Different expression patterns of<i>LGALS1</i>and<i>LGALS3</i>in polycythemia vera, essential thrombocythemia and primary myelofibrosis

Moura, Lívia Gonzaga; Tognon, Raquel; Nunes, Natália S.; Rodrigues, Lílian Cataldi; Ferreira, Ademar; Kashima, Simone; Covas, Dimas Tadeu; Santana, Mary Elizabeth de; Souto, Elizabeth Xisto; Perobelli, Leila Martins; Simões, Belinda Pinto; Dias‐Baruffi, Marcelo; Castro, Fabíola Attié de

doi:10.1136/jclinpath-2016-203948

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…Some of these molecules (for example, IL-8, CCL2, PLAUR, SPP1 and LGALS3) showed higher plasma levels in PMF patients compared to HDs. Although CCL2, 26 IL-8 24 and PLAUR 27 have been already reported to be increased in PMF patients in comparison with HDs, no data were available for SPP1 and LGALS3, 28 , 29 respectively. However, both these molecules have been associated with other inflammatory disorders.…”

Section: Discussionmentioning

confidence: 99%

Involvement of MAF/SPP1 axis in the development of bone marrow fibrosis in PMF patients

et al. 2017

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Primary myelofibrosis (PMF) is a myeloproliferative neoplasm characterized by hyperplastic megakaryopoiesis and myelofibrosis. We recently described the upregulation of MAF (v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog) in PMF CD34+ hematopoietic progenitor cells (HPCs) compared to healthy donor. Here we demonstrated that MAF is also upregulated in PMF compared with the essential thrombocytemia (ET) and polycytemia vera (PV) HPCs. MAF overexpression and knockdown experiments shed some light into the role of MAF in PMF pathogenesis, by demonstrating that MAF favors the megakaryocyte and monocyte/macrophage commitment of HPCs and leads to the increased expression of proinflammatory and profibrotic mediators. Among them, we focused our further studies on SPP1 and LGALS3. We assessed SPP1 and LGALS3 protein levels in 115 PMF, 47 ET and 24 PV patients plasma samples and we found that SPP1 plasma levels are significantly higher in PMF compared with ET and PV patients. Furthermore, in vitro assays demonstrated that SPP1 promotes fibroblasts and mesenchymal stromal cells proliferation and collagen production. Strikingly, clinical correlation analyses uncovered that higher SPP1 plasma levels in PMF patients correlate with a more severe fibrosis degree and a shorter overall survival. Collectively our data unveil that MAF overexpression contributes to PMF pathogenesis by driving the deranged production of the profibrotic mediator SPP1.

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Section: Discussionmentioning

confidence: 99%

Involvement of MAF/SPP1 axis in the development of bone marrow fibrosis in PMF patients

et al. 2017

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“…In multiple myeloma, galectin-1 displayed dual functions depending on CD45RA expression of malignant cells: it promoted viability and proliferation of CD45RA − cells through aggregation of β1-integrin, and induced growth arrest of CD45RA + cells through inhibition of ERK phosphorylation ( 117 ). Aberrant expression of galectin-1 and/or−3 in patients with MPNs is also discovered to correlate with JAK2 mutation in these diseases and state of cell differentiation ( 118 ).…”

Section: Lectinsmentioning

confidence: 99%

Multiple Roles of Glycans in Hematological Malignancies

Pang

Guan

et al. 2018

Front. Oncol.

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The three types of blood cells (red blood cells for carrying oxygen, white blood cells for immune protection, and platelets for wound clotting) arise from hematopoietic stem/progenitor cells in the adult bone marrow, and function in physiological regulation and communication with local microenvironments to maintain systemic homeostasis. Hematological malignancies are relatively uncommon malignant disorders derived from the two major blood cell lineages: myeloid (leukemia) and lymphoid (lymphoma). Malignant clones lose their regulatory mechanisms, resulting in production of a large number of dysfunctional cells and destruction of normal hematopoiesis. Glycans are one of the four major types of essential biological macromolecules, along with nucleic acids, proteins, and lipids. Major glycan subgroups are N-glycans, O-glycans, glycosaminoglycans, and glycosphingolipids. Aberrant expression of glycan structures, resulting from dysregulation of glycan-related genes, is associated with cancer development and progression in terms of cell signaling and communication, tumor cell dissociation and invasion, cell-matrix interactions, tumor angiogenesis, immune modulation, and metastasis formation. Aberrant glycan expression occurs in most hematological malignancies, notably acute myeloid leukemia, myeloproliferative neoplasms, and multiple myeloma, etc. Here, we review recent research advances regarding aberrant glycans, their related genes, and their roles in hematological malignancies. Our improved understanding of the mechanisms that underlie aberrant patterns of glycosylation will lead to development of novel, more effective therapeutic approaches targeted to hematological malignancies.

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“…Cancer, one of the major causes of death in the world, is a complex genetic disease characterized by uncontrolled cell growth, metastasis and angiogenesis [2]. Chemotherapy and radiotherapy among the applied cancer treatments lead to high costs and many side effects.…”

mentioning

confidence: 99%

In vitro antiproliferative, antioxidant and anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz

Şen¹,

Göğer²,

Özakpınar³

et al. 2022

jrp

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In this study, methanol extract (CSM) and their hexane (CSH), chloroform (CSC), ethyl acetate (CSEA) and aqueous methanol fractions (CSAM) prepared from aerial parts of Centaurea saligna (K.Koch) Wagenitz were investigated for in vitro antiproliferative, anti-inflammatory and antioxidant activity. Anticancer, antioxidant, antiinflammatory activities of these extracts were carried out by MTT, DPPH-ABTS, 5-lipoxygenase methods, respectively. Methanol extract and its fractions of C. saligna were evaluated for their cytotoxicity against MCF-7, A549, HeLa, HT-29, PC-3 cell lines at a concentration of 50 µg / ml. Total flavonoid and phenolic contents of extracts were detected by AlCI3 and Folin-Ciocalteu methods, respectively. Analysis of phytochemical of CSEA, showing a strong anti-inflammatory activity with good antioxidant activity, was performed by LC-MS/MS. CSC exhibited the best antiproliferative activity against HeLa, HT-29, MCF-7 cell lines with 50.18%, 46.88%, 45.42% mortality, respectively. CSEA showed the highest antioxidant activity with IC50 values of 82.05 µg/ml and 108.4 µg/ml against ABTS and DPPH radicals, respectively. The highest total phenolic amounts have been determined in CSEA with value of 379.2 mg GAE/g extract. In the same way, the highest total flavonoid amounts have been observed in CSEA with values of 170.3 mg QE/g extract. CSEA showed strong anti-inflammatory activity with IC50 value of 0.10 µg/ml when compared to indomethacine as standard (22.39 µg/ml). Analysis of CSEA by LC-MS/MS revealed that the major compounds were quinic acid, 5-caffeoylquinic acid, apigenin C-hexoside-C-pentoside, p-coumaroylquinic acid, quercetin glucoside, di-caffeoylquinic acid, isorhamnetine glucoside, isorhamnetin glucuronide and isorhamnetin derivative. These results proved that CSEA has significant anti-inflammatory and antioxidant activity and CSC has good antiproliferatif activity. Also, the results demostrate that CSEA and CSC are a good source for further bioactivity-guided isolation in discovering new active antioxidant, anti-inflammatory and antiproliferative compounds.

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Different expression patterns ofLGALS1andLGALS3in polycythemia vera, essential thrombocythemia and primary myelofibrosis

Cited by 3 publications

References 17 publications

Involvement of MAF/SPP1 axis in the development of bone marrow fibrosis in PMF patients

Involvement of MAF/SPP1 axis in the development of bone marrow fibrosis in PMF patients

Multiple Roles of Glycans in Hematological Malignancies

In vitro antiproliferative, antioxidant and anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz

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