employed to improve epithelial wound healing, though the exact response of epithelium maturation and stratification after LLLT is unknown. Thus, this study aimed to assess the in vitro growth and differentiation of keratinocytes (KCs) and in vivo wound healing response when treated with LLLT. Human KCs (HaCaT cells) showed an enhanced proliferation with all the employed laser energy densities (3, 6 and 12 J/cm2, 660nm, 100mW), together with an increased expression of Cyclin D1. Moreover, the immunoexpression of proteins related to epithelial proliferation and maturation (p63, CK10, CK14) all indicated a faster maturation of the migrating KCs in the LLLT-treated wounds. In that way, an improved epithelial healing was promoted by LLLT with the employed parameters; this improvement was confirmed by changes in the expression of several proteins related to epithelial proliferation and maturation.

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Section: Methodsmentioning

confidence: 99%

Low‐level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

Sperandio

Simões

Corrêa

et al. 2014

Journal of Biophotonics

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“…IR exists as two isoforms, IR-A and IR-B derived from alternative pre-mRNA splicing. These isoforms differ by the inclusion or exclusion of exon 11, which alters the ligand binding affinity and downstream signaling properties of the IR [71–74]. Inclusion of exon 11 yields IR-B, which is highly expressed in known insulin-target organs, such as liver, muscle, and adipose tissue and has a high affinity for insulin [71–73**].…”

Section: Igf1 Receptor (Igf1r) As the Primary Mediator Of Igf1 Actionmentioning

confidence: 99%

Insulin-like growth factor 1

Bortvedt

Lund

2012

Current Opinion in Gastroenterology

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Purpose of review To summarize recent evidence that IGF1 mediates growth effects of multiple trophic factors and discuss clinical relevance. Recent findings Recent reviews and original reports indicate benefits of growth hormone (GH) and long-acting glucagon-like peptide 2 (GLP2) analogues in short bowel syndrome and Crohn’s disease. This review highlights evidence that biomarkers of sustained small intestinal growth or mucosal healing and evaluation of intestinal epithelial stem cell biomarkers may improve clinical measures of intestinal growth or response to trophic hormones. Compelling evidence that IGF1 mediates growth effects of GH and GLP2 on intestine or linear growth in preclinical models of resection or Crohn’s disease is presented, along with a concept that these hormones or IGF1 may enhance sustained growth if given early after bowel resection. Evidence that SOCS protein induction by GH or GLP2 in normal or inflamed intestine, may limit IGF1-induced growth, but protect against risk of dysplasia or fibrosis is reviewed. Whether IGF1 receptor mediates IGF1 action and potential roles of insulin receptors are addressed. Summary IGF1 has a central role in mediating trophic hormone action in small intestine. Better understanding of benefits and risks of IGF1, receptors that mediate IGF1 action, and factors that limit undesirable growth are needed.

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“…The Akt/NF-κB signaling pathway plays a crucial role in regulating the invasive phenotype of oral cancer 23 . In light of the reported activity of FTY720 on inhibiting PI3K-mediated Akt phosphorylation in human hepatocellular carcinoma 13 , we assessed the effect of FTY720 on markers associated with this signaling axis.…”

Section: Resultsmentioning

confidence: 99%

FTY720 Induces Autophagy-Associated Apoptosis in Human Oral Squamous Carcinoma Cells, in Part, through a Reactive Oxygen Species/Mcl-1-Dependent Mechanism

Chiu

et al. 2017

Sci Rep

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In this study, we interrogated the mechanism by which the immunosuppressant FTY720 mediates anticancer effects in oral squamous cell carcinoma (OSCC) cells. FTY720 differentially suppressed the viability of the OSCC cell lines SCC4, SCC25, and SCC2095 with IC50 values of 6.1, 6.3, and 4.5 μM, respectively. This antiproliferative effect was attributable to the ability of FTY720 to induce caspase-dependent apoptosis. Mechanistic evidence suggests that FTY720-induced apoptosis was associated with its ability to inhibit Akt-NF-κB signaling, to facilitate the proteasomal degradation of the antiapoptotic protein Mcl-1, and to increase reactive oxygen species (ROS) generation. Both overexpression of Mcl-1 and inhibition of ROS partially protected cells from FTY720-induced caspase-9 activation, PARP cleavage and cytotoxicity. In addition, FTY720 induced autophagy in OSCC cells, as manifested by LC3B-II conversion, decreased p62 expression, and accumulation of autophagosomes. Inhibition of autophagy by bafilomycin A1 protected cells from FTY720-induced apoptosis. Together, these findings suggest an intricate interplay between autophagy and apoptosis in mediating the tumor-suppressive effect in OSCC cells, which underlies the translational potential of FTY720 in fostering new therapeutic strategies for OSCC.

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Different expression patterns of pAkt, NF‐κB and cyclin D1 proteins during the invasion process of head and neck squamous cell carcinoma: an in vitro approach

Abstract: This study suggests the participation of pAkt, NF-κB and cyclin D1 in the invasion process of head and neck squamous cell carcinoma. Moreover, cytoplasmic pAkt network-like structure was probably related to cytoskeleton changes presented during invasion.

Cited by 17 publications

References 38 publications

Low‐level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

Low‐level laser irradiation promotes the proliferation and maturation of keratinocytes during epithelial wound repair

Insulin-like growth factor 1

FTY720 Induces Autophagy-Associated Apoptosis in Human Oral Squamous Carcinoma Cells, in Part, through a Reactive Oxygen Species/Mcl-1-Dependent Mechanism

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