Different expression of angiogenesis-related factors between human cultivated corneal and oral epithelial sheets

Expression of Angiogenesis-Related Factors in Human Corneas after Cultivated Oral Mucosal Epithelial Transplantation

“…19 The same group indicated that TSP-1 also is the only antiangiogenic factor expressed solely by the corneal, but not conjunctival epithelia. 20 In our study, TSP-1 was not detected in normal conjunctiva and oral mucosa, but was detected beneath normal corneal epithelium, consistent with the findings of Sekiyama et al 20 and others. 24 Negative staining of keratin 8 and TSP-1 in almost all specimens suggested an oral mucosa-originated epithelia, and explains the inferior antiangiogenic property of COMET.…”

“…Numerous studies have focused on the mechanisms of corneal NV following LSCD 17,18 or other etiologies, 29 while others have reported differential expression of angiogenesis-related factors in cultivated corneal (CCE) and oral mucosal epithelial cells (COE). [20][21][22] Additionally, miscellaneous angiogenesis-related factors have been localized immunohistochemically in human corneas, [22][23][24][25]30,31 oral mucosa, 22,[32][33][34][35] and vascularized corneas 23,36,37 (see Supplementary Table S2 for a literature review, http://www. iovs.org/lookup/suppl/d oi:10.1167/iovs.11-9293/-/ DCSupplemental).…”

“…[13][14][15][16] Clinically, corneal NV following total LSCD is the most severe type, and the molecular mechanisms regulating NV formation and restoration of corneal avascularity after CLET have been reviewed extensively. 17,18 Recently, in vitro studies using cultivated oral and corneal epithelial cells from either humans [19][20] or rabbits 20,21 have shown that decreased expression of thrombospondin-1 (TSP-1) 20 and soluble fms-like tyrosine kinase-1 (sFlt-1), 22 and increased secretion of fibroblast growth factor-2 (FGF-2) 21 may be responsible for the enhanced angiogenic potential after COMET. Until now, clinicopathologic studies focusing on angiogenesis-related factors in patients receiving COMET have been lacking.…”

Transplantation of cultivated oral mucosal epithelial cells for severe corneal burn

“…In our experience, the blood vessels may even reach the central cornea (Figures 2g and 4f). For this, Sekiyama et al 29 suggested that decreased secretion of thrombospondin (TSP)-1 and increased secretion of fibroblast growth factor (FGF)-2 by cultivated oral mucosal epithelial cells may be responsible for the inferior antiangiogenic Figure 4 Representative photographs of patient 3. A 25-year-old man suffered from alkaline burn of right eye with 360 degrees of limbal ischaemia.…”

In vitro and in vivo differentiation of human embryonic stem cells into retina‐like organs and comparison with that from mouse pluripotent epiblast stem cells

“…Thus, hESCs are expected to substitute for the human fetus, at least in part, to unravel the mystery of human-specific developmental mechanisms (Cauffman et al, 2006(Cauffman et al, , 2009Choudhary et al, 2007;Dvash et al, 2004Dvash et al, , 2006Gjerstorff et al, 2008;Pera and Trounson, 2004;Rugg-Gunn et al, 2005). Even restricted to the ocular cells or tissues, only cultured cornea cell sheets induced from epithelial stem cells have been used for therapy (Nozaki et al, 2008;Sekiyama et al, 2006), and many induced cell types including those of retina and retinal pigmented epithelium have been used only in animal models (Kubota et al, 2006;MacLaren et al, 2006;Wang et al, 2008). Still, ocular cells good for therapeutic use may possibly be prepared from ES cells or iPS cells; however, the complex layered structure of the retina would be hard to regenerate as a tissue in present culture systems.…”